Ankylosing spondylitis (AS) is a chronic inflammatory disease of unknown etiology, though it considered an autoimmune disease. HLA-B27 the risk factor most often associated with AS, and although mechanism involvement unclear, subtypes other features relationship between AS have been studied for years. Additionally, key role IL-17 Th17 cells in autoimmunity inflammation suggests that latter cytokines involved their generation could play pathogenesis this Recent studies described sources...

Acute hepatopancreatic necrosis disease (AHPND) was first reported in China 2009 and afterwards Mexico 2013. AHPND is caused by Vibrio parahaemolyticus affects Penaeus monodon Litopenaeus vannamei shrimp cultures. The bacterium contains the pirA- pirB-like genes 69- to 70-Kb plasmids, which encode toxins that produce disease. aim of this study determine whether existed bacterial genera distinct from before cases were documented Mexico. Two isolates selected farms Nayarit 2006 analysed...

Vibrio parahaemolyticus (Vp) is the etiological agent of acute hepatopancreatic necrosis disease (AHPND) in Penaeus vannamei shrimp. Vp possesses a 63-70 kb conjugative plasmid that encodes binary toxin PirAvp/PirBvp. The 250 kDa PirABvp complex was purified by affinity chromatography with galactose-sepharose 4B and on stroma from glutaraldehyde-fixed rat erythrocytes column, as heterotetramer PirAvp PirBvp subunits. In addition, recombinant pirB (rPirBvp) pirA (rPirAvp) were obtained....

Liver cirrhosis (LC) is an inflammatory process associated with impaired functions in adaptive and innate immune responses at both systemic local levels, also referred as Cirrhosis-Associated Immune Dysfunction. In this study, we evaluated the functionality of neutrophils from ascitic fluid (AF) patients hepatic by testing their ability to generate neutrophil extracellular traps (NETs) vitro. To further determine activation state neutrophils, expression markers CD66b, CD69, CD80 on these...

White spot syndrome virus (WSSV) has a ∼300 kb double-stranded DNA genome. It originated in China, spread rapidly through shrimp farms Asia, and subsequently to America. This study determined complete genome sequences for nine historic WSSV strains isolated from Pacific white (Litopenaeus vannamei) captured farm ponds northwest Mexico (Sinaloa Nayarit). Genomic was by an amplification method using overlapping long-range PCR sequencing Ion Torrent-PGM. Complete were assembled (length range...

To test whether effluents released from white spot syndrome virus (WSSV)-infected farm ponds are a pathway for spreading WSSV, WSSV-negative Pacific whiteleg shrimp, Litopenaeus vannamei, were exposed to WSSV-containing water under conditions of fluctuating temperatures. White disease outbreaks occurred at the shrimp before and during experiment. Two cages placed inside each pond, one was outlet canal. Each cage stocked with 30 shrimp. Hemolymph collected intervals 24, 48, 72, 120, 168, 360...

White spot disease (WSD) causes high mortality in cultured shrimp throughout the world. Its etiologic agent is white syndrome virus (WSSV). The genomic repeat regions ORF 75, 94, and 125 have been used to classify WSSV isolates epidemiological studies using PCR with specific primers sequencing. present study investigated variation nucleotide sequences from 107, 150, 143 of collected Litopenaeus vannamei ponds WSD outbreaks northwestern Mexico during period 2010-2012, ORFs 125, respectively....

A Taenia solium glutathione-S-transferase fraction (SGSTF) was isolated from a metacestode crude extract by affinity chromatography on reduced glutathione (GSH)-sepharose. The purified displayed specific S-transferase (GST) activity of 2.8 micromol/min/mg and peroxidase selenium-independent 0.22 micromol/min/mg. Enzymatic characterization the suggested that closer to mammalian mu-class GSTs. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, filtration, enzyme analysis showed...

Gnathostomiasis is an emerging systemic parasitic disease acquired by consuming raw or uncooked fresh-water fish infected with the advanced third-stage larvae of Gnathostoma spp. This endemic to Pacific region Mexico, and one its etiologic agents has been identified as binucleatum . We characterized humoral immune response patients clinically diagnosed gnathostomiasis detecting total IgM, IgE, IgG class subclasses against a crude extract parasite Western blotting. Our results do not show...

Abstract Aim Ankylosing spondylitis (AS) pathogenesis has focused on the adaptive immune response; however, innate responses may also play a role in inflammatory response of AS. Dysregulated neutrophil activation can induce tissue damage and contribute to immune‐related diseases. Hence, aim this study was assess effect complexes formed with p30 Salmonella typhimurium anti‐p30 antibodies present sera AS patients controls inducing release extracellular traps (NETs) secretion pro‐inflammatory...

Objective. To determine the association of Salmonella typhimurium antigens with AS by analysing IgA, IgG and IgM antibody response to crude lysate 30-kDa band from this micro-organism. Methods. Sera 28 patients, HLA-B27+ healthy relatives, unrelated subjects 14 RA patients were included. proteins electrophoretically separated blotted onto nitrocellulose sheets for immunodetection sera subjects. The electroeluted (p30) a lysat (StCL) S. used as antigen evaluate IgM, IgA (total subclasses)...

This study explored whether Crassostrea gigas oysters can be used as a bioindicator of white spot syndrome virus (WSSV) in shrimp farm water canals. Bioassays showed that C. accumulate WSSV their gills and digestive glands but do not become infected, either by exposure to seawater containing or cohabitation with infected shrimp. The use nested PCR screen placed canals at the entry allowed detected 16 d prior disease occurring. finding concentrate small amounts present without being harmed...

The <i>IKZF1 </i>gene is formed by 8 exons and encodes IKAROS, a transcription factor that regulates the expression of genes control cell cycle progression survival. In general, 15-20% patients with preB acute lymphoblastic leukemia (preB ALL) harbor <i>IKZF1</i> deletions, frequency these deletions increases in <i>BCR-ABL1</i> or Ph-like subgroups. These have been associated poor treatment response risk relapse. aim this descriptive study was to determine...

Objectives: To determine the lymphoproliferative response to 30-kDa protein (p30) of Salmonella typhimurium in patients with ankylosing spondylitis (AS).Method: Lymphoproliferative was determined peripheral blood mononuclear cells (PBMCs) from 30 AS and 40 healthy subjects. Cells were cultured concanavalin A (Con A), a crude lysate S. (StCL), or p30. Lymphoproliferation measured by MTT assay.Results: Our data show that mitogenic Con similar both groups studied; however, StCL p30...

Summary Gnathostomiasis is a prevalent zoonosis in humans some regions of the world. The genus Gnathostoma considered an accidental parasite for humans; G. binucleatum endemic species Nayarit, Mexico. This work was designed to determine proteolytic activity excretory-secretory products (ESP) advanced third-stage larvae (ADVL3) against human fibronectin and antibodies from sheep. Our findings showed protease as well sheep gamma globulins ESP at molecular weights 80 56 kDa. proteases found are...

Rheumatoid arthritis (RA) is a chronic inflammatory disease whose association with SNPs has led to the identification of biomarkers in different populations. To determine -857C/T SNP TNFA gene RA and clinical parameters, 233 patients 237 healthy controls were included this study. The polymorphism was determined using TaqMan® system features also determined. We found that Hardy-Weinberg equilibrium. Our results showed no RA; however, carrying TT genotype lower anti-CCP levels than other...

Abstract Rheumatoid arthritis (RA) is an autoimmune disease characterized by inflammatory process that affects mainly synovial tissue in joints, and the production of cyclic citrullinated peptides (anti-CCP) antibodies. In regulation nuclear factor kappa B (NFkB) transcription activation a key point cytokines. On other hand, polymorphisms several genes could contribute to promotion observed RA, association rs28362491 polymorphism NFkB gene with RA has been studied different population....