- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Microbial Metabolites in Food Biotechnology
- Anaerobic Digestion and Biogas Production
- Analytical Chemistry and Chromatography
- Adsorption and biosorption for pollutant removal
- Microbial Metabolism and Applications
- Genomics and Phylogenetic Studies
- Cancer Research and Treatments
- Water Quality Monitoring Technologies
- Analytical Methods in Pharmaceuticals
- Biopolymer Synthesis and Applications
- Phosphorus and nutrient management
- Biodiesel Production and Applications
- Enzyme-mediated dye degradation
- Algal biology and biofuel production
- Protein Hydrolysis and Bioactive Peptides
- Marine and fisheries research
- Computational Drug Discovery Methods
- Vibrio bacteria research studies
- Biochemical and biochemical processes
- Aquaculture disease management and microbiota
- Water Quality Monitoring and Analysis
Pandit Ravishankar Shukla University
2024
Acharya Nagarjuna University
2023
Indian Institute of Chemical Technology
2007-2022
National Institute of Ocean Technology
2008-2021
Cadila Pharmaceuticals (India)
2021
Ministry of Earth Sciences
2014-2020
Government of India
2018-2020
Aim: Modelling and optimization of fermentation factors evaluation for enhanced alkaline protease production by Bacillus circulans. Methods Results: A hybrid system feed‐forward neural network (FFNN) genetic algorithm (GA) was used to optimize the conditions enhance B. Different microbial metabolism regulating (incubation temperature, medium pH, inoculum level, volume, carbon nitrogen sources) were construct a ‘6‐13‐1’ topology FFNN identifying nonlinear relationship between enzyme yield....
Abstract Marine actinobacteria are known to be a rich source for novel metabolites with diverse biological activities. In this study, potential extracellular L-asparaginase was characterised from the Streptomyces griseus NIOT-VKMA29. Box-Behnken based optimization used determine culture medium components enhance production. pH, starch, yeast extract and L-asparagine has direct correlation enzyme production maximum yield of 56.78 IU mL −1 . A verification experiment performed validate more...
Biosorption of heavy metals by bio-materials has been posited as a potential alternative to the existing physicochemical technologies for detoxification and recovery toxic valuable from wastewaters. In this context, role mangrove leaf powder (MLP) biosorbent chromium removal was investigated. present study, effect process parameters such particle size, solution pH, initial concentration Cr(VI) ion adsorbent dose on MLP The maximum sorption observed at size 0.5 mm pH 2.0. adsorption data...
Abstract BACKGROUND: An unstructured mathematical model was developed to understand information on the relationship between Bacillus circulans growth and metabolism‐related protease production (using logistic Luedeking–Piret equations respectively) in a batch reactor with respect glucose consumption fermentation time. The objective develop an indispensable tool for optimisation, control, design analysis of alkaline production. RESULTS: Biomass enzyme titres changed change substrate...
Aim: Investigation of mixture-design impact on glutaminase production by isolated Bacillus sp. Methods and Results: An augmented simplex centroid design was used to optimize a three (wheat bran, Bengal gram husk palm seed fibre) component mixture for production. Selected substrate materials showed values at individual level [2789 U gds−1 (gram dry substrate] in two-level combination with wheat bran (maximum 3300 gds−1). Conclusion: is the most suitable medium Maximum achieved using...
Abstract BACKGROUND: Owing to the importance of glutaminase in biotech product production, its production with isolated Bacillus subtilis RSP‐GLU (MTCC 9727) was investigated. Fermentation factors play an important role enhancement. Hence, optimized using artificial neural network (ANN) coupled genetic algorithm (GA). RESULTS: A ‘6–12–1’ topology ANN constructed identify nonlinear relationship between fermentation and enzyme yield. ANN‐predicted values were for a GA. The overall mean...
Black gram husk was used as a solid substrate for laccase production by Pleurotus ostreatus, and various fermentation conditions were optimized based on an artificial intelligence method. A total of six parameters, i.e., temperature, inoculum concentration, moisture content, CuSO4, glucose, peptone concentrations, optimized. 50 experiments conducted, the obtained data modeled hybrid neural network (ANN) genetic algorithm (GA) approaches. ANN employed to model experimental data, predicted...
To identify a potential bacterium which produces antimicrobial peptide (vibriocin), and its purification, characterization production optimization. The bacteria subjected in the study were isolated from highly competitive ecological niche of mangrove ecosystem. was characterized by phenotype besides 16S rRNA gene sequence analysis. antibacterial activity recognised using agar well diffusion method. vibriocin purified ammonium sulphate precipitation, butanol extraction, gel filtration...
l-Glutaminase (l-glutamine amidohydrolase, EC 3.5.1.2) is a deamiding enzyme that has current focus on therapeutic potentials and flavor enhancement agent in food industry. It was noticed the amidase production depended bacterial growth. However, of growth by other substrates suppress secretion l-glutaminase. Governing as well challenging task. In this study multi-objective based superimposed optimization method employed to control biomass enhance Various parameters such volume medium, pH,...
For the synchronous verification of Cefadroxil and Probenecid in original form medicine dual dosages, two much sensitive accurate spectrophotometric techniques have been derived. Using these techniques, a novel idea area under curve- AUC-is put forth for calculated medicines. DDQ-(2,3-Dichloro-5,6-Dicyano-1,4- Benzoquinone) is used an expository reagent Method A, between 389nm to 691nm DDQ, applied verified. p-CA-(p-Chloranilicacid: 2,5–Dichloro-3,6– dihydroxy-1,4–benzoquinone) 399nm 701nm...