A5089558647- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrochemical Analysis and Applications
- Radical Photochemical Reactions
- Lipid metabolism and biosynthesis
- Copper Interconnects and Reliability
- Synthesis and Catalytic Reactions
- Ion-surface interactions and analysis
- Chemical Synthesis and Analysis
- Microfluidic and Capillary Electrophoresis Applications
- Metabolism and Genetic Disorders
Texas A&M University
2021-2025
Mitchell Institute
2022-2023
Combinatorial electrochemistry has great promise for accelerated reaction screening, organic synthesis, and catalysis. Recently, we described a new high-throughput platform, colloquially named "Legion". Legion fits the footprint of 96-well microtiter plate with simultaneous individual control over all 96 electrochemical cells. Here, demonstrate versatility when coupled mass spectrometry (MS) electrosynthetic product screening quantitation. Electrosynthesis benzophenone azine was selected as...
Abstract Knowing concentrations of lipids is essential for understanding their physiological functions and discovering new disease biomarkers. However, it highly challenging to accurately quantify due structural diversity multiple isomeric forms lipids. To address these critical gaps, we have developed a novel aziridine‐based isobaric tag labelling strategy that allows (i) determination lipid double‐bond positional isomers, (ii) accurate relative quantification unsaturated lipids, (iii)...
Characterization of nonpolar lipids is crucial due to their essential biological functions and ability exist in various isomeric forms. In this study, we introduce the N-H aziridination method target carbon-carbon double bonds (C═C bonds) sterol lipids. The resulting fragments are readily dissociated upon collision-induced dissociation, generating specific fragment ions for C═C bond position determination fingerprint backbone characterization. This significantly enhances lipid ionization...
Abstract Knowing concentrations of lipids is essential for understanding their physiological functions and discovering new disease biomarkers. However, it highly challenging to accurately quantify due structural diversity multiple isomeric forms lipids. To address these critical gaps, we have developed a novel aziridine‐based isobaric tag labelling strategy that allows (i) determination lipid double‐bond positional isomers, (ii) accurate relative quantification unsaturated lipids, (iii)...
Characterization of glycerophospholipid isomers is significant importance as they play different roles in physiological and pathological processes. In this work, we present a novel bifunctional derivatization method utilizing Mn(II)-catalyzed epoxidation to simultaneously identify carbon-carbon double bond (CC bond)- stereonumbering (sn)-positional phosphatidylcholine. Mn(II) coordinates with picolinic acid catalyzes unsaturated lipids by peracetic acid. Collision-induced dissociation (CID)...
Lipids are important biomarkers within the field of disease diagnostics and can serve as indicators progression predictors treatment effectiveness. Although lipids provide insight into how diseases initiate progress, mass spectrometric methods for lipid characterization profiling limited due to structural diversity, particularly presence various isomers. Moreover, difficulty handling small-volume samples exacerbates intricacies biological analyses. In this work, we have developed a strategy...
Characterization of nonpolar lipids is significance, as they serve a variety key biological functions and can naturally exist in isomeric forms. Electrospray ionization mass spectrometry (ESI-MS) powerful tool for most lipid analysis, but do not easily ionize electrospray, complicating their analyses. In this work, we use the Du Bois catalyst (Rh2(esp)2) aziridina-tion carbon-carbon double bonds (C=C bond) six sterol lipids, simultaneously increasing efficiency facilitating C=C bond...
Microdroplets have recently emerged as a unique form of chemical reactor that has gained significant attention due to the interfacial chemistry produces dramatic reaction acceleration. In this work, we developed strategy allows thin film be delivered directly interface by electromigration in theta capillary. Additional acceleration was observed reactants being transferred electro-oxidative C-H/N-H coupling phenothiazine with N, N’-dimethylaniline, and short-lived intermediates were captured...
Unsaturated lipids constitute a dominant portion of in mammalian cells and often exist as mixture isomers. Knowing concentrations is essential for understanding their functions physiology diseases. Herein, novel aziridination-based isobaric tag labeling strategy developed isomeric characterization accurate quantification unsaturated lipids. This allows (i) determination lipid double bond positional isomers, (ii) (iii) improvement ionization efficiencies nonpolar The power the method has been...