A5089375927- Neurological disorders and treatments
- Conducting polymers and applications
- Single-cell and spatial transcriptomics
- Alzheimer's disease research and treatments
- Advanced biosensing and bioanalysis techniques
- Parkinson's Disease Mechanisms and Treatments
- Ubiquitin and proteasome pathways
- Heat shock proteins research
- 14-3-3 protein interactions
- ATP Synthase and ATPases Research
- Advanced Fluorescence Microscopy Techniques
- Genetics and Neurodevelopmental Disorders
University of Twente
2022-2024
Theoretical concepts from polymer physics are often used to describe intrinsically disordered proteins (IDPs). However, amino acid interactions within and between regions of the protein can lead deviations typical scaling behavior even short-lived secondary structures. To investigate key in dynamic IDP α-synuclein (αS) at level, we conducted single-molecule fluorescence resonance energy transfer (smFRET) experiments coarse-grained molecular dynamics (CG-MD) simulations. We find excellent...
The aggregation of α-synuclein (αS) plays a key role in Parkinson's disease (PD) etiology. While the onset PD is age-related, cellular quality control system appears to regulate αS throughout most human life. Intriguingly, protein 14-3-3τ has been demonstrated delay and various models. However, molecular mechanisms behind this remain elusive. Our study confirms by 14-3-3τ, unveiling concentration-dependent relation. Utilizing microscale thermophoresis (MST) single-molecule burst analysis, we...
Abstract Neurodegenerative diseases are characterised by the progressive loss of neuronal tissue, and accumulation amyloid fibrils. Currently, there no therapeutics that remove these amyloids. Targeted protein degradation could be a promising strategy to fibrils or oligomeric precursors. This approach requires degraders specifically recognise fibrils, preferentially in early stages. Here, we introduce FibrilPaint20 (FP20), peptide mediates ubiquitination It acts as PROTAC, containing both...
Genetically encoded visible fluorescent proteins (VFPs) are a key tool used to visualize cellular processes. However, compared synthetic fluorophores, VFPs photophysically complex. This photophysical complexity includes the presence of non-emitting, dark within ensemble VFPs. Quantitative fluorescence microcopy approaches that rely on obtain molecular insights hampered by these proteins. To account for proteins, it is necessary know fraction (fdark) in ensemble. date, fdark has rarely been...