A5050244787- Protein Structure and Dynamics
- Enzyme Structure and Function
- Proteins in Food Systems
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Enzyme Production and Characterization
- Protein Interaction Studies and Fluorescence Analysis
- Enzyme Catalysis and Immobilization
- Lipid Membrane Structure and Behavior
- Microencapsulation and Drying Processes
- Protein Hydrolysis and Bioactive Peptides
- Biochemical and Structural Characterization
- Toxin Mechanisms and Immunotoxins
- RNA and protein synthesis mechanisms
- Electrochemical sensors and biosensors
- Diatoms and Algae Research
- thermodynamics and calorimetric analyses
- Carbohydrate Chemistry and Synthesis
- Microbial Inactivation Methods
- Chemical Synthesis and Analysis
- Natural product bioactivities and synthesis
- Spectroscopy and Quantum Chemical Studies
- Protease and Inhibitor Mechanisms
- Calcium Carbonate Crystallization and Inhibition
- Pineapple and bromelain studies
Universidad Autónoma Metropolitana
2012-2024
Universidad Nacional Autónoma de México
2004
A commercial preparation of laccase (EC 1.10.3.2), cloned from Myceliophthora thermophila and expressed in Aspergillus oryzae (MtL), was purified modified by conjugation with poly(ethylene glycol) (Mr = 5000) is labeled PEG−MtL. Native enzyme found to have a molecular mass 80 kDa, as determined gel filtration, 110 SDS−PAGE. The oxidative dimerization 2,6-dimethoxyphenol (DMP) produce the corresponding dibenzoquinone catalyzed MtL manner comparable that for diffusion-controlled reaction...
In triosephosphate isomerase, Cys126 is a conserved residue located close to the catalytic glutamate, Glu165. Although it has been mentioned that and other nearby residues are required maintain active site geometry optimal for catalysis, no evidence supporting this idea reported date. work, we studied stability properties of mutants C126A C126S Saccharomyces cerevisiae TIM (wtTIM). None these amino acid replacements induced significant changes in folding wtTIM, as indicated by spectroscopic...
The thermal denaturation of the dimeric enzyme triosephosphate isomerase (TIM) from Saccharomyces cerevisiae was studied by spectroscopic and calorimetric methods. At low protein concentration structural transition proved to be reversible in scannings conducted at a rate greater than 1.0 °C min-1. Under these conditions, however, denaturation−renaturation cycle exhibited marked hysteresis. use lower scanning rates lead pronounced irreversibility. Kinetic studies indicated that likely...
Wheat germ agglutinin (WGA) demonstrates potential as an oral delivery agent owing to its selective binding carbohydrates and capacity traverse biological membranes. In this study, we employed differential scanning calorimetry molecular dynamics simulations comprehensively characterize the thermal unfolding process of both complete lectin four isolated domains. Furthermore, present nuclear magnetic resonance structures three domains that were previously lacking experimental in their forms....
Abstract We have made a comparative structure based analysis of the thermodynamics lectin‐carbohydrate (L‐C) binding and protein folding. Examination total change in accessible surface area those processes revealed much larger decrease free energy per unit buried case L‐C associations. According to our analysis, this stabilization interactions arises from more favorable enthalpy burying polar area, higher proportions areas. Hydrogen bonds present at 14 interfaces were identified, their...
Two forms of stem bromelain (EC 3.4.22.4) were isolated from commercial, crude and chromatographically purified preparations the enzyme by means gel-filtration cation-exchange liquid chromatography. These possess nearly identical secondary tertiary structures, as judged their circular dichroism (c.d.) spectra. The spectral characteristics suggest that this belongs to alpha + beta protein class, other cysteine proteinases do. In agreement with these results, quantitative estimation structures...
The thermal denaturation of the acid proteinase from Aspergillus saitoi was studied by CD and differential scanning calorimetry (DSC). This process seemed to be completely irreversible, as protein samples that were heated temperatures at which transition had been completed then cooled 25 degrees C did not show any reversal change in signal. Similar results obtained with DSC. Nevertheless, we able detect presence reversibly unfolded species experiments enzyme solution a temperature within...
Isothermal titration calorimetry was used to characterize thermodynamically the association of hevein, a lectin from rubber tree latex, with dimer and trimer N-acetylglucosamine (GlcNAc). Considering changes in polar apolar accessible surface areas due complex formation, we found that experimental binding heat capacities can be reproduced adequately by means parameters protein-unfolding studies. The same conclusion applies concanavalin A methyl-α-mannopyranoside. When reduced area change,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDifferential scanning calorimetry of the irreversible denaturation Escherichia coli glucosamine-6-phosphate deaminaseAndres Hernandez-Arana, Arturo Rojo-Dominguez, Myriam M. Altamirano, and Mario L. CalcagnoCite this: Biochemistry 1993, 32, 14, 3644–3648Publication Date (Print):April 13, 1993Publication History Published online1 May 2002Published inissue 13 April...
Factors affecting TiO<sub>2</sub> biomineralization using TiBALDH as precursor.
We report the heptapeptide-mediated biomineralization of titanium dioxide nanoparticles from alkoxides. evaluated influence pH on biomineralized products and found that nanostructured TiO2 was formed in absence external ions (water only) at ∼ 6.5. Several variants (mutants) peptides with different properties (i.e., charges, isoelectric points (pIs), sequences) were designed tested experiments. Acid-catalyzed experiments run using H1 (HKKPSKS) peptide room temperature, which produced anatase...
A global census of stereochemical metrics including interface size, hydropathy, amino acid propensities, packing and hydrogen bonding was carried out on 32 x-ray-elucidated structures lectin–carbohydrate complexes covering eight different lectin families. It is shown that the interactions at primary binding subsites are more efficient than other subsites. Another salient behavior found for a marked negative correlation between size polar surface content. noteworthy this demographic rule...