A5033088455- Cancer therapeutics and mechanisms
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Neuroblastoma Research and Treatments
- DNA and Nucleic Acid Chemistry
- Lung Cancer Research Studies
- Neutropenia and Cancer Infections
- Chronic Lymphocytic Leukemia Research
- Advanced NMR Techniques and Applications
- Kruppel-like factors research
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Chemotherapy-induced cardiotoxicity and mitigation
- Acute Myeloid Leukemia Research
- DNA Repair Mechanisms
- Hematopoietic Stem Cell Transplantation
- RNA Research and Splicing
- Eosinophilic Disorders and Syndromes
Mustansiriyah University
2022-2025
Newcastle University
2018-2022
Abstract Anthracyclines, such as doxorubicin (adriamycin), daunorubicin, or epirubicin, rank among the most effective agents in classical anticancer chemotherapy. However, cardiotoxicity remains main limitation of their clinical use. Topoisomerase IIβ has recently been identified a plausible target anthracyclines cardiomyocytes. We examined putative topoisomerase selective agent XK469 potential cardioprotective and designed several new analogs. In our experiments, inhibited both isoforms (α...
Myeloproliferative Neoplasm (MPN) is a clonal disorder of blood progenitor cells during haematopoiesis. JAK2V617F mutation represents one the major identified genetic reasons MPN disorder. It has been shown that zygosity associated with disease phenotype. However, studies showed variable findings regarding individual phenotype parameters and significance effect. aim this study was to determine frequency corresponding outcomes in population. This cross-section recruited JAK2V617F-mutated...
The neuroblastoma cell line SH-SY5Y is widely used to study retinoic acid (RA)-induced gene expression and differentiation as a tool neurodegenerative disorders. cells predominantly exhibit adrenergic neuronal properties, but they can also exist in an epigenetically interconvertible alternative state with more mesenchymal characteristics; result, these be regulation circuitry controlling phenotype. Using combination of pharmacological inhibition targeted inactivation, we have probed the...
Abstract Genetic processes require the activity of multiple topoisomerases, essential enzymes that remove topological tension and intermolecular linkages in DNA. We have investigated subcellular localisation six human topoisomerases with a view to understanding maintenance mitochondrial Our results indicate mitochondria contain two TOP1MT TOP3A. Using molecular, genomic biochemical methods we find both proteins contribute mtDNA replication, addition decatenation role TOP3A, is stimulated by...
Natural antisense transcripts (NATs) constitute a significant group of regulatory, long noncoding RNAs. They are prominently expressed in testis but also detectable other organs. NATs transcribed at low levels and co-expressed with related protein coding sense transcripts. Nowadays generally considered as RNAs without closer focus on the inevitable interference between expression. This work describes cellular system where transcription specific locus (SLC34A1/PFN3) is induced using...
Abstract: BACKGROUND: Diagnosis of myeloproliferative neoplasms including polycythemia vera (PV) has been greatly improved following the identification driver mutations at exon 12 and 14 Janus Kinase 2 (JAK2) gene. There are limited academic studies that report frequency JAK2 gene with number patients. OBJECTIVES: The aim this study was to evaluate among JAK2V617F-negative PV-suspected Iraqi PATIENTS, MATERIALS AND METHODS: This is a retrospective conducted National Center...
Abstract BACKGROUND: Myeloproliferative neoplasms (MPNs) without Break Point Cluster- Abelson (BCR-ABL) are mainly divided into three major types: polycythemia vera, essential thrombocythemia (ET), and primary myelofibrosis (PMF). JAK2V617F-negative patients with features of MPN may need to be considered for further evaluation exclude ET diagnosis. A large proportion PMF have been shown mutations at calreticulin (CALR) gene. These mutually exclusive JAK2V617F as proposed. The aim this study...
Purpose: To date, there is no enough data about the prevalence of JAK2, CALR, and MPL mutations in BCR-ABL1 rearrangement negative myeloproliferative neoplasms (MPN) patients Iraq.This study was conducted to determine these evaluate clinical features polycythemia vera (PV) essential thrombocythemia (ET) Iraq.Methods: we evaluated presence 158 patients.JAK2V617F mutation assessed using either Allele specific PCR or quantitative PCR.JAK2 MPN were further for existence MPL, JAK2 exon 12 by...
DNA topoisomerase II beta (TOP2B) has a role in transcriptional regulation. Here, to further investigate regulation by TOP2B, we used RNA‐sequencing and real‐time PCR analyse the differential gene expression profiles of wild‐type two independent TOP2B‐null pre‐B Nalm‐6 cell lines, one generated targeted insertion other using CRISPR‐ Cas9 editing. We identified carbonyl reductase 1 ( CBR1 ) among most significantly downregulated genes these cells. Reduced was accompanied loss binding...