A5047598958- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Oral and Maxillofacial Pathology
- Protist diversity and phylogeny
- Plant nutrient uptake and metabolism
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Bone Tumor Diagnosis and Treatments
- Genomics and Phylogenetic Studies
- Tumors and Oncological Cases
- Anaerobic Digestion and Biogas Production
- Marine and coastal ecosystems
- Physiological and biochemical adaptations
- Nitrogen and Sulfur Effects on Brassica
- Head and Neck Surgical Oncology
- Medical research and treatments
- Lipid metabolism and biosynthesis
- Soft tissue tumors and treatment
- Lymphadenopathy Diagnosis and Analysis
- Reconstructive Surgery and Microvascular Techniques
- Salivary Gland Tumors Diagnosis and Treatment
- Head and Neck Cancer Studies
- Solar-Powered Water Purification Methods
- Legume Nitrogen Fixing Symbiosis
- Sarcoma Diagnosis and Treatment
University of Córdoba
2013-2025
Hospital Universitario Infanta Cristina
2010-2022
Universidad de Extremadura
2016
Centro de Implantología Cirugía Oral y Maxilofacial
2016
Carnegie Department of Plant Biology
2008-2013
Carnegie Institution for Science
2008-2013
Stanford University
2008-2010
Louisiana State University
2008
Institut de Biologie Physico-Chimique
2007
Sorbonne Université
2007
Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It model system for studying chloroplast-based photosynthesis, as well the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited common ancestor animals, but lost in plants. We sequenced ∼120-megabase nuclear genome performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated...
Abstract The Chlamydomonas reinhardtii transcriptome was characterized from nutrient-replete and sulfur-depleted wild-type snrk2.1 mutant cells. This is null for the regulatory Ser-Thr kinase SNRK2.1, which required acclimation of alga to sulfur deprivation. analyses used microarray hybridization RNA-seq technology. Quantitative RT-PCR evaluation results obtained by these techniques showed that reports a larger dynamic range expression levels than do hybridizations. Transcripts responsive...
Abstract Positive signaling by nitrate in its assimilation pathway has been studied Chlamydomonas reinhardtii. Among >34,000 lines generated plasmid insertion, 10 mutants were unable to activate reductase (NIA1) gene expression and had a Nit− (no growth nitrate) phenotype. Each of these was mutated the assimilation–specific regulatory NIT2. The complete NIT2 cDNA sequence obtained, deduced amino acid revealed GAF, Gln-rich, Leu zipper, RWP-RK domains typical transcription factors...
Abstract Chlamydomonas (Chlamydomonas reinhardtii) exhibits several responses following exposure to sulfur (S)-deprivation conditions, including an increased efficiency of import and assimilation the sulfate anion (SO4 2−). Aspects SO4 2− transport during S-replete S-depleted conditions were previously studied, although transporters had not been functionally identified. We employed a reverse genetics approach identify putative transporters, examine their regulation, establish biogenesis...
A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation thousands transformants followed by PCR-based screenings that allow identification strains harboring introduced marker gene within specific genes interest. Our results highlight strengths and limitations two independent screens differed in nature DNA used (PCR-amplified fragment containing plasmid-free versus entire linearized plasmid...
In the context of algal wastewater bioremediation, this study has identified a novel consortium formed by bacterium Methylobacterium oryzae and microalga Chlamydomonas reinhardtii that greatly increase biomass generation (1.22 g L-1·d-1), inorganic nitrogen removal (>99%), hydrogen production (33 mL·L-1) when incubated in media containing ethanol methanol. The key metabolic aspect relationship relied on bacterial oxidation to acetate, which supported heterotrophic growth. However,...
Abstract In the absence of sulfur (S), Chlamydomonas reinhardtii increases abundance several transcripts encoding proteins associated with S acquisition and assimilation, conserves amino acids, acclimates to suboptimal growth conditions. A positive regulator, SAC1 (for acclimation protein 1), a negative SAC3, were shown participate in control these processes. this study, we investigated two allelic mutants (ars11 ars44) affected gene SNRK2 SNF1-related kinase 2) designated SNRK2.1. Like sac1...
Abstract The existence of mutants at specific steps in a pathway is valuable tool functional genomics an organism. Heterologous integration occurring during transformation with selectable marker Chlamydomonas (Chlamydomonas reinhardtii) has been used to generate ordered mutant library. A strain, having chimeric construct (pNia1::arylsulfatase gene) as sensor the Nia1 gene promoter activity, was transformed plasmid bearing paramomycin resistance AphVIII insertional defective regulatory...
Currently, hydrogen fuel is derived mainly from fossil fuels, but there an increasing interest in clean and sustainable technologies for production. In this context, the ability of some photosynthetic microorganisms, particularly cyanobacteria microalgae, to produce a promising alternative renewable, clean-energy Among diverse array microorganisms able hydrogen, green algae Chlamydomonas reinhardtii model organism widely used study Despite well-known fact that acetate-containing medium...
Abstract The Chlamydomonas reinhardtii PSR1 gene is required for proper acclimation of the cells to phosphorus (P) deficiency. P-starved psr1 mutants show signs secondary sulfur (S) starvation, exemplified by synthesis extracellular arylsulfatase and accumulation transcripts encoding proteins involved in S scavenging assimilation. Epistasis analysis reveals that induction S-starvation responses P-limited requires regulatory protein kinase SNRK2.1, but bypasses membrane-targeted activator,...
We have previously shown that Prochlorococcus sp. SS120 strain takes up glucose by using a multiphasic transporter encoded the Pro1404 gene. Here we studied uptake kinetics in multiple strains from different ecotypes, observing diverse values for Ks constants (15 – 126.60 nM) and rates (0.48 6.36 pmol min-1 mg prot-1). Multiphasic was observed all strains, except TAK9803-2. gene expression studies during 21st Atlantic Meridional Transect cruise showed positive correlation with concentrations...
Abstract During sulfur (S) deprivation, the unicellular alga Chlamydomonas reinhardtii exhibits increased expression of numerous genes. These genes encode proteins associated with sulfate (SO4 2−) acquisition and assimilation, alterations in cellular metabolism, internal S recycling. Administration cytoplasmic translational inhibitor cycloheximide prevents deprivation-triggered accumulation transcripts encoding arylsulfatases (ARS), an extracellular polypeptide that may be important for cell...
A naturally occurring multispecies bacterial community composed of Bacillus cereus and two novel bacteria (Microbacterium forte sp. nov. Stenotrophomonas goyi nov.) has been identified from a contaminated culture the microalga Chlamydomonas reinhardtii. When incubated in mannitol- yeast extract-containing medium, this can promote sustain algal hydrogen production up to 313 mL H2·L−1 for 17 days 163.5 25 high-cell (76.7 μg·mL−1 initial chlorophyll) low-cell density (10 cultures, respectively....
Abstract A multispecies bacterial community including Microbacterium forte sp. nov., Stenotrophomonas goyi and Bacillus cereus greatly promoted sustained hydrogen production by the microalga Chlamydomonas reinhardtii when cocultivated in mannitol- yeast extract-containing medium (up to 313 mL·L -1 ). Alga viability was also largely prolonged cocultures (>45 days) without any nutrient supplementation. Among community, nov. main responsible for improvement. Nonetheless, use of entire...
We have isolated and characterized the Chlamydomonas reinhardtii genes for molybdenum cofactor biosynthesis, namely, CNX1G CNX1E, expressed them their chimeric fusions in Escherichia coli. In all cases, wild-type phenotype was restored individual mutants as well a CNX1E double mutant. Therefore, CrCNX1E is first eukaryotic protein able to complement an E. coli moeA
ABSTRACT Microbacterium fakhimi sp. nov. has been isolated from a contaminated algal culture ( Chlamydomonas reinhardtii ). Its genome fully sequenced (3,753,259 base pairs) and tentative annotation is provided (3,704 genes). Both, information growth tests suggest that M. auxotroph for biotin thiamine unable to use sulfate as sulfur (S) source. S-reduced forms, such methionine cysteine can support growth. The potential biotechnological interest of this bacteria discussed here in related...