A5066327885- Ophthalmology and Visual Impairment Studies
- Corneal surgery and disorders
- Glaucoma and retinal disorders
- DNA Repair Mechanisms
- Retinal and Optic Conditions
- Retinal Development and Disorders
- Ocular Diseases and Behçet’s Syndrome
- Rock Mechanics and Modeling
- Fluorine in Organic Chemistry
- CRISPR and Genetic Engineering
- Carcinogens and Genotoxicity Assessment
- Fungal and yeast genetics research
- Retinal and Macular Surgery
- Radical Photochemical Reactions
- Retinal Diseases and Treatments
- Receptor Mechanisms and Signaling
- Cerebral Venous Sinus Thrombosis
- Geophysical Methods and Applications
- Ocular Infections and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Grouting, Rheology, and Soil Mechanics
- Visual perception and processing mechanisms
- Long-Term Effects of COVID-19
- X-ray Diffraction in Crystallography
- Biochemical Analysis and Sensing Techniques
First Affiliated Hospital Zhejiang University
2020-2025
China University of Mining and Technology
2023-2024
Zhejiang University
2016-2024
Hebei University
2023-2024
University of Science and Technology Beijing
2024
Heilongjiang University
2023
Tangshan Normal University
2022-2023
Wuhan University of Technology
2018-2022
Southwest Jiaotong University
2022
Beijing University of Agriculture
2018
Abstract Objective This study aimed to assess the presence of novel coronavirus in tears and conjunctival secretions SARS–CoV‐2‐infected patients. Methods A prospective interventional case series was performed, 30 confirmed pneumonia (NCP) patients were selected at First Affiliated Hospital Zhejiang University from 26 January 2020 9 February 2020. At an interval 2 3 days, tear collected twice with disposable sampling swabs for reverse‐transcription polymerase chain reaction (RT‐PCR) assay....
The effectiveness of <i>in vitro</i> primer elongation reactions catalyzed by human bypass DNA polymerases κ (hDinB1), pol η (hRad30A), ι (hRad30B), and yeast ζ (Rev3 Rev7) in site-specifically modified template oligonucleotide strands were studied vitro</i>. templates contained single bulky lesions derived from the <i>trans</i>-addition mutagenic (+)- or (−)-enantiomers of<i>r</i>7, <i>t</i>8-dihydroxy-<i>t</i>9,10-epoxy-7,8,9,10-tetrahydrobenzo[<i>a</i>]pyrene (a metabolite environmental...
Since the outbreak of Coronavirus Disease 2019 (COVID-19) in December 2019, many studies have reported presence severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) conjunctival sac patients infected with this virus, several displaying symptoms viral conjunctivitis. However, to our best knowledge, there is no in-depth report on course COVID-19 complicated by relapsing conjunctivitis or keratoconjunctivitis.A 53-year-old man confirmed developed left eye approximately 10 days after...
AIM: To compare analysis of the impact high altitude on corneal endothelial cells, and prognosis in patients with cataract surgeries. METHODS: Totally 265 plateau surgeries performed between January 2019 July 2022 (average altitude=3000 m), 524 plain 2020 were included. The propensity score matching (PSM) method was applied to match basic information both regions a 1:1 basis. Corneal cell density (ECD), coefficient variation (CV), hexagonal ratio (HEX), duration surgery, pre- postoperative...
DNA polymerase μ (Polμ) is a newly discovered member of the X family with unknown cellular function. The understanding Polμ function should be facilitated by an its biochemical activities. By using purified human for analyses, we lesion bypass activities this in response to several types damage. When it encountered template 8-oxoguanine, abasic site, or 1,N 6-ethenoadenine, efficiently bypassed lesion. Even bulky adducts such asN-2-acetylaminofluorene-adducted guanine, (+)- and...
Abstract The mechanisms underlying myopia remain not fully understood. We proposed to examine the function and of miR-204-5p in development. expression level was assessed vitreous humor (VH) a cohort consisting 11 patients with high (HM) 16 control undergoing vitrectomy. Then functional implications ARPE-19 cells were assessed. Thioredoxin-interacting protein (TXNIP) found as possible target through mRNA sequencing, its interaction confirmed employing luciferase assay western blotting....
Benzo[a]pyrene is a potent environmental carcinogen, which can be metabolized in cells to the DNA damaging agent anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (anti-BPDE). We hypothesize that mutations induced by BPDE adducts are mainly generated through an error-prone translesion synthesis requires specialized polymerase (Pol). Using vivo mutagenesis assay yeast model system, we have examined potential roles of Polζ and Polη (±)-anti-BPDE-induced mutagenesis. In proficient mutagenesis,...
Changes in the retina and choroid blood vessels are regularly observed myopia. However, if retinal glial cells, which directly contact vessels, play a role mammalian myopia is unknown. We aimed to explore potential mechanism of cells form deprived myopia.We adapted mice form-deprivation model by covering right eye left open for control, measured ocular structure with anterior segment optical coherence tomography, evaluated changes morphology distribution fluorescence staining western...
Significance Tyrosyl-DNA phosphodiesterase 1 (TDP1) is a novel repair enzyme that removes 3′-DNA blocking groups generated by topoisomerase cleavage complexes, oxidative DNA lesions, and base alkylation during normal transcription replication, widely used anticancer drugs induce strand breaks including inhibitors (topotecan, irinotecan, etoposide), bleomycin, alkylating agents (temozolomide). This report demonstrates the Drosophila gene glaikit (gkt) ortholog of TDP1. It also critical...
A metal-free electrochemical oxidative difluoroethylation of 2-arylbenzimidazoles was accomplished, which provided an efficient strategy for the synthesis MeCF2-containing benzo[4,5]imidazo[2,1-a]-isoquinolin-6(5H)-ones. In addition, method also enabled construction various difluoroethylated indolo[2,1-a]isoquinolin-6(5H) ones. Notably, this protocol proceeded well under mild conditions without metal catalysts or exogenous additives/oxidants added.
The Drosophila insulin receptor (DInR) regulates a diverse array of biological processes including growth, axon guidance, and sugar homeostasis. Growth regulation by DInR is mediated Chico, the homolog vertebrate insulin-receptor-substrate proteins IRS1-4. In contrast, photoreceptor guidance in developing visual system SH2-SH3 domain adaptor protein Dreadlocks (Dock). vitro studies others identified five NPXY motifs, one juxtamembrane region four signaling C-terminal tail (C-tail), important...
Abstract Reducing the width of safe coal pillar near geological structures, such as a water-conducting fault, to improve extraction ratio seam is major concern that needs be addressed urgently for mining shallow resources in China. To tackle contradiction between improving and disaster caused by reducing waterproof pillar, this study focuses on retaining (rock) fault proposes novel method calculating waterproofing precisely. We consider different location relationships confined aquifer seam,...