ABSTRACT When titanium dioxide (TiO 2 ) is irradiated with near-UV light, this semiconductor exhibits strong bactericidal activity. In paper, we present the first evidence that lipid peroxidation reaction underlying mechanism of death Escherichia coli K-12 cells are in presence TiO photocatalyst. Using production malondialdehyde (MDA) as an index to assess cell membrane damage by peroxidation, observed there was exponential increase MDA, whose concentration reached 1.1 2.4 nmol · mg (dry...

To identify genes necessary for the photoproduction of H2 in Chlamydomonas reinhardtii, random insertional mutants were screened clones unable to produce H2. One identified mutants, denoted hydEF-1, is incapable assembling an active [Fe] hydrogenase. Although hydEF-1 mutant transcribes both hydrogenase and accumulates full-length protein, production activity not observed. The HydEF protein contains two unique domains that are homologous distinct prokaryotic proteins, HydE HydF, which found...

Biofuels derived from algal lipids represent an opportunity to dramatically impact the global energy demand for transportation fuels. Systems biology analyses of oleaginous algae could greatly accelerate commercialization algal-derived biofuels by elucidating key components involved in lipid productivity and leading initiation hypothesis-driven strain-improvement strategies. However, higher-level systems analyses, such as transcriptomics proteomics, are highly dependent upon available...

DNA insertional transformants of Chlamydomonas reinhardtii were screened chemochromically for attenuated H2 production. One mutant, displaying low gas photoproduction, has a nonfunctional copy gene that shows high homology to the family isoamylase genes found in several photosynthetic organisms. gel blotting and complementation used link this previously characterized nontagged sta7 mutants. This mutant is therefore denoted sta7-10. In C. reinhardtii, STA7 important accumulation crystalline...

The eukaryotic green alga, Chlamydomonas reinhardtii, produces H2 under anaerobic conditions, in a reaction catalysed by an [FeFe]-hydrogenase. To identify genes that influence production C. library of 6000 colonies on agar plates was screened with sensitive chemochromic H2-sensor films for clones defective production. Two mutants particular interest were fully characterized. One mutant, hydEF-1, is unable to assemble active This the first reported reinhardtii mutant not capable producing...

ABSTRACT When incubated in the presence of CO gas, Rubrivivax gelatinosus CBS induces a oxidation-H 2 production pathway according to stoichiometry + H O → . Once induced, this proceeds equally well both light and darkness. is not present, can serve as sole carbon source, supporting cell growth anaerobically with doubling time nearly days. This observation suggests that oxidation reaction yields energy. Indeed, new ATP synthesis was detected darkness following additions gas phase culture,...

Microalgae can make a significant contribution towards meeting global renewable energy needs in both carbon-based and hydrogen (H2) biofuel. The development of energy-related products from algae could be accelerated with improvements systems biology tools, recent advances sequencing technology provide platform for enhanced transcriptomic analyses. However, these techniques are still heavily reliant upon available genomic sequence data. Chlamydomonas moewusii is unicellular green alga capable...

ABSTRACT A hydrogenase linked to the carbon monoxide oxidation pathway in Rubrivivax gelatinosus displays tolerance O 2 . When either whole-cell or membrane-free partially purified was stirred full air (21% , 79% N ), its H evolution activity exhibited a half-life of 20 6 h, respectively, as determined by an anaerobic assay using reduced methyl viologen. atmosphere containing 3.3 13% for 15 min and evaluated hydrogen-deuterium (H-D) exchange assay, nearly 80 60% isotopic rate retained,...

Photosynthetic flavodiiron proteins catalyze oxygen reduction at non-heme iron sites (brown spheres) using flavin (FMN) mediated electron transfer (black arrows).

This Commentary reflects on the state of scholarship learning for environmental and natural resource policy governance. How have we been about learning? We highlight theoretical empirical advancements related to learning, as well areas divergence between theories frameworks, underdeveloped knowledge around processes outcomes. To address these limitations improve progress in both theory practice, offer recommendations by focusing how collectively engage ‘learning learning’.

ABSTRACT Upon exposure to carbon monoxide, the purple nonsulfur photosynthetic bacterium Rubrivivax gelatinosus produces hydrogen concomitantly with oxidation of CO according equation + H 2 O ↔ . Yet little is known about genetic elements encoding this reaction in organism. In present study, we use transposon mutagenesis and functional complementation uncover three clustered genes, cooL , cooX cooH putatively part a membrane-bound, multisubunit NiFe-hydrogenase. We complete amino acid...

Abstract Across the world, public administration and policy decisions are related to diverse levels of conflict attention. However, degree variance attention remain largely unspecified. This article examines how types energy infrastructure characteristics project location associated with distribution around siting process. Our empirical focus is on gas pipelines, electricity transmission lines, solar power projects, wind projects across United States in 2018. Primarily relying regression...

The capacity of cyanobacteria to adapt highly dynamic photon flux and nutrient availability conditions results from controlled management use reducing power, is a major contributing factor the efficiency photosynthesis in aquatic environments. response changing includes modulating gene expression protein-protein interactions that serve adjust electron mechanisms control photosynthetic transport (PET). In this regard, photochemical activity photosystem I (PSI) reaction centers can support...

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In Synechocystis sp. PCC 6803 ( S . 6803), two types of phycobilisome (PBS) complexes, CpcG-PBS and CpcL-PBS, function to harvest light energy for photosynthetic reaction centers (RCs), photosystem I (PSI) II (PSII). The compositional differences between these forms PBS their specificity RCs have led suggestions that they may differ in function. To address this question, we examined how PBS-RC interactions, the transfer excitation from RCs, might be adjusted under conditions where electron...