A5077876328- Botanical Research and Chemistry
- Plant-Microbe Interactions and Immunity
- Plant and fungal interactions
- Legume Nitrogen Fixing Symbiosis
- Genetic and Environmental Crop Studies
- Plant Molecular Biology Research
- Agricultural pest management studies
- Plant Disease Resistance and Genetics
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Biochemical and Structural Characterization
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Genomics, phytochemicals, and oxidative stress
- Plant Gene Expression Analysis
- Plant pathogens and resistance mechanisms
- NF-κB Signaling Pathways
- TGF-β signaling in diseases
- Trace Elements in Health
- Glycosylation and Glycoproteins Research
- Plant Reproductive Biology
- Monoclonal and Polyclonal Antibodies Research
- Photosynthetic Processes and Mechanisms
- Heavy metals in environment
- Genomics and Chromatin Dynamics
Commonwealth Scientific and Industrial Research Organisation
2014-2023
Agriculture and Food
2016-2023
Plant Industry
2002-2011
ARC Centre of Excellence in Plant Energy Biology
2006
The University of Western Australia
2006
Australian Research Council
2006
University of California, Los Angeles
1993-1997
Animal, Food and Health Sciences
1989-1992
Lupins are important grain legume crops that form a critical part of sustainable farming systems, reducing fertilizer use and providing disease breaks. It has basal phylogenetic position relative to other crop model legumes high speciation rate. Narrow-leafed lupin (NLL; Lupinus angustifolius L.) is gaining popularity as health food, which in protein dietary fibre but low starch gluten-free. We report the draft genome assembly (609 Mb) NLL cultivar Tanjil, captured >98% gene content,...
Mitochondria are both a source of ATP and site reactive oxygen species (ROS) production. However, there is little information on the sites mitochondrial ROS (mROS) production or biological role such mROS in plants. We provide genetic proof that complex II (Complex II) electron transport chain contributes to localized regulates plant stress defense responses. identify an Arabidopsis mutant Complex subunit, SDH1-1, through screen for mutants lacking GSTF8 gene expression response salicylic...
The analysis of plant-pathogen interactions is a rapidly moving research field and one that very important for productive agricultural systems. focus this review on the evolution plant defence responses coevolution their pathogens, primarily from molecular-genetic perspective. It explores major types including pathogen associated molecular patterns effector triggered immunity as well forces driving evolution, such mechanisms by which lineages species evolve. Advances in our understanding...
Octopine synthase (ocs) elements are a group of promoter that have been exploited by plant pathogens to express genes in plants. ocs components the promoters certain glutathione S-transferase and may function as oxidative stress response elements. Genes for element binding factors (OBFs), which belong specific class highly conserved, basic domain-leucine zipper transcription factors, isolated include Arabidopsis OBF4 OBF5 genes. To characterize proteins modulate activity OBF proteins, we...
In legumes, seed storage proteins are important for the developing seedling and an source of protein humans animals. Lupinus angustifolius (L.), also known as narrow-leaf lupin (NLL) is a grain legume crop that gaining recognition potential human health food high in dietary fibre, gluten-free low fat starch.Genes encoding NLL were characterised by sequencing cDNA clones derived from seeds. Four families identified comprised three unique α, seven β, two γ four δ conglutins. This study added...
Herbicides that mimic the natural auxin indole-3-acetic acid are widely used in weed control. One common auxin-like herbicide is dicamba, but despite its wide use, plant gene responses to dicamba have never been extensively studied. To further understand dicamba's mode of action, we utilized Arabidopsis auxin-insensitive mutants and compared their sensitivity widely-studied auxinic 2,4-dichlorophenoxyacetic (2,4-D). The mutant axr4-2, which has disrupted transport into cells, was resistant...
Rhizoctonia solani is a nectrotrophic fungal pathogen that causes billions of dollars damage to agriculture worldwide and infects broad host range including wheat, rice, potato legumes. In this study we identify wheat genes are differentially expressed in response the R. isolate, AG8, using microarray technology. A significant number identified screen were involved reactive oxygen species (ROS) production redox regulation. Levels ROS increased root tissue following infection as determined by...
Summary Overexpression of a salicylic‐acid (SA)‐inducible Arabidopsis DNA binding with one finger (Dof) transcription factor, called OBF‐binding protein 3 (OBP3; AtDof3.6), has previously been shown to result in growth defects. In this study, suppressive subtraction hybridization (SSH) was used isolate genes induced an OBP3‐overexpression line and several putative clones, OBP3‐responsive ( ORGs ), were isolated. The link the expression levels these OBP3 overexpression confirmed by analysing...
Rhizoctonia solani is an important soil-borne necrotrophic fungal pathogen, with a broad host range and little effective resistance in crop plants. Arabidopsis resistant to R. AG8 but susceptible AG2-1. A screen of 36 ecotypes mutants affected the auxin, camalexin, salicylic acid, abscisic acid ethylene/jasmonic pathways did not reveal any variation response demonstrated that was independent these defense pathways. The Affymetrix ATH1 Genome array used assess global gene expression changes...
The major proteins in lupin seeds are conglutins that have primary roles supplying carbon, sulphur and nitrogen energy for the germinating seedling. They fall into four families; α, β, γ δ. Interest these is growing as family members been shown to beneficial nutritional pharmaceutical properties. An in-depth transcriptome draft genome from narrow-leafed (NLL; Lupinus angustifolius) variety, Tanjil, were examined 16 conglutin genes identified. Using RNAseq data sets, structure expression of...
Narrow-leafed lupin (NLL; Lupinus angustifolius) is a key rotational crop for sustainable farming systems, whose grain high in protein content. It gluten-free, non-genetically modified, alternative source to soybean (Glycine max) and as such has gained interest human food ingredient. Here, we present chromosome-length reference genome the species pan-genome assembly comprising 55 NLL lines, including Australian European cultivars, breeding lines wild accessions. We core variable genes report...
Lupinus angustifolius L, also known as narrow-leafed lupin (NLL), is becoming an important grain legume crop that valuable for sustainable farming and recognised a potential human health food. Recent interest being directed at NLL to improve production, disease pest management benefits of the grain. However, studies have been hindered by lack extensive genomic resources species.A BAC library was constructed consisting 111,360 clones with average insert size 99.7 Kbp from cv Tanjil. The has...
Quinolizidine alkaloids (QAs) are toxic secondary metabolites that complicate the end use of narrow-leafed lupin (NLL; Lupinus angustifolius L.) grain, as levels sometimes exceed industry limit for its a food and feed source. The genotypic environmental influences on QA production in NLL poorly understood. Here, expression biosynthetic genes was analysed vegetative reproductive tissues bitter (high QA) sweet (low accessions. It demonstrated accessions characterized by lower gene exclusively...
We have investigated the potential use of β-conglutin protein isoforms from narrow-leafed lupin (Lupinus angustifolius L.) as a diabetes treatment.We produced purified recombinant β1-, β2-, β3-, β4-, and β6-conglutin proteins showed that β1, β3, β6 could bind to insulin. To assess modulatory effect on insulin activation meditated kinases, whole blood peripheral mononuclear cell cultures type 2 (T2D) healthy control subjects (C) were incubated with conglutin proteins. The treatment cells T2D...
Ocs‐elements are a group of related, bipartite promoter elements which have been exploited by two distinct groups plant pathogens, Agrobacterium and certain viruses to express genes in plants. The for Arabidopsis bZIP (basic region‐leucine zipper) proteins that bind ocs‐elements isolated characterized. genes, called OBF4 OBF5, were screening an genomic library with degenerate oligonucleotides complementary the DNA‐binding domains other ocs‐element‐binding proteins. OBF5 show 53% amino acid...
Ocs-elements, a family of 20 bp DNA sequences, are components number promoters active in plants. In the maize BMS cell line dominant ocs-element binding activity is transcription factor complex called OTF. The isolation cDNA clones from expression library for two bZIP (basic region-leucine zipper) proteins that bind sequence and good candidates forming at least part OTF described. proteins, OBF3.1 OBF3.2, closely related, with protein sharing 95.8% amino acid homology OBF3.2 although there...
The Arabidopsis glutathione S-transferase GSTF8 promoter directs root-specific responses to stress. In this study, the response of plant infection with Rhizoctonia solani was investigated using a luciferase reporter system. seedlings harboring GSTF8:luciferase construct were monitored in vivo for bioluminescence following R. solani. Although gene induced infected roots, differed markedly between strains and not observed aggressive that caused death seedlings. three tested detail progressed...
Crop breeding for improved disease resistance may be achieved through the manipulation of host susceptibility genes. Previously we identified multiple Arabidopsis mutants known as enhanced stress response1 (esr1) that have defects in a KH-domain RNA-binding protein and conferred increased to root fungal pathogen Fusarium oxysporum. Here, screening same mutagenized population discovered two further response also F. These leaf (Alternaria brassicicola) an aphid pest (Myzus persicae), but not...