A5088893893- Sarcoma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- Cancer-related gene regulation
- Biochemical and Molecular Research
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Signaling Pathways in Disease
- RNA Research and Splicing
- Pancreatic and Hepatic Oncology Research
- Protein Degradation and Inhibitors
- PARP inhibition in cancer therapy
- Endoplasmic Reticulum Stress and Disease
- Nuclear Structure and Function
- Chemokine receptors and signaling
- RNA regulation and disease
- Cardiac tumors and thrombi
- Immunotherapy and Immune Responses
- interferon and immune responses
- Mobile Health and mHealth Applications
- Technology Use by Older Adults
- Peroxisome Proliferator-Activated Receptors
- Lymphoma Diagnosis and Treatment
University of Michigan–Ann Arbor
2019-2022
Glioblastoma (GBM) is an aggressive primary brain cancer, with a 5 year survival of ∼5%. Challenges that hamper GBM therapeutic efficacy include (i) tumor heterogeneity, (ii) treatment resistance, (iii) immunosuppressive microenvironment (TME), and (iv) the blood-brain barrier (BBB). The C-X-C motif chemokine ligand-12/C-X-C receptor-4 (CXCL12/CXCR4) signaling pathway activated in associated progression. Although CXCR4 antagonist (AMD3100) has been proposed as attractive anti-GBM target, it...
Mitochondrial glutamate-oxaloacetate transaminase 2 (GOT2) is part of the malate-aspartate shuttle, a mechanism by which cells transfer reducing equivalents from cytosol to mitochondria. GOT2 key component mutant KRAS (KRAS*)-mediated rewiring glutamine metabolism in pancreatic ductal adenocarcinoma (PDA). Here, we demonstrate that loss disturbs redox homeostasis and halts proliferation PDA vitro. knockdown (KD) cell lines vitro induced NADH accumulation, decreased Asp α-ketoglutarate (αKG)...
Abstract The pancreatic tumor microenvironment drives deregulated nutrient availability. Accordingly, cancer cells require metabolic adaptations to survive and proliferate. Pancreatic subtypes have been characterized by transcriptional functional differences, with reported exist within the same tumor. However, it remains unclear if this diversity extends programming. Here, using metabolomic profiling interrogation of dependencies, we identify two distinct subclasses among neoplastic...
Abstract Purpose: Propagation of Ewing sarcoma requires precise regulation EWS::FLI1 transcriptional activity. Determining the mechanisms fusion will advance our understanding tumor progression. Here we investigated whether HOXD13, a developmental transcription factor that promotes metastatic phenotypes, influences Experimental Design: Existing and cell line datasets were used to define binding sites targets. Chromatin immunoprecipitation CRISPR interference employed identify enhancers....
The ageing of the population is growing significantly and will challenge healthcare systems. Chronic diseases in older require a change service delivery, new technologies can be key element ensuring viability sustainability these However, generation gap physical cognitive decline commonly associated with are barriers to transition models care. Despite this, there has been trend towards digital healthcare, which many potential benefits for population. Numerous studies have assessed...
Abstract Ewing sarcomas are driven by EWS–ETS fusions, most commonly EWS-FLI1, which promotes widespread metabolic reprogramming, including activation of serine biosynthesis. We previously reported that biosynthesis is also activated in sarcoma the scaffolding protein menin through as yet undefined mechanisms. Here, we investigated whether EWS-FLI1 and/or orchestrate via modulation ATF4, a stress-response gene acts master transcriptional regulator other tumors. Our results show sarcoma, ATF4...
ABSTRACT The tumor microenvironment (TME) in pancreatic ductal adenocarcinoma (PDA) restricts vascularization and, consequently, access to blood-derived nutrients and oxygen, which impacts growth. Intracellular redox imbalance is another restraint on cellular proliferation, yet it unknown if the TME contributes maintenance of homeostasis PDA cells. Here, we demonstrate that loss mitochondrial glutamate-oxaloacetate transaminase 2 (GOT2), a component malate-aspartate shuttle, disturbs halts...
The TeNDER project aims to improve the Quality of Life (QoL) chronic patients through an integrated care ecosystem. This study evaluates Health Recommender System (HRS) developed for project, which offers personalized recommendations based on data collected from a set monitoring devices. list notifications covered different areas daily-life such as physical activity, nutrition, and sleep. We conducted this case evaluate effectiveness usability HRS in providing accurate relevant users....
Abstract Glioblastoma multiforme (GBM) is an aggressive primary brain tumor, with poor prognosis. Major obstacles hampering effective therapeutic response in GBM are tumor heterogeneity, high infiltration of immunosuppressive myeloid cells, and the presence blood-brain barrier. The C-X-C Motif Chemokine Ligand 12/ Receptor 4 (CXCL12/ CXCR4) signaling pathway implicated invasion cell cycle progression. While CXCR4 antagonists (AMD3100) has a potential anti-GBM effects, its pharmacokinetic...
Supplementary Figure from EWS::FLI1 and HOXD13 Control Tumor Cell Plasticity in Ewing Sarcoma
<div>AbstractPurpose:<p>Propagation of Ewing sarcoma requires precise regulation EWS::FLI1 transcriptional activity. Determining the mechanisms fusion will advance our understanding tumor progression. Here we investigated whether HOXD13, a developmental transcription factor that promotes metastatic phenotypes, influences activity.</p>Experimental Design:<p>Existing and cell line datasets were used to define binding sites targets. Chromatin immunoprecipitation CRISPR...
<div>AbstractPurpose:<p>Propagation of Ewing sarcoma requires precise regulation EWS::FLI1 transcriptional activity. Determining the mechanisms fusion will advance our understanding tumor progression. Here we investigated whether HOXD13, a developmental transcription factor that promotes metastatic phenotypes, influences activity.</p>Experimental Design:<p>Existing and cell line datasets were used to define binding sites targets. Chromatin immunoprecipitation CRISPR...
Supplementary Figure from EWS::FLI1 and HOXD13 Control Tumor Cell Plasticity in Ewing Sarcoma