A5000245776- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Plant Molecular Biology Research
- RNA regulation and disease
- Ubiquitin and proteasome pathways
Baylor College of Medicine
2024
Wellcome Centre for Cell Biology
2018-2020
University of Edinburgh
2018-2020
University of Freiburg
2018
Centromeres are essential for chromosome segregation and specified epigenetically by the presence of histone H3 variant CENP-A. In flies humans, replenishment centromeric mark is uncoupled from DNA replication requires removal “placeholder” nucleosomes. Although transcription at centromeres has been previously linked to loading new CENP-A, underlying molecular mechanism remains poorly understood. Here, we used Drosophila melanogaster tissue culture cells show that actively transcribing RNA...
Abstract Replication and transcription of genomic DNA requires partial disassembly nucleosomes to allow progression polymerases. This presents both an opportunity remodel the underlying chromatin a danger losing epigenetic information. Centromeric is required for stable incorporation centromere-specific histone dCENP-A in M/G1 phase, which depends on eviction previously deposited H3/H3.3-placeholder nucleosomes. Here we demonstrate that chaperone elongation factor Spt6 spatially temporarily...
Abstract Replication and transcription of genomic DNA requires partial disassembly nucleosomes to allow progression polymerases. This constitutes both an opportunity remodel the underlying chromatin as well potential danger losing epigenetic information. Centromeric has been shown be required for stable incorporation centromere-specific histone dCENP-A in M/G1-phase, which depends on eviction previously deposited H3/H3.3-placeholder nucleosomes. Here we demonstrate that chaperone elongation...