- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Marine Biology and Ecology Research
- Protist diversity and phylogeny
- Horticultural and Viticultural Research
- Forest Insect Ecology and Management
- Methane Hydrates and Related Phenomena
- Ocean Acidification Effects and Responses
- Plant Surface Properties and Treatments
- Marine Biology and Environmental Chemistry
- Isotope Analysis in Ecology
- Powdery Mildew Fungal Diseases
- Marine Bivalve and Aquaculture Studies
- Muscle Physiology and Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Yeasts and Rust Fungi Studies
- Atmospheric chemistry and aerosols
- Plant Pathogens and Fungal Diseases
- Marine and coastal plant biology
- Marine Ecology and Invasive Species
- Microplastics and Plastic Pollution
- Bioenergy crop production and management
- Coral and Marine Ecosystems Studies
- Insect Pheromone Research and Control
- Insect behavior and control techniques
Institut Méditerranéen d’Océanologie
2013-2023
Aix-Marseille Université
2013-2023
Institut de Recherche pour le Développement
2013-2023
Centre National de la Recherche Scientifique
2013-2023
Université de Toulon
2014-2023
Station Marine d'Endoume
2011-2018
Centre d’Immunologie de Marseille-Luminy
2011-2012
Institut de Neurobiologie de la Méditerranée
2012
Deleted Institution
2003
Nitrogen is essential for life but often a major limiting nutrient growth in the ocean. Biological dinitrogen fixation source of new nitrogen to surface waters and promotes marine productivity. Yet fate diazotroph-derived (DDN) ecosystems has been poorly studied, its transfer auto- heterotrophic plankton not measured. Here, we use high-resolution nanometer scale secondary ion mass spectrometry (nanoSIMS) coupled with 15N2 isotopic labelling flow cytometry cell sorting examine DDN specific...
Amyotrophic lateral sclerosis (ALS) is characterized by progressive denervation leading to muscle atrophy prevented, during the early phase, compensatory reinnervation. Little known about fibre regeneration capacity in ALS. We have carried out vivo and vitro investigation of skeletal Seven ALS patients underwent a deltoid biopsy. Immunohistochemical analysis revealed various degrees denervation- reinnervation-related changes biopsies including satellite cells (SCs) activation regenerating...
Stable isotope ratios of six size fractions zooplankton (80 to .2000mm) were analyzed seasonally in 2009-2010 at the SOMLIT site Bay Marseille.Isotopic generally increased with size.The highest d 15 N values observed 1000 -2000 mm fraction.The largest class (.2000 mm), dominated by gelatinous plankton, had lower due low isotopic signatures most these organisms.In larger (.1000 measured taxon level.Brachyuran, stomatopod, teleost and cephalopod larvae showed values, salps pteropods lowest...
When immersed in seawater, substrates are rapidly colonized by both micro- and macroorganisms. This process is responsible for important economic ecological prejudices, particularly when related to ship hulls or aquaculture nets. Commercial antifouling coatings supposed reduce biofouling, i.e., macrofoulers. In this study, biofilms that primarily settled on seven different (polyvinyl chloride [PVC], a fouling release coating [FRC], five self-polishing copolymer [SPC], including four...
Age-related sarcopenia could partly result from cumulative repeated episodes of incomplete repair and regeneration. We hypothesized that mitotic metabolic events associated with satellite cell activation proliferation be altered aging. Muscle-derived cells (mdc) were isolated gastrocnemius quadriceps muscles young (3 wk old), adult (9 mo old (24 old) Sprague-Dawley male rats ( n = 10/group). The mdc growing started to proliferate earlier compared animals. Cell cycle duration was...
Abstract. Biological dinitrogen (N2) fixation is the major source of new nitrogen (N) for open ocean, and thus promotes marine productivity, in particular vast N-depleted regions surface ocean. Yet, fate diazotroph-derived N (DDN) ecosystems poorly understood, its transfer to auto- heterotrophic surrounding plankton communities rarely measured due technical limitations. Moreover, different diazotrophs involved N2 (Trichodesmium spp. vs. UCYN) exhibit distinct patterns inhabit ecological...
Here, we combined flow cytometry (FCM) and phylogenetic analyses after cell sorting to characterize the dominant groups of prokaryotic assemblages inhabiting two ponds increasing salinity: a crystallizer pond (TS) with salinity 390 g/L, non-crystallizer (M1) 200 g/L retrieved from solar saltern Sfax in Tunisia. As expected, FCM analysis enabled resolution high nucleic acid content (HNA) low (LNA) prokaryotes. Next, performed taxonomic bacterial archaeal communities comprising most populated...
Abstract. Biological dinitrogen (N2) fixation provides the major source of new nitrogen (N) to open ocean, contributing more than atmospheric deposition and riverine inputs N supply. Yet fate diazotroph-derived (DDN) in planktonic food web is poorly understood. The main goals this study were (i) quantify how much DDN released dissolved pool during N2 transferred bacteria, phytoplankton zooplankton, (ii) compare release transfer efficiencies under contrasting activity diversity oligotrophic...
Diazotrophs are often limited by iron (Fe) availability in the oligotrophic ocean. The Western Tropical South Pacific (WTSP) ocean has been suggested as an intense N2 fixation area due to Fe fertilizations through shallow hydrothermal activity. Yet, demand of diazotrophs their natural habitat, where they cohabit with other microbial organisms also requiring Fe, remains unknown. Here we develop and apply a method consisting coupling 55Fe uptake experiments cell-sorting flow cytometry, provide...
After the exponential growth phase, variability in scattering efficiency of phytoplankton cells over their complete life cycle is not well characterised. Bulk measurements are impacted by senescent and detritrus. Thus analysis evolution optical properties thanks to morphological and/or intra-cellular variations remains poorly studied. Using Cytosense flow cytometer (CytoBuoy b.v., NL), temporal course forward sideward efficiencies two species (Thalassiosira pseudonana Chlamydomonas...
In the dark ocean, balance between heterotrophic carbon demand and supply of sinking through biological pump remains poorly constrained. situ tracking dynamics microbial degradation processes occurring on gravitational particles is still challenging. Our particle simulator system (PASS) intends to mimic as closely possible in variations pressure temperature experienced by particles. Here, we used PASS simultaneously track geochemical changes that occurred during mesopelagic zone...
Abstract. The distribution of ultraphytoplankton was investigated in the western North Pacific Subtropical Gyre (NPSG) during La Niña, a cold phase El Niño Southern Oscillation (ENSO). Observations were conducted north-south transect (33.6–13.25° N) along 141.5° E meridian order to study ultraplankton assemblages various oligotrophic conditions. Analyses performed at single cell level by analytical flow cytometry. Five groups (Prochlorococcus, Synechococcus, picoeukaryotes, nanoeukaryotes...
Abstract. Studies investigating the fate of diazotrophs through microbial food web are lacking, although N2 fixation can fuel up to 50 % new production in some oligotrophic oceans. In particular, role played by heterotrophic prokaryotes this transfer is largely unknown. frame VAHINE (VAriability vertical and tropHIc diazotroph derived N south wEst Pacific) experiment, three replicate large-volume (∼ m3) mesocosms were deployed for 23 days Caledonia lagoon intentionally fertilized on day 4...
Abstract. The surface ocean receives important amounts of organic carbon from atmospheric deposition. degree bioavailability this source will determine its impact on the marine cycle. In study, potential availability dissolved (DOC) leached both desert dust and anthropogenic aerosols to heterotrophic bacteria was investigated. experimental design based 16 d incubations, in dark, a bacterial inoculum into artificial seawater amended with water-soluble Saharan (D treatment) (A aerosols, so...
Sterilization is essential for discriminating biotic responses from abiotic reactions in laboratory experiments investigating biogeochemical processes of complex natural samples. However, the conventional methods used to effectively sterilize materials or culture media do not allow sterilizing samples while maintaining balances. The aim this study was develop a low-cost and easy-to-use method obtain geochemically unmodified sterilized lacustrine coastal marine ecosystems. In preliminary...