A5043659141- Genetics, Aging, and Longevity in Model Organisms
- Genomic variations and chromosomal abnormalities
- Single-cell and spatial transcriptomics
- Cassava research and cyanide
- Cancer Genomics and Diagnostics
- Organ Transplantation Techniques and Outcomes
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Rare Diseases
- DNA Repair Mechanisms
- Bioinformatics and Genomic Networks
- Cell Image Analysis Techniques
- Genetic and phenotypic traits in livestock
- Adipose Tissue and Metabolism
- Invertebrate Immune Response Mechanisms
- Carcinogens and Genotoxicity Assessment
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- Neurobiology and Insect Physiology Research
Baylor College of Medicine
2022-2024
University of Maryland, Baltimore
2024
University of Iowa
2019-2022
New York State College of Veterinary Medicine
1995
Cornell University
1995
Aging is characterized by a decline in tissue function, but the underlying changes at cellular resolution across organism remain unclear. Here, we present Fly Cell Atlas, single-nucleus transcriptomic map of whole aging
Abstract Peripheral tissues become disrupted in Alzheimer’s Disease (AD). However, a comprehensive understanding of how the expression AD-associated toxic proteins, Aβ42 and Tau, neurons impacts periphery is lacking. Using Drosophila , prime model organism for studying aging neurodegeneration, we generated Fly Cell Atlas (AD-FCA): whole-organism single-nucleus transcriptomes 219 cell types from adult flies neuronally expressing human or Tau. In-depth analyses functional data reveal on...
Microhomology-mediated break-induced replication (MMBIR) is a DNA repair pathway initiated by polymerase template switching at microhomology, which can produce templated insertions that initiate chromosomal rearrangements leading to neurological and metabolic diseases, promote complex genomic (CGRs) found in cancer. Yet, how often accumulate from processes like MMBIR genomes poorly understood due difficulty directly identifying these events whole genome sequencing (WGS). Here, using our...
Abstract Break-induced replication (BIR) proceeds via a migrating D-loop for hundreds of kilobases and is highly mutagenic. Previous studies identified long single-stranded (ss) nascent DNA that accumulates during leading strand synthesis to be target damage primary source BIR-induced mutagenesis. Here, we describe new important mutagenic ssDNA formed BIR: the template BIR migration. Specifically, demonstrate this bottom (D-BTS) susceptible APOBEC3A (A3A)-induced lesions mutations associated...
Abstract Genome-wide association studies typically evaluate the autosomes and sometimes X Chromosome, but seldom consider Y or mitochondrial (MT) Chromosomes. We genotyped MT Chromosomes in heterogeneous stock (HS) rats (Rattus norvegicus), an outbred population created from 8 inbred strains. identified distinct 4 among founders. However, only 2 types of each nonrecombinant chromosome were observed our modern HS rat (generations 81–97). Despite relatively large sample size, there virtually...
Abstract Aging is characterized by a decline in tissue function, but the underlying changes at cellular resolution across organism remain unclear. Here, we present Fly Cell Atlas, single-nucleus transcriptomic map of whole aging Drosophila . We characterize 163 distinct cell types and perform an in-depth analysis composition, gene expression, identities. further develop clock models to predict fly age show that ribosomal expression conserved predictive factor for age. Combining all features,...
Abstract Microhomology-mediated break-induced replication (MMBIR) is a mechanism of polymerase template switching at microhomology, which can produce complex genomic rearrangements (CGRs), underlies neurological and metabolic diseases, contributes to cancer development. Yet, the extent MMBIR activity in genomes poorly understood due difficulty directly identifying events by whole genome sequencing (WGS). Here, using our newly developed MMBSearch software, we detect human report substantial...
A man who's career began as a biochemist and ended botanist. who under the instruction of Professor Butt realized what he wanted to do for rest his life. This was story Jonathan Poulton, Emeritus Faculty at University Iowa. He comes from an English family with religious background up in sciences when decided pursue biochemistry over dinner friend. earned PhD Oxford traveled abroad conduct research. Poulton eventually landed himself Iowa on frigid day but this wasn't enough deter him! became...