A5022007302- Microtubule and mitosis dynamics
- Reproductive Biology and Fertility
- Genetics, Aging, and Longevity in Model Organisms
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Carcinogens and Genotoxicity Assessment
- Epigenetics and DNA Methylation
- Sperm and Testicular Function
- Protein Kinase Regulation and GTPase Signaling
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Cancer and Skin Lesions
- Ovarian function and disorders
- Signaling Pathways in Disease
- Heat shock proteins research
- Enzyme Structure and Function
- Osteoarthritis Treatment and Mechanisms
- Estrogen and related hormone effects
- Curcumin's Biomedical Applications
- Tumors and Oncological Cases
- Vascular Tumors and Angiosarcomas
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Protist diversity and phylogeny
- Histiocytic Disorders and Treatments
Université Paris Cité
2023
Institut Jacques Monod
2023
Institut de Biologie Paris-Seine
2020-2022
Laboratoire de Biologie du Développement
2006-2022
Centre National de la Recherche Scientifique
2002-2021
Sorbonne Université
2001-2020
Columbia University
2006-2008
Cancer Research Center
2007
In many cell types, the mitogen-activated protein kinase (MAPK) also named extracellular signal-regulated (ERK) is activated in response to a variety of growth factor-receptor interactions and leads transcriptional activation immediate early genes, hereby influencing number tissue-specific biological activities, as proliferation, survival differentiation. one specific type however, female germ cell, MAPK does not follow this canonical scheme. oocytes, independently factors tyrosine...
Ovulated eggs possess maternal apoptotic execution machinery that is inhibited for a limited time. The fertilized switch off this time bomb whereas aged unfertilized and parthenogenetically activated fail to stop the timer die. To investigate nature of molecular clock triggers egg decision committing suicide, we introduce here Xenopus as an in vivo system studying death eggs. We report after ovulation, number remains female body where they die by apoptosis. Similarly, ovulated recovered...
Entry into mitosis or meiosis relies on the coordinated action of kinases and phosphatases that ultimately leads to activation Cyclin B-Cdk1, also called MPF for M-phase promoting factor. Vertebrate oocytes are blocked in prophase first meiotic division, an arrest tightly controlled by a high PKA activity. Reentry depends Cdk1 obeys two steps mechanism: catalytic amount is generated protein synthesis-dependent manner; then regulatory network auto-amplification loop initiated. This second...
Vertebrate oocytes proceed through the 1st and 2nd meiotic division without intervening S-phase to become haploid. Although DNA replication does not take place, unfertilized acquire competence replicate one hour after division, by accumulating an essential factor of replicative machinery, Cdc6. Here, we discovered that turnover Cdc6 is precisely regulated in avoid inhibition Cdk1. At meiosis resumption, starts be expressed but cannot accumulate due a degradation mechanism activated During...
Abstract Oocytes are held in meiotic prophase for prolonged periods until hormonal signals trigger divisions. Key players of M-phase entry the opposing Cdk1 kinase and PP2A-B55δ phosphatase. In Xenopus , protein Arpp19, phosphorylated at serine 67 by Greatwall, plays an essential role inhibiting PP2A-B55δ, promoting activation. Furthermore, Arpp19 has earlier maintaining arrest through a second (S109) PKA. Prophase release, induced progesterone, relies on dephosphorylation S109, owing to...
ABSTRACT Oocytes are held in meiotic prophase for prolonged periods until hormonal signals trigger divisions. Key players of M-phase entry the opposing Cdk1 kinase and PP2A-B55δ phosphatase. In Xenopus , protein Arpp19, phosphorylated at serine 67 by Greatwall, plays an essential role inhibiting PP2A-B55δ, promoting activation. Furthermore Arpp19 has earlier maintaining arrest through a second (S109) PKA. Prophase release, induced progesterone, relies on dephosphorylation S109, owing to...