- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fish Ecology and Management Studies
- Fish Biology and Ecology Studies
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Gallbladder and Bile Duct Disorders
- Pediatric Hepatobiliary Diseases and Treatments
- Gastric Cancer Management and Outcomes
- Esophageal and GI Pathology
- Esophageal Cancer Research and Treatment
- Gastrointestinal disorders and treatments
- Marine and fisheries research
- Birth, Development, and Health
- Lipid metabolism and biosynthesis
- Entomological Studies and Ecology
- Pharmacological Effects and Assays
- Biliary and Gastrointestinal Fistulas
- Aquaculture disease management and microbiota
- Dysphagia Assessment and Management
- Meat and Animal Product Quality
- Hormonal and reproductive studies
- Renal and related cancers
- Genetic and Kidney Cyst Diseases
Délégation Paris 6
2006-2019
Hôpital Cochin
2011-2019
Consejo Superior de Investigaciones Científicas
1990-2017
Instituto de Acuicultura Torre de la Sal
1986-2015
Unidades Centrales Científico-Técnicas
2015
Instituto de Ciencias Marinas de Andalucía
2011-2015
Institut de Ciències del Mar
2014
Brunel University of London
1996-1999
University of Ottawa
1999
RIAs were developed for the two salmon gonadotropins (GTH I and GTH II) used to measure plasma throughout life of rainbow trout, Oncorhynchus mykiss. The RIA II was specific sensitive (< 0.001% cross-reaction with I, mean sensitivity = 0.26±0.02 ng/ml). less than (9.7% II, 2.34 ± 0.23
Background and Study Aims: Gastric outlet obstruction is a late event in the natural history of biliopancreatic tumours. Metallic self-expanding stents inserted under endoscopic fluoroscopic guidance can be used for palliation. The aim this study was to evaluate feasibility, efficacy, complications duodenal stenting patients with malignant gastric obstruction. Patients Methods: Between August 1998 November 2001, 63 (31 women, 32 men; mean age 73 ± 12) presenting clinical symptoms underwent...
Vitellogenin is the major yolk protein precursor in fish, but little known about its processing pathway oocyte, nor mobilization of proteins during embryogenesis. In this study we cloned three putative enzymes; specifically, cathepsin B and L, lipoprotein lipase (LPL), from rainbow trout ovary determined their patterns gene expression, together with D, oogenesis embryogenesis using reverse transcription-polymerase chain reaction. The approximate sizes both L transcripts were estimated as...
Partial cDNAs encoding two lipoprotein receptors were isolated and sequenced from the ovary in rainbow trout (rt), Oncorhynchus mykiss. One of cDNAs(rt-LPR) contained 5 domains characteristic belonging to low-density receptor superfamily. The second cDNA (rt- LP[OS]R) was similar rt-LPR but lacked 105 base pairs 0-linked sugar domain. deduced amino acid sequences rt-LP[OS]R had between 75% 80% identity with very-low-density vitellogenin other species. rt-lipoprotein mRNAs approximately 3.5...
We used a variety of methods to detect known gene conversions in the actin families five angiosperm species, beta-globin two primate and Zfx/Zfy seven mammalian species. Our goal was devise working strategy which would allow analysis members multigene family order determine whether there had been between its members, identify genes involved conversions, establish lengths converted regions, polarities conversions. show that three phylogenetic homoplasy test Maynard Smith perform relatively...
Rainbow trout (Oncorhynchus mykiss) were subjected to unilateral ovariectomy (ULO) during early vitellogenesis examine the endocrine responses mediating recruitment and growth of oocytes in secondary (vitellogenic) phase. ULO induced a second population primary into vitellogenic phase that then grew at faster rate than control fish. Seven days post-ULO, concentration plasma salmonid FSH (sFSH = GtH I) was significantly higher controls elevated for least 54 days. Maximal concentrations sFSH...
Under constant short photoperiod, the spawning time of 2‐year‐old sea bass Dicentrarchus labrax was advanced as compared to controls, whereas spawnings were delayed under long photoperiod. High plasma levels 17β‐oestradiol (E 2/ ) and testosterone (T) in females coincident with appearance vitellogenic oocytes ovary, while high 11‐ketotestosterone (11‐KT) T males presence spermiating males. Although E 2 11‐KT low during remainder cycle, always >1 ng ml −1 both sexes, suggesting that could...
This work examines the effects of constant long and short days on hormonal profile plasma 17β-oestradiol (E2) levels ovarian vitellogenic events, as well fecundity, time spawning egg quality sea bass. Constant first administered in April advanced (by 45 average) whereas exposure to from same date induced a delay (averaging 38 days) compared controls reared an ambient light regime (Lat. 40°N). These regimes produced shifts profiles percentage oocytes according respective groups. Relative...
The cyclic pattern of oocyte development in the sea bass, Dicentrarchus labrax L., was studied after induction spawning by two injections, 24 h apart, a luteinizing hormone releasing‐hormone analog (LHRHa) administered at end vitellogenesis. first difference developmental stage ovary and size‐frequency distribution oocytes between LHRHa treated group control group, detected 32 injection, showing higher proportion 900 μm diameter class (maturing oocytes) ( P <0.01). At 48 LHRHa‐treated...
Results are summarized concerning physiological responses of sea bass to different light and feeding regimes. Inappropriate broodstock management can cause significant alteration hormonal rhythms with important consequences for the quality eggs larvae produced.