A5085072058- Neuroscience and Neuropharmacology Research
- Animal Disease Management and Epidemiology
- Ion channel regulation and function
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Immunology Research
- Receptor Mechanisms and Signaling
- Cardiac electrophysiology and arrhythmias
- Molecular Sensors and Ion Detection
- Insect Resistance and Genetics
- Lipid Membrane Structure and Behavior
- Virology and Viral Diseases
- Vector-Borne Animal Diseases
- Nicotinic Acetylcholine Receptors Study
- Animal Virus Infections Studies
- Transgenic Plants and Applications
- Photoreceptor and optogenetics research
- Chemical Synthesis and Analysis
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Historical and Environmental Studies
- Virus-based gene therapy research
- Plant tissue culture and regeneration
- Venomous Animal Envenomation and Studies
- Invertebrate Immune Response Mechanisms
- Viral Infections and Vectors
The University of Texas Health Science Center at Houston
2019-2025
Universidad Nacional Autónoma de México
2013-2021
Instituto de Agrobiotecnología del Litoral
2019
National Agricultural Technology Institute
2000-2017
Centro Científico Tecnológico - San Juan
2017
Consejo Nacional de Investigaciones Científicas y Técnicas
2002-2015
Universidad Nacional de Colombia
2015
Medicina
2013
National Institute of Industrial Technology
2000-2011
Centro Científico Tecnológico - Tucumán
2010
A large-scale vaccination experiment involving a total of 138 cattle was carried out to evaluate the potential synthetic peptides as vaccines against foot-and-mouth disease. Four types representing sequences disease virus (FMDV) C3 Argentina 85 were tested: A, which includes G-H loop capsid protein VP1 (site A); AT, in T-cell epitope has been added site A; AC, composed and carboxy-terminal region C); ACT, three previous motifs are colinearly represented. Induction neutralizing antibodies,...
Abstract N 6 -methyladenosine (m6A) is the most prevalent internal RNA modification that can impact mRNA expression post-transcriptionally. Recent progress indicates m6A also acts on nuclear or chromatin-associated RNAs to transcriptional and epigenetic processes. However, landscapes functional roles of in human brains neurodegenerative diseases, including Alzheimer’s disease (AD), have been under-explored. Here, we examined methylome using total RNA-seq meRIP-seq middle frontal cortex...
Ionotropic glutamate receptors (iGluRs) are tetrameric ligand-gated ion channels that mediate most excitatory neurotransmission1. iGluRs gated by glutamate, where on binding, they open their to enable cation influx into postsynaptic neurons, initiating signal transduction1,2. The structural mechanics of how gating occurs in full-length is not well understood. Here, using the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid subtype iGluR (AMPAR), we identify glutamate-gating mechanism....
Allostery can be manifested as a combination of repression and activation in multidomain proteins allowing for fine tuning regulatory mechanisms. Here we have used single molecule fluorescence resonance energy transfer (smFRET) molecular dynamics simulations to study the mechanism allostery underlying negative cooperativity between two agonists glutamate glycine NMDA receptor. These data show that binding one agonist leads conformational flexibility an increase spread at second site....
Delta receptors are members of the ionotropic glutamate receptor superfamily and form trans-synaptic connections by interacting with extracellular scaffolding protein cerebellin-1 presynaptic transmembrane neurexin-1β. Unlike other family members, however, direct agonist-gated ion channel activity has not been recorded in delta receptors. Here, we show that GluD2 subtype forms cation-selective channels when bound to Using fluorescence lifetime measurements chemical cross-linking, reveal...
Fast excitatory synaptic transmission in the mammalian central nervous system is mediated by glutamate-activated α-amino-5-methyl-3-hydroxy-4-isoxazole propionate (AMPA) receptors. In neurons, AMPA receptors coassemble with transmembrane receptor regulatory proteins (TARPs). Assembly TARP γ8 alters biophysical properties of receptor, producing resensitization currents continued presence glutamate. Using single-channel recordings, we show that under resensitizing conditions, GluA2 primarily...
Abstract Memantine is an US Food and Drug Administration (FDA) approved drug that selectively inhibits NMDA-subtype ionotropic glutamate receptors (NMDARs) for treatment of dementia Alzheimer’s. NMDARs enable calcium influx into neurons are critical normal brain function. However, increasing evidence shows in neurological diseases augmented by calcium-permeable AMPA-subtype (AMPARs). Here, we demonstrate these AMPARs (CP-AMPARs) inhibited memantine. Electrophysiology unveils memantine...
N-methyl-D-aspartate (NMDA) receptors are ionotropic glutamate involved in learning and memory. NMDA primarily comprise two GluN1 GluN2 subunits. The subunit dictates biophysical receptor properties, including the extent of activation desensitization. GluN2A- GluN2D-containing represent functional extremes. To uncover conformational basis their divergence, we utilize single-molecule fluorescence resonance energy transfer to probe extracellular domains these subtypes under resting...
With persistent foot-and-mouth disease virus (FMDV) in BHK-21 cells, there is coevolution of the cells and resident virus; virulence for parental gradually increased, become partially resistant to FMDV. Here we report that variants FMDV C3Arg/85 were selected a single infection (termed BHK-Rb cells). Indirect immunofluorescence showed cell population was heterogeneous with regard susceptibility infection. Infection resulted an early phase partial cytopathology which followed at 6 10 days...
Evidence for a mechanism of initiation viral persistence in which the cell, and not virus, plays critical role has been obtained using important animal pathogen foot-and-mouth disease virus (FMDV). We have developed virulence assay consisting quantification ability to kill cells divide presence initiate carrier state. Cells were cured FMDV at early times following cytolytic infection BHK-21 monolayers with FMDV. When subjected they showed an increased survive second by but other RNA viruses....
In this work we analyze the antigenic properties and stability in cell culture of virus mutants recovered upon challenge peptide-vaccinated cattle with foot-and-mouth disease (FMDV) C3 Arg85. Previously, showed that a significant proportion 29 lesions analyzed (41%) contained viruses single amino acid replacements (R141G, L144P, or L147P) within major site located at G-H loop VP1, known to participate also interactions integrin receptors. Here document no were found from 12 developed six...
Newcastle disease virus (NDV) is the causative agent of an economically important disease, which affects all species birds worldwide. Current vaccination programs for NDV include use either low-virulent live-virus vaccines or inactivated to induce protective immunity while producing minimal adverse effects in birds. In order further characterize immune response elicited by live and conventional chickens, we evaluated presence specific antibodies different secretions tissue culture...
Zn 2+ has been shown to have a wide range of modulatory effects on neuronal AMPARs. However, the mechanism modulation is largely unknown. Here we show that inhibits GluA2(Q) homomeric receptors in an activity- and voltage-dependent manner, indicating pore block mechanism. The rate inhibition slow, hundreds milliseconds at millimolar concentrations; hence, only observed residual nondesensitizing currents. Consequently, higher for GluA2 complex with auxiliary subunits γ2 γ8 where activation...
Foot-and-mouth disease virus (FMDV) shows a remarkable antigenic variability and, like other RNA viruses, presents high rate of mutation. It has been proposed that selection exerted by antibodies the host could play major role in rapid evolution FMDV. The present work reports FMDV antibody-resistant (Nr) populations after serial passages cloned O1 Caseros strain on secondary monolayers bovine kidney cells presence subneutralizing antiviral polyclonal sera (APS). After limited number...
Wild waterfowl is considered a natural reservoir of potentially infectious agents and source pathogenic viruses like avian paramyxoviruses type 1 (APMV 1). In 1997, commercial poultry in Argentina had reached the status being free from virulent Newcastle disease virus (NDV) infections. Vaccination biosecurity measures are actively performed to maintain this preferential sanitary condition. However, risk reintroduction always present. context, we conducted study describe wild healthy birds...