A5032962048- Mitochondrial Function and Pathology
- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- Algal biology and biofuel production
- Metabolism and Genetic Disorders
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- Photoreceptor and optogenetics research
- Marine and coastal ecosystems
- Microbial Metabolic Engineering and Bioproduction
- Protein Structure and Dynamics
- Diatoms and Algae Research
- Fungal and yeast genetics research
- Pancreatic function and diabetes
- Genetics and Neurodevelopmental Disorders
- Marine and coastal plant biology
- Adipose Tissue and Metabolism
- Plant and soil sciences
- GABA and Rice Research
- Plant nutrient uptake and metabolism
- Cassava research and cyanide
- Health and Medical Education
- Trypanosoma species research and implications
- RNA and protein synthesis mechanisms
Universidad Nacional Autónoma de México
2015-2025
Instituto Nacional de Cardiología
1996-2022
University of Liège
2005-2016
The Ohio State University
2009
Institut de Biologie Physico-Chimique
2007
Joint Genome Institute
2007
Sorbonne Université
2007
Centre National de la Recherche Scientifique
2007
Duke University
2007
University of Massachusetts Chan Medical School
2007
Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It model system for studying chloroplast-based photosynthesis, as well the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited common ancestor animals, but lost in plants. We sequenced ∼120-megabase nuclear genome performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated...
Apicomplexan parasites, including the human pathogens Toxoplasma and Plasmodium , contain a vestigial plastid, apicoplast. This chloroplast-derived organelle is remnant of secondary endosymbiosis between an ancestral apicomplexan photosynthetic organism whose origin moot ([1–4][
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSubunit arrangement in beef heart complex IIIDiego Gonzalez-Halphen, Margaret A. Lindorfer, and Roderick CapaldiCite this: Biochemistry 1988, 27, 18, 7021–7031Publication Date (Print):September 6, 1988Publication History Published online1 May 2002Published inissue 6 September 1988https://pubs.acs.org/doi/10.1021/bi00418a053https://doi.org/10.1021/bi00418a053research-articleACS PublicationsRequest reuse permissionsArticle...
Abstract Pure mitochondria of the photosynthetic algaChlamydomonas reinhardtii were analyzed using blue native-polyacrylamide gel electrophoresis (BN-PAGE). The major oxidative phosphorylation complexes resolved: F1F0-ATP synthase, NADH-ubiquinone oxidoreductase, ubiquinol-cytochrome c reductase, and cytochrome oxidase. oligomeric states these determined. synthase runs exclusively as a dimer, in contrast to C. chloroplast enzyme, which is present monomer subcomplexes. sequence 60-kD protein,...
The mitochondrial genomes of Chlamydomonad algae lack the cox2 gene that encodes essential subunit COX II cytochrome c oxidase. is normally a single polypeptide encoded by gene. In this work we cloned two nuclear genes encoding from both Chlamydomonas reinhardtii and Polytomella sp. cox2agene protein, IIA, corresponding to N-terminal portion oxidase, thecox2b IIB, C-terminal region. cox2a cox2b are located in nucleus independently transcribed into mRNAs translated separate polypeptides....
The atp6 gene, encoding the ATP6 subunit of F(1)F(0)-ATP synthase, has thus far been found only as an mtDNA-encoded gene. However, is absent from mtDNAs some species, including that Chlamydomonas reinhardtii. Analysis C. reinhardtii expressed sequence tags revealed three overlapping sequences encoded a protein with similarity to proteins. PCR and 5'- 3'-RACE were used obtain complete cDNA genomic atp6. gene exhibited characteristics nucleus-encoded gene: Southern hybridization signals...
A novel mitochondrial DNA (mtDNA) mutation at position nt 4320 in the tRNA(Ile) gene was associated with severe encephalopathy a 7-month-old infant, who died of intractable hypertrophic cardiomyopathy. The present heteroplasmic fashion (88%) muscle and fulfills accepted criteria for pathogenicity. This is fourth pathogenic identified this gene, which appears to be "hotspot" deleterious mutations affecting heart. report adds evidence genetic heterogeneity cardiomyopathies.
In yeast, mammals, and land plants, mitochondrial F1FO-ATP synthase (complex V) is a remarkable enzymatic machinery that comprises about 15 conserved subunits. Peculiar among eukaryotes, complex V from Chlamydomonadales algae (order of chlorophycean class) has an atypical subunit composition its peripheral stator dimerization module, with nine subunits unknown evolutionary origin (Asa subunits). vitro, this enzyme exhibits increased stability dimeric form, in vivo, Chlamydomonas reinhardtii...
The majority of our knowledge about mitochondrial genomes Viridiplantae comes from land plants, but much less is known their green algal relatives. In the order Sphaeropleales (Chlorophyta), only one representative genome currently available—that Acutodesmus obliquus. Our study adds nine completely sequenced and three partially spanning phylogenetic diversity Sphaeropleales. We show not a size range 25–53 kb variation in intron content (0–11) gene also conservation 13 core respiratory genes...
The algae of the family Chlamydomonadaceae lack gene <i>cox3</i> that encodes subunit III cytochrome <i>c</i> oxidase in their mitochondrial genomes. This observation has raised question whether this is present or corresponding located nucleus. Cytochrome was isolated from colorless chlamydomonad <i>Polytomella</i> spp., and existence established by immunoblotting analysis with an antibody directed against <i>Saccharomyces cerevisiae</i>subunit III. Based partly upon N-terminal sequence...
Purification of the glutamate synthase (GOGAT) enzyme from Saccharomyces cerevisiae showed that it is an oligomeric composed three identical 199-kDa subunits. The GOGAT structural gene was isolated by screening a yeast genomic library with PCR probe. This probe obtained amplification degenerate oligonucleotides designed conserved regions known genes. derived amino-terminal sequence confirmed direct analysis purified protein 199 kDa. Northern (RNA) allowed identification mRNA about 7 or 8 kb....
The homologous polypeptides in yeast and beef heart cytochrome c oxidase have been identified by sequence comparisons structural similarities. properties of individual used to specify which components are extrinsic, intrinsic bilayer spanning, the complex.