A5084147559- Chromium effects and bioremediation
- Metabolomics and Mass Spectrometry Studies
- Enzyme Structure and Function
- Metabolism and Genetic Disorders
- Alcohol Consumption and Health Effects
- Microbial Metabolic Engineering and Bioproduction
- Pancreatic function and diabetes
- Biofuel production and bioconversion
- Protein Structure and Dynamics
- Trace Elements in Health
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Microbial Community Ecology and Physiology
- Metabolism, Diabetes, and Cancer
- Iron Metabolism and Disorders
- Fungal and yeast genetics research
- Amino Acid Enzymes and Metabolism
- Enzyme-mediated dye degradation
- Folate and B Vitamins Research
- Air Quality and Health Impacts
- Arsenic contamination and mitigation
- Fermentation and Sensory Analysis
- Nitric Oxide and Endothelin Effects
- Bacterial Genetics and Biotechnology
- Air Quality Monitoring and Forecasting
- Protein Kinase Regulation and GTPase Signaling
Universidad Nacional Autónoma de México
2014-2024
Universidad Autónoma de la Ciudad de México
2017-2019
Wilfrid Laurier University
2019
Instituto Nacional de Cardiología
2003
Instituto de Ecología
1997
Summary NADPH oxidases (Nox) have been characterized as higher eukaryotic enzymes used deliberately to produce reactive oxygen species (ROS). The recent discovery of new functional members the Nox family in plants and animals has led recognition increasing importance ROS signals involved regulation diverse cellular processes such defence, growth signalling. Here, we address role oxidase‐generated biology filamentous fungus Aspergillus nidulans . We characterize noxA gene show that it encodes...
A comprehensive, structural and functional, in silico analysis of the medium‐chain dehydrogenase/reductase (MDR) superfamily, including 583 proteins, was carried out by use extensive database mining blastp program an iterative manner to identify all known members superfamily. Based on phylogenetic, sequence, functional similarities, protein MDR superfamily were classified into three different taxonomic categories: (a) subfamilies, consisting a closed group containing set ideally orthologous...
Mass extinctions have disrupted the evolutionary history of several species, and with it discovery novel proteins biomedical biotechnological value. However, Limulus polyphemus, a xiphosuran marine arthropod, is "living fossil" 480-million-year-old lineage. To explore this legacy, rMe'exLec1, 13.15 kDa tandem-repeat lectin from L. was studied. Named after ancient Maya term "Me'ex" ("spider fish"), Identified in genome, recombinantly expressed Escherichia coli, purified via nickel affinity...
ChrA is a membrane protein that confers resistance to the toxic ion chromate through energy‐dependent efflux from cytoplasm. In databases, member of transporter (CHR) superfamily, composed at least several dozens members, distributed in three domains life. The aim this work was perform phylogenetic analysis CHR superfamily. An exhaustive search for homologous proteins carried out National Center Biotechnology Information database. One hundred and thirty‐five sequences were identified as...
Inosine, an endogenous nucleoside, has recently been shown to exert potent effects on the immune, neural, and cardiovascular systems. This work addresses modulation of intermediary metabolism by inosine through adenosine receptors (ARs) in isolated rat hepatocytes. We conducted silico search GenBank complete genomic sequence databases for additional adenosine/inosine a feasible physiological role homeostasis. Inosine stimulated glycogenolysis (≈40%, EC 50 4.2 × 10 −9 M), gluconeogenesis 7.8...
Catecholamines in adipose tissue promote lipolysis via cAMP, whereas insulin stimulates lipogenesis. Here we show that H(2)O(2) generated by rat adipocytes impaired cAMP-mediated amplification cascade of lipolysis. These micromolar concentrations added before cAMP suppressed activation type IIbeta cyclic AMP-dependent protein kinase (PKA) holoenzyme, prevented hormone-sensitive lipase translocation from cytosol to storage droplets, and inhibited Similarly, IIalpha PKA holoenzyme bovine heart...
Background Alcohol dehydrogenase (ADH) activity is widely distributed in the three domains of life. Currently, there are non-homologous NAD(P)+-dependent ADH families reported: Type I comprises Zn-dependent ADHs; type II short-chain ADHs described first Drosophila; and, III iron-containing (FeADHs). These arose independently throughout evolution and possess different structures mechanisms reaction. While types have been extensively studied, analyses about diversity (type III) FeADHs not...
Non-steroidal anti-inflammatory drugs (NSAIDs) —aspirin, naproxen, nimesulide, and piroxicam— lowered activation of type II cAMP-dependent protein kinase A (PKA-II) in isolated rat adipocytes, decreasing adrenaline- dibutyryl cAMP (Bt2cAMP)-stimulated lipolysis. The molecular bases insulin-like actions NSAID were studied. Based on the reported inhibition lipolysis by H2O2, catalase was successfully used to block inhibitory action Bt2cAMP-stimulated NSAID, at (sub)micromolar range, induced an...
Oxidative stress is a factor that contributes to the development of complications in diabetes; however, its effects can be counteracted using exogenous antioxidants are found some plants, which why people turn traditional medicines search for therapeutic treatment. Justicia spicigera has been demonstrated have capacity reduce glycemic levels; on non-insulin-dependent organs such as liver not reported. During 30 days administration ethanol extract, blood glucose and weight rats were measured...
K+ dependence was assumed to be a feature of all pyruvate kinases until it discovered that some enzymes express -independent activity. Almost the have Lys at position 117, instead Glu present in -dependent muscle enzyme. Mutagenesis studies show internal positive charge substitutes for requirement (Laughlin, L. T. & Reed, G. H. (1997) Arch. Biochem. Biophys. 348, 262-267). In this work phylogenetic analysis kinase performed ascertain abundance activities and explore whether activating effect...
Tandem paired genes encoding putative short-chain monodomain protein members of the chromate ion transporter (CHR) superfamily (ywrB and ywrA) were cloned from genomic DNA Bacillus subtilis strain 168. The transcription genes, renamed chr3N chr3C, respectively, was shown to occur via a bicistronic mRNA generated promoter upstream gene. chr3C conferred resistance when expressed in Escherichia coli W3110. gene alone did not confer on E. coli, suggesting that both are required for function....
Plant ALDH10 enzymes are aminoaldehyde dehydrogenases (AMADHs) that oxidize different ω-amino or trimethylammonium aldehydes, but only some of them have betaine aldehyde dehydrogenase (BADH) activity and produce the osmoprotectant glycine (GB). The latter possess alanine cysteine at position 441 (numbering spinach enzyme, SoBADH), while those ALDH10s cannot (BAL) isoleucine this position. Only plants contain A441- C441-type isoenzymes accumulate GB in response to osmotic stress. In work we...