A5008069376- Genetics, Aging, and Longevity in Model Organisms
- Congenital heart defects research
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Circadian rhythm and melatonin
- Digestive system and related health
- Renal and related cancers
- Developmental Biology and Gene Regulation
- Respiratory Support and Mechanisms
- Parasitic Diseases Research and Treatment
- Diabetes Management and Research
- Vibrio bacteria research studies
- Reproductive Biology and Fertility
- Muscle Physiology and Disorders
- Diabetes and associated disorders
- Genetics and Neurodevelopmental Disorders
- Physiological and biochemical adaptations
- Legionella and Acanthamoeba research
- Evolution and Genetic Dynamics
- Tissue Engineering and Regenerative Medicine
- S100 Proteins and Annexins
- Mesenchymal stem cell research
- Wnt/β-catenin signaling in development and cancer
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
University of Manitoba
2015-2023
Health Sciences Centre
2023
Children's Hospital Research Institute of Manitoba
2023
University of Pennsylvania
2011-2013
University of Calgary
2005-2012
Fox Chase Cancer Center
2007-2011
University of Cincinnati
2009-2011
Alberta Children's Hospital
2010
Institut Pasteur
2008
Transcriptionally silent genes can be marked by histone modifications and regulatory proteins that indicate the genes' potential to activated. Such marks have been identified in pluripotent cells, but it is unknown how such occur descendant, multipotent embryonic cells restricted cell fate choices. We isolated mouse endoderm assessed at elements of are activated upon liver or pancreas found distinct chromatin patterns. Furthermore, acetyltransferase P300, recruited via bone morphogenetic...
Pax3/7-dependent stem cells play an essential role in skeletal muscle development. We now show that Fgfr4 lies genetically downstream from Pax3 and is a direct target. In chromatin immunoprecipitation (ChIP)-on-chip experiments, binds to sequence 3′ of the gene directs Pax3-dependent expression at sites myogenesis transgenic mouse embryos. The activity this regulatory element also partially dependent on E-boxes, targets myogenic factors, which are expressed as progenitor enter program. Other...
The critical pancreatic transcription factor Pdx1 is expressed throughout the pancreas early but enriched in insulin-producing β cells postnatally. Previous studies showed that 5′ conserved promoter regions areas I and II (Pdx1PB) direct endocrine cell expression, while an adjacent region (Pdx1XB) containing area III directs transient β-cell expression. In this study, we used Cre-mediated lineage tracing to track activated these regions. Pdx1PBCre mediated only recombination, Pdx1XBCre...
Understanding how silent genes can be competent for activation provides insight into development as well cellular reprogramming and pathogenesis. We performed genomic location analysis of the pioneer transcription factor FoxA in adult mouse liver found that about one-third bound sites are near genes, including without detectable RNA polymerase II. Virtually all FoxA-bound within conserved sequences, suggesting possible function. Such enriched motifs transcriptional repressors, Rfx1 type II...
The paired-box homeodomain transcription factor Pax3 is a key regulator of the nervous system, neural crest and skeletal muscle development. Despite important role this factor, very few direct target genes have been characterized. We show that Itm2a, which encodes type 2 transmembrane protein, in vivo, by combining genetic approaches vivo chromatin immunoprecipitation assays. generated conditional mutant allele for an imprinted gene, flanking exons 2–4 with loxP sites inserting IRESnLacZ...
Foxi1e is a zygotic transcription factor that essential for the expression of early ectodermal genes. It expressed in highly specific pattern, only deep cell layers animal hemisphere, and mosaic pattern which expressing cells are interspersed with non-expressing cells. Previous work has shown several signals blastula control this including nodals, TGFβ family member Vg1, Notch. However, these all inhibitory, raises question what activates Foxi1e. In work, we show related Forkhead protein,...
Legionella pneumophila, a causative agent of Legionnaires' disease, is facultative intracellular parasite freshwater protozoa. pneumophila features unique developmental network that involves several forms including the infectious cyst forms. Reservoirs L. include natural and man-made systems; however, recent studies have shown isolates can also be obtained directly from garden potting soil suggesting presence an additional reservoir. A previous study employing metazoan Caenorhabditis...
Thoracic surgeries involving resection of lung tissue pose a risk severe postoperative pulmonary complications, including acute respiratory distress syndrome (ARDS) and failure. Lung resections require one-lung ventilation (OLV) and, thus, are at higher ventilator-induced injury (VILI) attributable to barotrauma volutrauma in the one ventilated lung, as well hypoxemia reperfusion on operated lung. Further, we also aimed assess differences localized systemic markers injury/inflammation those...