A5105664385- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Microbial Fuel Cells and Bioremediation
- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- SARS-CoV-2 detection and testing
- Genomics and Phylogenetic Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Pluripotent Stem Cells Research
- CAR-T cell therapy research
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- COVID-19 Clinical Research Studies
Innovative Genomics Institute
2020-2023
University of California, Berkeley
2020-2023
Knowledge of microbial gene functions comes from manipulating the DNA individual species in isolation their natural communities. While this approach to genetics has been foundational, its requirement for culturable microorganisms left majority microbes and interactions genetically unexplored. Here we describe a generalizable methodology editing genomes specific organisms within complex community. First, identified tractable bacteria community using new approach, Environmental Transformation...
Abstract Mutations in the BRCA2 gene are associated with sporadic and familial cancer, cause genomic instability sensitize cancer cells to inhibition by poly(ADP-ribose) polymerase (PARP). Here we show that human pluripotent stem (hPSCs) one copy of deleted can be used annotate variants this test their sensitivities PARP inhibition. By using Cas9 edit functional allele locally haploid hPSCs fibroblasts differentiated from them, characterized essential regions identify permissive...
Abstract Cyanobacteria are central to biogeochemical cycling, climate change, and eutrophication. While they readily develop associations with environmental microorganisms, the question of whether consistently recruit specific microbiomes remains unresolved. Here, we established in vitro cyanobacterial consortia by inoculating five different strains from three freshwater environments determine if similar non-cyanobacterial organisms were recruited into stable cyanobacteria-based communities....