ADVERTISEMENT RETURN TO ISSUEPREVComment/ReplyNEXTComment on "Measurement and Correlation of Solubilities (Z)-2-(2-Aminothiazol-4-yl)-2-methoxyiminoacetic Acid in Different Pure Solvents Binary Mixtures Water + (Ethanol, Methanol, or Glycol)"Abolghasem Jouyban*†, Mohammad A. Fakhree‡, William Eugene Acree, Jr.§View Author Information† Drug Applied Research Center Faculty Pharmacy, Tabriz University Medical Sciences, 51664, Iran‡ Tuberculosis Lung Disease Center, Iran§ Department Chemistry,...

First cases that point at a correlation between SARS-CoV-2 infections and the development of Parkinson's disease (PD) have been reported. Currently, it is unclear if there also direct causal link these diseases. To obtain first insights into possible molecular relation viral aggregation α-synuclein protein amyloid fibrils characteristic for PD, we investigated effect presence proteins on aggregation. We show, in test tube experiments, spike (S-protein) has no aggregation, while nucleocapsid...

Biomolecular condensates present in cells can fundamentally affect the aggregation of amyloidogenic proteins and play a role regulation this process. While liquid-liquid phase separation by themselves act as an alternative nucleation pathway, interaction partly disordered aggregation-prone with preexisting that localization centers could be far more general mechanism altering their behavior. Here, we show so-called host biomolecular both accelerate slow down amyloid formation. We study...

Cytotoxicity of the futuristic nanogenomedicine (e.g., short interfering RNA and antisense) may hamper its clinical development. Of these, gene-based medicine and/or carrier elicit cellular toxicity. For assessment such cytotoxicity, a common methodology is largely dependent upon utilization 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay which has been widely used as colorimetric approach based on activity mitochondrial dehydrogenase enzymes in cells. In this...

The intrinsically disordered protein α-synuclein (αS) is thought to play an important role in cellular membrane processes. Although vitro experiments indicate that this initially obtains structure upon binding, NMR and EPR studies cells could not single out any conformational subensemble. Here we microinjected small amounts of αS, labeled with a Förster resonance energy transfer (FRET) pair, into SH-SY5Y investigate changes binding. Our FRET show clear difference between αS the cytosol when...

Solubility of 2-hydroxybenzoic acid (salicylic acid, with measured melting point 432 K) in water, 1-propanol, 2-propanol, and 2-propanone was determined at (298.2 to 338.2) K atmospheric pressure. Also, the solubility salicylic binary mixtures 1-propanol (1) + water (2), 2-propanol (2) 298.2 pressure investigated. Phase separation occurred x1= 0.114, 0.167 0.171, 0.237 mixtures. The van't Hoff Grant equations were used correlate monosolvents different temperatures. values solvents calculated...

Alpha-synuclein (αS) is an intrinsically disordered protein (IDP) that abundantly present in the brain and associated with Parkinson's disease (PD). In spite of its abundance contribution to PD pathogenesis, exact cellular function αS remains largely unknown. The ability remodel phospholipid model membranes combined biochemical studies suggests involved endocytosis. To unravel which route(s) stage(s) endocytic pathway associated, we quantified colocalization between marker proteins...

Experimental solubilities of three antiepileptic drugs, that is, lamotrigine, diazepam, and clonazepam in ethanol + water mixtures at 298.15 K were reported. The solubility drugs was increased with the addition ethanol, reached maximum values, then decreased further increase concentrations. Jouyban−Acree model fitted to data, reproduced using two previously developed relationships employing a number solvent's solute's parameters data monosolvents which overall mean deviations (OMDs) models...

The solubilities of phenothiazine in water, ethanol, and propylene glycol were measured at (298.2 to 338.2) K. Also, the solubility binary mixtures ethanol + glycol, ternary mixture water was investigated. van't Hoff equation used correlate monosolvents different temperatures. values solvents calculated using Jouyban–Acree model (Jouyban, A.; Acree, W. E., Jr. J. Chem. Eng. Data2009, 54, 1168–1170). mean deviation as an error criterion. overall correlated data temperatures mixed 298.2 K 2.8...

Although it is well established that the protein α-synuclein (αS) plays an important role in Parkinson's disease, its physiological function remains largely unknown. It has been reported to bind membranes and play a membrane remodeling processes. The mechanism by which αS remodels still debated; may either affect physical properties or act as chaperone for other associated proteins. To obtain insight into of we investigated number proteins with single small vesicles neuronal cell model....

Theoretical concepts from polymer physics are often used to describe intrinsically disordered proteins (IDPs). However, amino acid interactions within and between regions of the protein can lead deviations typical scaling behavior even short-lived secondary structures. To investigate key in dynamic IDP α-synuclein (αS) at level, we conducted single-molecule fluorescence resonance energy transfer (smFRET) experiments coarse-grained molecular dynamics (CG-MD) simulations. We find excellent...

Abstract First cases that point at a correlation between SARS-CoV-2 infections and the development of Parkinson’s disease have been reported. Currently it is unclear if there also direct causal link these diseases. To obtain first insights into possible molecular relation viral aggregation α-synuclein protein amyloid fibrils characteristic for disease, we investigated effect presence proteins on aggregation. We show, in test tube experiments, S-protein has no while N-protein considerably...

Excess molar volumes and partial were investigated from density measurements for glycerol formal + water mixtures at temperatures 278.15 to 313.15 K. are fitted using Redlich–Kister equation compared with literature values other systems. The system exhibits negative excess volumes, probably due increased interactions like hydrogen bonding or large differences in of components. effect temperature on different volumetric properties studied is also analysed. Besides, the volume thermal...

Cytotoxicity of the futuristic nanogenomedicine (e.g., short interfering RNA and antisense) may hamper its clinical development. Of these, gene-based medicine and/or carrier elicit cellular toxicity. For assessment such cytotoxicity, a common methodology is largely dependent upon utilization 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay which has been widely used as colorimetric approach based on activity mitochondrial dehydrogenase enzymes in cells. In this...