A5047063253- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Sarcoma Diagnosis and Treatment
- Multiple Myeloma Research and Treatments
- Radiation Detection and Scintillator Technologies
- Luminescence Properties of Advanced Materials
- Metal Extraction and Bioleaching
- Extraction and Separation Processes
- Histone Deacetylase Inhibitors Research
- Studies on Chitinases and Chitosanases
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Solid-state spectroscopy and crystallography
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- Minerals Flotation and Separation Techniques
Shanghai Jiao Tong University
2024-2025
Xiamen University of Technology
2025
Tsinghua University
2020-2024
Center for Life Sciences
2020-2024
Institut de Biologie systémique et synthétique
2020
Abstract Ubiquitination, catalyzed usually by a three-enzyme cascade (E1, E2, E3), regulates various eukaryotic cellular processes. E3 ligases are the most critical components of this catalytic cascade, determining both substrate specificity and polyubiquitination linkage specificity. Here, we reveal mechanism naturally occurring E3-independent ubiquitination reaction unique human E2 enzyme UBE2E1 solving structure in complex with SETDB1-derived peptide. Guided peptide sequence-dependent...
Targeted membrane protein degradation using cell surface E3 ligases RNF43/ZNRF3 via proteolysis targeting chimeras (PROTACs) represents an effective strategy for treating drug targets that cannot be fully inhibited traditional inhibitors. Several ingenious have been developed to tether target proteins, resulting in the of at sub‐nanomolar concentrations both vitro and vivo. However, currently available binders are genetically encoded poor plasticity, which limits design promotion such...
Targeted membrane protein degradation using cell surface E3 ligases RNF43/ZNRF3 via proteolysis targeting chimeras (PROTACs) represents an effective strategy for treating drug targets that cannot be fully inhibited traditional inhibitors. Several ingenious have been developed to tether target proteins, resulting in the of at sub‐nanomolar concentrations both vitro and vivo. However, currently available binders are genetically encoded poor plasticity, which limits design promotion such...
Protein ubiquitination plays a critical role in regulating fundamental biological processes through both proteolytic and nonproteolytic mechanisms. While classically known for its targeting proteins degradation, can also modulate enzymatic activity. However, all of the mechanisms such modulation involve spatially constrained that take place near enzyme-substrate interfaces. Here, we report an unprecedented regulatory paradigm mediated by is located distal to interface but still activate...
Abstract Recent research reported an effector protein SAP05 secreted by phytoplasma could hijack hostage Rpn10 subunit of proteasome and target degradation GATA or SPL transcription factors (TFs) without ubiquitin. As a result, plant phenotypes will be reprogrammed to state suitable for amplification insect vector propagation which present prolonged growth cycles, witches’ broom-like proliferations leaves sterile shoots. We are curious about how bypassing the ubiquitin pathway if there is...
H3K9 methylation is an evolutionarily conserved hallmark of heterochromatin and plays crucial roles in chromosome segregation, genome stability, gene expression regulation. Clr4 the sole histone methyltransferase responsible for catalyzing Schizosaccharomyces pombe. K455/K472 automethylation H3K14 ubiquitination (H3K14Ub) are vital activators catalytic activity Clr4, ensuring appropriate deposition preventing deleterious silencing. While mechanism by which activates was recently elucidated,...
Abstract The cancer-specific fusion oncoprotein SS18-SSX1 disturbs chromatin accessibility by hijacking the BAF complex from promoters and enhancers to polycomb-repressed regions. This process relies on selective recognition of H2AK119Ub nucleosomes SSX1. However, mechanism which SSX1 in absence ubiquitin (Ub)-binding capacity remains unknown. Here we report cryo-EM structure bound at 3.1 Å resolution. Combined vitro biochemical cellular assays revealed that Ub is unique depends a cryptic...
Besides traditional ubiquitin-dependent proteasome degradation, thousands of eukaryotic proteins more than previously appreciated could undergo ubiquitin-independent proteasomal degradation (UbInPD). A pathogen-encoded effector protein SAP05 secreted by phytoplasma, hijack hostage Rpn10 subunit derived from Arabidopsis thaliana and target the GATA BINDING FACTOR (GATA) or SQUAMOSA-PROMOTER PROTEIN-LIKE (SPL) transcription factors without ubiquitin additional shuttle factors. To explain how...