A5052830153- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Viral Infectious Diseases and Gene Expression in Insects
German Cancer Research Center
2019-2020
Heidelberg University
2019-2020
Article21 July 2020Open Access Transparent process Disentangling molecular mechanisms regulating sensitization of interferon alpha signal transduction Frédérique Kok orcid.org/0000-0002-7855-1422 Division Systems Biology Signal Transduction, German Cancer Research Center (DKFZ), Heidelberg, Germany Faculty Biosciences, Heidelberg University, Search for more papers by this author Marcus Rosenblatt Institute Physics, University Freiburg, FDM - Freiburg Data Analysis and Modeling, Melissa...
The induction of an interferon-mediated response is the first line defense against pathogens such as viruses. Yet, dynamics and extent interferon alpha (IFNα)-induced antiviral genes vary remarkably comprise three expression clusters: early, intermediate late. By mathematical modeling based on time-resolved quantitative data, we identified mRNA stability well a negative regulatory loop key mechanisms endogenously controlling IFNα-induced in hepatocytes. Guided by model, uncovered that this...
Abstract The induction of an interferon-mediated response is the first line defense against pathogens such as viruses. Yet, dynamics and extent interferon alpha (IFNα)-induced antiviral genes vary remarkably comprise three expression clusters: early, intermediate late. By mathematical modeling based on time-resolved quantitative data, we identified mRNA stability well a negative regulatory loop key mechanisms endogenously controlling IFNα-induced in hepatocytes. Guided by model, uncovered...
Cellular signaling cascades adapt to changing environments through multiple feedback mechanisms. Pre-activation of the IFNα signal-transduction pathway can cause a desensitized state in which system is not responding further treatment constitutes serious therapeutic problem. Even though involvement USP18 as one key negative regulators has been reported, underlying mechanisms well role components remain unresolved.
SOP for thawing, cultivation and freezing of the Huh7.5 cell line by Division Systems Biology Signal Transduction, German Cancer Research Center (DKFZ).