A5051625921- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant pathogens and resistance mechanisms
- Cellular Mechanics and Interactions
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- 14-3-3 protein interactions
- Glycosylation and Glycoproteins Research
- Plant Molecular Biology Research
- Nuclear Structure and Function
- Micro and Nano Robotics
- RNA Interference and Gene Delivery
- Protein Degradation and Inhibitors
- Modular Robots and Swarm Intelligence
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
University of Toledo
2024
University of Minnesota
2014-2023
Maharaja Sayajirao University of Baroda
2012
, the causal agent of white mold infection, is a cosmopolitan fungal pathogen that causes major yield losses in many economically important crops. Spray-induced gene silencing has recently been shown to be promising alternative method for controlling plant diseases. Based on our prior research, we focused developing spray-induced approach control by
During cell division, a mitotic spindle is built by the and acts to align stretch duplicated sister chromosomes before their ultimate segregation into daughter cells. Stretching of pericentromeric chromatin during metaphase thought generate tension-based signal that promotes proper chromosome segregation. However, it not known whether actively maintains set point tension magnitude for properly attached facilitate robust mechanochemical checkpoint signaling. By imaging tracking thermal...
Faithful chromosome segregation depends on the precise timing of chromatid separation, which is enforced by checkpoint signals generated at kinetochores. Here, we provide evidence that C-terminal domain (CTD) DNA topoisomerase IIα (Topo II) provides a novel function inner centromeres kinetochores in mitosis. We find yeast CTD required for recruitment tension kinase Ipl1/Aurora B to metaphase but not interphase. Conserved SUMOylation sites are Ipl1 recruitment. This inner-centromere distinct...
The microtubule binding protein EB1 specifically targets the growing ends of microtubules in cells, where facilitates interactions cellular proteins with plus-ends. Microtubule end targeting has been attributed to high-affinity GTP-tubulin that is present at ends. However, our 3D single-molecule diffusion simulations predicted a ~ 6000% increase arrivals open, tapered tip structures relative closed lattice conformations. Using quantitative fluorescence, single-molecule, and electron...
Significance Kinesin-14 motors represent an essential class of molecular that bind to microtubules and then walk toward the microtubule minus-end. However, whether these can interact with growing plus-ends impact lengthening remains unknown. We found could a protein resides at pull this away from end. This interaction acted disrupt growth decrease lengths in cells, likely by exerting minus-end–directed forces tip alter configuration plus-end. work demonstrates general principles for diverse...
During mitosis, sister chromatids are stretched apart at their centromeres via attachment to oppositely oriented kinetochore microtubules. This stretching generates inwardly directed tension across the separated centromeres. The cell leverages this signal detect and then correct potential errors in chromosome segregation, a mechanical signaling pathway that detaches improperly attached kinetochores from However, sequence of events leading up these detachment remains unknown. In study, we...
Accurate genome segregation in mitosis requires that all chromosomes are bioriented on the spindle. Cells monitor biorientation by sensing tension across sister centromeres. Chromosomes not have low centromere tension, which allows Aurora B (yeast Ipl1) to perform error correction locally loosens kinetochore-microtubule attachments allow detachment of microtubules and fresh attempts at achieving biorientation. However, it is known whether recruits centromeres or, alternatively, directly...
<title>Abstract</title> <italic>Sclerotinia sclerotiorum</italic>, the causal agent of white mold infection, is a cosmopolitan fungal pathogen that causes major yield losses in many economically important crops. Spray induced gene silencing has recently been shown to be promising alternative method for controlling plant diseases. Based on our prior research, we focus developing spray-induced approach control by silencing<italic>S. sclerotiorum</italic>argonaute 2 (SsAgo2), crucial part small...
Abstract The microtubule binding protein EB1 specifically targets the growing ends of microtubules in cells, where facilitates interactions cellular proteins with plus-ends. Microtubule end targeting has been attributed to high affinity GTP-tubulin that is present at ends. However, our 3D single-molecule diffusion simulations predicted a ∼6000% increase arrivals open, tapered tip structures relative closed lattice conformations. Using quantitative fluorescence, single-molecule, and electron...
<title>Abstract</title> <italic>Sclerotinia sclerotiorum</italic>, causal agent of white mold infection, is a cosmopolitan fungal pathogen that causes major yield losses in many economically important crops. Spray induced gene silencing has recently been shown to be promising alternative method for controlling plant diseases. Based on our prior research, we focus developing spray-induced approach control by silencing<italic>S. sclerotiorum</italic>argonaute 2 (SsAgo2), crucial part the small...