A5010291523- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Body Image and Dysmorphia Studies
- Tattoo and Body Piercing Complications
- Rabies epidemiology and control
- Neonatal Respiratory Health Research
- Vector-Borne Animal Diseases
- Allergic Rhinitis and Sensitization
- Facial Rejuvenation and Surgery Techniques
- Actinomycetales infections and treatment
- S100 Proteins and Annexins
- Infectious Diseases and Mycology
- Dermatologic Treatments and Research
- Mycobacterium research and diagnosis
- Protease and Inhibitor Mechanisms
- Yersinia bacterium, plague, ectoparasites research
- Malaria Research and Control
ID-FISH Technology (United States)
2017
University of New Haven
2012-2016
Centre for Cellular and Molecular Biology
2016
Borrelia burgdorferi, the causative agent of Lyme disease, has long been known to be capable forming aggregates and colonies. It was recently demonstrated that burgdorferi aggregate formation dramatically changes in vitro response hostile environments by this pathogen. In study, we investigated hypothesis these are indeed biofilms, structures whose resistance unfavorable conditions well documented. We studied for several hallmark features biofilm, including structural rearrangements...
Lyme borreliosis, caused by the spirochete Borrelia burgdorferi sensu lato, has grown into a major public health problem. We recently identified novel morphological form of B. burgdorferi, called biofilm, structure that is well known to be highly resistant antibiotics. However, there no evidence existence biofilm in vivo; therefore, main goal this study was determine presence infected human skin tissues. Archived biopsy tissues from borrelial lymphocytomas (BL) were reexamined for lato using...
Two rapid dual color fluorescence in situ hybridization (FISH) assays were evaluated for detecting M. tuberculosis and related pathogens cultures. The MN Genus-MTBC FISH assay uses an orange fluorescent probe specific the Mycobacterium complex (MTBC) a green Nocardia genera (MN Genus) to detect distinguish MTBC from other Mycobacteria Nocardia. A complementary MTBC-MAC probes avium (MAC) respectively identify differentiate two species complexes. are performed on acid-fast staining bacteria...
<ns4:p>Morgellons disease (MD) is an emerging multisystem illness characterized by skin lesions with unusual filaments embedded in or projecting from epithelial tissue. Filament formation results abnormal keratin and collagen expression epithelial-based keratinocytes fibroblasts. Recent research comparing MD to bovine digital dermatitis, animal infectious similar features, provided clues that spirochetal infection could play important role the human as it does illness. Based on histological...
Abstract The molecular mechanism of epimorphic regeneration is elusive due to its complexity and limitation in mammals. Epigenetic regulatory mechanisms play a crucial role development regeneration. This investigation attempted reveal the epigenetic mechanisms, such as histone H3 H4 lysine acetylation methylation during zebrafish caudal fin It was intriguing observe that H3K9,14 acetylation, H4K20 trimethylation, H3K4 trimethylation H3K9 dimethylation along with their respective genes, GCN5,...
Apicomplexan parasites of the genus Babesia cause babesiosis in humans and animals. The microscopic examination stained blood smears, detection serum antibodies by immunoassays, PCR-based identification parasite nucleic acid are common laboratory methods for diagnosing babesiosis. present study evaluated a commercially available genus-specific fluorescence situ hybridization (FISH) test detecting smears. FISH detected Babesiaduncani Babesiamicroti, two species that human infections USA,...
Plasmodium knowlesi is primarily responsible for zoonotic malaria in several Southeast Asian countries. Precise identification of the parasite blood patients presently relies on an expensive and elaborate PCR procedure because microscopic examination other available field techniques lack adequate specificity. Therefore, use a simple inexpensive dual-colour fluorescence situ hybridization (FISH) assay, analogous to FISH assays recently described falciparum vivax, was investigated as potential...
Tick-borne relapsing fever (TBRF) is caused by spirochete bacteria of the genus Borrelia termed Borreliae (RFB). TBRF shares symptoms with Lyme disease (LD) related (LDB). and LD are transmitted ticks occur in overlapping localities worldwide. Serological detection antibodies used for laboratory confirmation not established TBRF. A line immunoblot assay using recombinant proteins from different RFB species, IB, was developed its diagnostic utility investigated. The IBs were able to...
Morgellons disease (MD) is an emerging multisystem illness characterized by skin lesions with unusual filaments embedded in or projecting from epithelial tissue. Filament formation results abnormal keratin and collagen expression epithelial-based keratinocytes fibroblasts. Recent research comparing MD to bovine digital dermatitis, animal infectious similar features, provided clues that spirochetal infection could play important role the human as it does illness. Based on histological...
Apicomplexan parasites of the genus Babesia cause babesiosis in humans and animals worldwide. Human is a predominantly zoonotic disease transmitted by hard ticks that increasing health concern USA many other countries. Microscopic examination stained blood smears, detection serum antibodies immunoassays identification parasite nucleic acid qPCR fluorescence situ hybridization (FISH) are some methods available for diagnosing babesiosis. This study investigated use genus-specific FISH test...