A5066373860- Crystallization and Solubility Studies
- Mycotoxins in Agriculture and Food
- X-ray Diffraction in Crystallography
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Metal complexes synthesis and properties
- Venomous Animal Envenomation and Studies
- Receptor Mechanisms and Signaling
- Fungal Biology and Applications
- Antimicrobial Peptides and Activities
- Protein Tyrosine Phosphatases
- Lipid Membrane Structure and Behavior
- Biochemical and Structural Characterization
- Invertebrate Immune Response Mechanisms
- Carcinogens and Genotoxicity Assessment
- Glycosylation and Glycoproteins Research
- Immune Response and Inflammation
- Allergic Rhinitis and Sensitization
- Studies on Chitinases and Chitosanases
- Estrogen and related hormone effects
- Food Allergy and Anaphylaxis Research
- Toxin Mechanisms and Immunotoxins
- Galectins and Cancer Biology
- Natural product bioactivities and synthesis
- Enzyme Production and Characterization
Universidad Nacional Autónoma de México
2016-2025
Universidad Autónoma de la Ciudad de México
2014-2024
Toxalim Research Centre in Food Toxicology
2011-2018
Université de Montréal
2017-2018
College of Staten Island
2006-2010
CHRISTUS Spohn Hospital Corpus Christi - Memorial
2010
City University of New York
2006-2010
Wageningen University & Research
2010
University of Pittsburgh
2004
Michigan State University
2004
Peroxisome proliferator-activated receptor α (PPARα) is a nuclear expressed in tissues with high oxidative activity that plays central role metabolism. In this work, we investigated the effect of hepatocyte PPARα on non-alcoholic fatty liver disease (NAFLD).We constructed novel hepatocyte-specific knockout (Pparα(hep-/-)) mouse model. Using model, performed transcriptomic analysis following fenofibrate treatment. Next, which physiological challenges impact PPARα. Moreover, measured...
The androgen testosterone is essential for the Sertoli cell to support maturation of male germ cells and production spermatozoa (spermatogenesis). In classical view action, binding intracellular receptor (AR) produces a conformational change in AR such that receptor–steroid complex has high affinity specific DNA regulatory elements able stimulate gene transcription. Here, we demonstrate can act by means an alternative, rapid, sustainable mechanism independent AR–DNA interactions....
Severe neutrophilic asthma is poorly responsive to glucocorticosteroids (GC). Neutrophil extracellular traps (NETs) within the lungs have been associated with severity of airway obstruction and inflammation in asthma, were found be unaffected by GC vitro. As IL-17 overexpressed contributes steroid insensitivity different cell types, we hypothesized that NETs formation asthmatic airways would resistant through an mediated pathway. Six severe horses six healthy controls studied while being...
Abstract Antimicrobial resistance is emerging as a global health challenge that requires immediate and concerted attention. Accordingly, the WHO has issued alerts urging to continue developing antibiotics with novel mechanisms of action toward clinically important pathogens, including Acinetobacter baumannii . In this context, fungi have played crucial role in discovery development antibiotics. Therefore, work, three fungal strains were prioritized based on their metabolic profiles...
Mass extinctions have disrupted the evolutionary history of several species, and with it discovery novel proteins biomedical biotechnological value. However, Limulus polyphemus, a xiphosuran marine arthropod, is "living fossil" 480-million-year-old lineage. To explore this legacy, rMe'exLec1, 13.15 kDa tandem-repeat lectin from L. was studied. Named after ancient Maya term "Me'ex" ("spider fish"), Identified in genome, recombinantly expressed Escherichia coli, purified via nickel affinity...
Abstract Tissue recombination experiments show that prostate mesenchyma directs epithelial cell growth and development in an androgen-dependent manner, functional differentiation of epithelium requires androgen-driven processes both epithelia stroma. The androgen induction target genes primary cultures stromal cells was determined using adenoviral expression system, which employed the MMTV-enhancer driven luciferase reporter as receptor (AR)–mediated transcription assay. These studies...
Plants express chitinase and chitinase-like proteins (CLPs) belonging to the glycosyl hydrolases of GH18 GH19 families, which exhibit varied functions. CLPs in family have been structurally functionally characterized; however, there are no structures available for any member family. In this study, two from rubber tree Hevea brasiliensis (HbCLP1 HbCLP2) were cloned, expressed characterized. HbCLP1 was identical allergen Hev b 11.0101 previously described by others, while HbCLP2 a novel...
Organotin complexes were prepared through a one-pot reaction with three components by reacting thiosemicarbazide or 4-methyl-3-thiosemicarbazide 4-phenylthiosemicarbazide, dehydroacetic acid (DHA) and dibutyl, diphenyl, dicyclohexyl, bis[(trimethylsilyl)methyl]tin(IV) oxides; all characterized infrared (IR), ultraviolet-visible (UV-vis), mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy. The 119Sn NMR revealed chemical shifts corresponding to pentacoordinated environment...
Abstract Oligomerization of allergens plays an important role in IgE-mediated reactions, as effective crosslinking IgE- FcεRI complexes on the cell membrane is dependent number exposed B-cell epitopes a single allergen molecule or occurrence identical symmetrical arrangement. Few studies have attempted to experimentally demonstrate connection between dimerization and ability trigger allergic reactions. Here we studied plant allergenic profilins rHev b 8 (rubber tree) rZea m 12 (maize)...
Wheat germ agglutinin (WGA) demonstrates potential as an oral delivery agent owing to its selective binding carbohydrates and capacity traverse biological membranes. In this study, we employed differential scanning calorimetry molecular dynamics simulations comprehensively characterize the thermal unfolding process of both complete lectin four isolated domains. Furthermore, present nuclear magnetic resonance structures three domains that were previously lacking experimental in their forms....
Vibrio parahaemolyticus (Vp) is the etiological agent of acute hepatopancreatic necrosis disease (AHPND) in Penaeus vannamei shrimp. Vp possesses a 63-70 kb conjugative plasmid that encodes binary toxin PirAvp/PirBvp. The 250 kDa PirABvp complex was purified by affinity chromatography with galactose-sepharose 4B and on stroma from glutaraldehyde-fixed rat erythrocytes column, as heterotetramer PirAvp PirBvp subunits. In addition, recombinant pirB (rPirBvp) pirA (rPirAvp) were obtained....
Abstract Allergies have become a rising health problem, where plentiful substances can trigger IgE-mediated allergies in humans. While profilins are considered minor allergens, these ubiquitous proteins primary molecules involved cross-reactivity and pollen-food allergy syndrome. Here we report the first crystal structures of murine Fab/IgE, with its chains naturally paired, complex allergen profilin from Hevea brasiliensis (Hev b 8). The crystallographic models revealed that IgE’s six...
Abstract The presence of serotonin 1A receptor (5‐HT ‐R) in the hippocampus, amygdala, and most regions frontal cortex is essential between postnatal day‐5–21 (P5–21) for expression normal anxiety levels adult mice. Thus, 5‐HT ‐R plays a crucial role this time window brain development. We show that ‐R‐mediated stimulation extracellular signal‐regulated kinases 1 2 (Erk1/2) hippocampus undergoes transition P6 P15. At P6, protein kinase C (PKC) isozyme required ⇒Erk1/2 cascade, which causes...
The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins insect preys. In this respect, spiders as a diversified group of almost exclusive predators appear to possess infinite potential discovery novel insect-selective toxins. 2003, was isolated from Macrothele gigas and amino acid sequence reported. We obtained, by molecular biology techniques in heterologous system, one these Purification process...
Plant chitinases are enzymes that have several functions, including providing protection against pathogens. Agave tequilana is an economically important plant poorly studied. Here, we identified a chitinase from short reads of the A. transcriptome (AtChi1). A second chitinase, differing by only six residues first, was isolated total RNA plants infected with Fusarium oxysporum (AtChi2). Both were overexpressed in Escherichia coli and analysis their sequences indicated they belong to class I...