A5033204062- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Glycosylation and Glycoproteins Research
- Microfluidic and Capillary Electrophoresis Applications
- Metal-Organic Frameworks: Synthesis and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Sensor Technologies
- Bacteriophages and microbial interactions
- Analytical Chemistry and Chromatography
- Cancer-related gene regulation
Université de Strasbourg
2018-2024
Institut de Biologie Moléculaire et Cellulaire
2022-2024
Architecture et Réactivité de l'arN
2022-2024
Centre National de la Recherche Scientifique
2018-2024
Institut de génétique et de biologie moléculaire et cellulaire
2023-2024
Integrated Structural Biology Grenoble
2023-2024
Inserm
2023
Transgene (France)
2022
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2017
Institut de Chimie
2017
The MODOMICS database was updated with recent data and now includes new types related to RNA modifications. Changes the include an expanded modification catalog, encompassing both natural synthetic residues identified in structures. This addition aids representing sequences from RCSB PDB more effectively. To manage increased number of modifications, adjustments nomenclature system were made. Updates section reintroduction sequence alignments for tRNAs rRNAs. protein connected structures...
Capillary zone electrophoresis-mass spectrometry (CE-MS) is a mature analytical tool for the efficient profiling of (highly) polar and ionizable compounds. However, use CE-MS in comparison to other separation techniques remains underrepresented metabolomics, as this approach still perceived technically challenging less reproducible, notably migration time. The latter key reliable metabolic profiles unknown biomarker identification that complementary high resolution MS/MS. In work, we present...
Capillary electrophoresis-mass spectrometry coupling is a growing technique in biopharmaceutics characterization. Assessment of monoclonal antibodies well known at middle-up and bottom-up levels to obtain information about the sequence, post-translational modifications degradation products. Intact protein analysis an actual challenge be closer real structure. At this level, techniques are time consuming or cumbersome processes. In work, 20 minutes separation method has been developed...
Over the past decade there has been a growing interest in RNA modification analysis. High performance liquid chromatography–tandem mass spectrometry coupling (HPLC–MS/MS) is classically used to characterize post-transcriptional modifications of ribonucleic acids (RNAs). Here we propose novel and simple workflow based on capillary zone electrophoresis–tandem (CE–MS/MS), positive mode, at nucleoside oligonucleotide levels. By first totally digesting purified RNA, prior CE–MS/MS analysis, were...
As RNA post-transcriptional modifications are of growing interest, several methods were developed for their characterization. One them established identification, at the nucleosidic level, is hyphenation separation methods, such as liquid chromatography or capillary electrophoresis, to tandem mass spectrometry. However, our knowledge, no software yet available untargeted identification from MS/MS data-dependent acquisitions. Thus, very long and tedious manual data interpretations required....
Analysis of the profile tRNA modifications in several Archaea allowed us to observe a novel modified uridine V-loop tRNAs from two species: Pyrococcus furiosus and Sulfolobus acidocaldarius Recently, Ohira colleagues characterized 2'-phosphouridine (Up) at position 47 thermophilic Sulfurisphaera tokodaii, as well other archaea bacteria. From presence gene arkI corresponding RNA kinase responsible for Up47 formation, they also concluded that should be present Reanalysis our earlier data...
Transfer RNA (tRNA) modifications are essential for the temperature adaptation of thermophilic and psychrophilic organisms, as they control rigidity flexibility transcripts. To further understand how specific tRNA adjusted to maintain functionality in response fluctuations, we investigated whether represent an bacteria different growth temperatures (minimal, optimal, maximal), focusing on closely related (P. halocryophilus E. sibiricum), mesophilic (B. subtilis), (G. stearothermophilus)...
Ribosomal RNA contains many posttranscriptionally modified nucleosides, particularly in the functional parts of ribosome. The distribution these modifications varies from one organism to another. In Bacillus subtilis , model for Gram-positive bacteria, mass spectrometry experiments revealed presence 7-methylguanosine (m 7 G) at position 2574 23S rRNA, which lies A-site peptidyl transferase center large ribosomal subunit. Testing several m G methyltransferase candidates allowed us identify...
Transfer RNA (tRNA) modifications are essential for the temperature adaptation of thermophilic and psychrophilic organisms as they control rigidity flexibility transcripts. To further understand how specific tRNA adjusted to maintain functionality in response fluctuations, we investigated whether represent an bacteria different growth temperatures (minimal, optimal, maximal), focusing on closely related (P. halocryophilus E. sibiricum), mesophilic (B. subtilis), (G. stearothermophilus)...
Summary The ribosomal RNA of the human protein synthesis machinery comprises numerous chemical modifications that are introduced during ribosome biogenesis. We present 1.9 Å resolution cryo-EM structure 80S resolving new rRNA and functionally important ions such as Zn 2+ , K + Mg including their associated individual water molecules. 2’-O-methylation, pseudo-uridine base were confirmed by mass spectrometry resulting in a complete investigation > 230 sites many which could not be addressed...