A5058985498- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Pluripotent Stem Cells Research
- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- RNA Research and Splicing
- Biochemical and Structural Characterization
- Neuroscience and Neuropharmacology Research
- Exercise and Physiological Responses
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- Neurogenesis and neuroplasticity mechanisms
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Glycosylation and Glycoproteins Research
- Advanced Memory and Neural Computing
- Neuroscience and Neural Engineering
- Supramolecular Self-Assembly in Materials
- Advanced Proteomics Techniques and Applications
- Molecular Communication and Nanonetworks
Children's Medical Research Institute
2016-2024
The University of Sydney
2016-2024
Advances in the study of neurological conditions have been possible because pluripotent stem cell technologies and organoids. Studies described generation neural ectoderm-derived retinal brain structures from cells. However, field is still troubled by technical challenges, including high culture costs variability. Here, we describe a simple economical protocol that reproducibly gives rise to retina cortical regions confluent cultures The spontaneously generated organoids are...
Abstract Neuronal communication relies on the release of neurotransmitters from various populations synaptic vesicles. Despite displaying vastly different probabilities and mobilities, reserve recycling pool vesicles co-exist within a single cluster suggesting that small biomolecular condensates could regulate their nanoscale distribution. Here, we performed large-scale activity-dependent phosphoproteome analysis hippocampal neurons in vitro identified Tau as highly phosphorylated disordered...
Stable function of networks requires that synapses adapt their strength to levels neuronal activity, and failure do so results in cognitive disorders. How such homeostatic regulation may be implemented mammalian remains poorly understood. Here we show the phosphorylation status several positions active-zone (AZ) protein RIM1 are relevant for synaptic glutamate release. Position RIMS1045 is necessary sufficient expression silencing-induced plasticity kept phosphorylated by serine arginine...
Depolarization of presynaptic terminals stimulates calcium influx, which evokes neurotransmitter release and activates phosphorylation-based signalling. Here, we present the first global temporal profile activity-dependent phospho-signalling, includes two KCl stimulation levels analysis poststimulus period. We profiled 1,917 regulated phosphopeptides bioinformatically identified six patterns co-regulated proteins. The proteins with large changes in phospho-status were again prominently 7,070...
The clathrin heavy chain N-terminal domain interacts with endocytic adapter proteins via binding motifs to assemble triskelia into cages. However, the precise mechanism of assembly is not yet known. Clathrin protein AP180 has more than any other and a major role in coat during synaptic vesicle biogenesis. We now demonstrate that some previously identified may be non-functional non-conventional sequence influence on function. related protein, lymphoid myeloid leukemia (CALM), fewer functions...
Defining the molecular networks orchestrating human brain formation is crucial for understanding neurodevelopment and neurological disorders. Challenges in acquiring early tissue have incentivized use of three-dimensional pluripotent stem cell (hPSC)-derived neural organoids to recapitulate neurodevelopment. To elucidate programs that drive this highly dynamic process, here, we generate a comprehensive trans-omic map phosphoproteome, proteome, transcriptome exit pluripotency differentiation...
Abstract Dysferlin is a Ca 2+ -activated lipid binding protein implicated in muscle membrane repair. Recessive variants DYSF result dysferlinopathy, progressive muscular dystrophy. We showed previously that calpain cleavage within motif encoded by alternatively spliced exon 40a releases 72 kDa C-terminal minidysferlin recruited to injured sarcolemma. Herein we use CRISPR/Cas9 gene editing knock out murine Dysf 40a, specifically assess its role repair and development of dysferlinopathy....
Stable function of networks in the brain requires that synapses adapt their strength to levels neuronal activity a durable manner and failures do so result cognitive disorders. How such persistent homeostatic regulation synaptic connections may be biologically implemented mammalian remains poorly understood. Here, we have employed optical recordings vesicle release from single synapses, super-resolution microscopy, phosphoproteomics reveal level transmitter is stored phosphorylation state...
Abstract Advances in the study of neurological conditions have been possible due to induced pluripotent stem cell technologies and generation neural types organoids. Numerous studies described ectoderm-derived retinal brain structures from cells. However, field is still troubled by technical challenges, including high culture costs organoid-to-organoid variability. Here, we describe a simple economical protocol that reproducibly gives rise retina cortical regions confluent cultures The...