A5044960191- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Plant pathogens and resistance mechanisms
- Plant Pathogens and Resistance
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Phytoplasmas and Hemiptera pathogens
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Nanoparticles: synthesis and applications
- Studies on Chitinases and Chitosanases
- Fungal Biology and Applications
- Transgenic Plants and Applications
- Geochemistry and Elemental Analysis
- Insect and Arachnid Ecology and Behavior
- Heavy metals in environment
- Biochemical and Structural Characterization
- Insect and Pesticide Research
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Plant Reproductive Biology
University of Illinois Urbana-Champaign
2020-2024
Ithaca College
2013-2020
Cornell University
2014-2020
University of South Carolina
2010-2020
Summary The bacterial flagellin (FliC) epitopes flg22 and flg II ‐28 are microbe‐associated molecular patterns ( MAMP s). Although is recognized by many plant species via the pattern recognition receptor FLS 2, neither nor extent of different families known. Here, we tested significance as a importance allelic diversity in plant–pathogen interactions using purified peptides Pseudomonas syringae ∆ fliC mutant complemented with alleles. genotype were found to significantly affect immune...
The COP9 signalosome (CSN) is a multi-protein complex that regulates the activities of cullin-RING E3 ubiquitin ligases (CRLs). CRLs ubiquitinate proteins in order to target them for proteasomal degradation. CSN required proper plant development. Here we show also has profound effect on defense responses. Silencing genes subunits tomato plants resulted mild morphological phenotype and reduced expression wound-responsive response mechanical wounding, attack by Manduca sexta larvae,...
Race 1 strains of Pseudomonas syringae pv. tomato, which cause bacterial speck disease are becoming increasingly common and no simply inherited genetic resistance to such is known. We discovered that a locus in Solanum lycopersicoides, termed tomato race (Ptr1), confers P. by detecting the activity type III effector AvrRpt2. In Arabidopsis, AvrRpt2 degrades RIN4 protein, thereby activating RPS2-mediated immunity. Using site-directed mutagenesis AvrRpt2, we found that, like RPS2, activation...
Tomato (Solanum lycopersicum L.) is susceptible to many diseases including bacterial speck caused by Pseudomonas syringae pv. tomato. Bacterial disease a serious problem worldwide in tomato production areas where moist conditions and cool temperatures occur. To enhance breeding of resistant fresh-market cultivars we identified race 0 field isolate, NC-C3, P. s. North Carolina used it screen collection heirloom lines for resistance the field. We observed statistically significant variation...
Wild relatives of tomato are a valuable source natural variation in breeding, as many can be hybridized to the cultivated species (Solanum lycopersicum). Several, including Solanum lycopersicoides, have been crossed S. lycopersicum for development ordered introgression lines (ILs), facilitating breeding desirable traits. Despite utility these wild and their associated ILs, few finished genome sequences produced aid genetic genomic studies. Here we report chromosome-scale assembly...
Summary The interaction between tomato and Pseudomonas syringae pv ( Pst ) is a well‐developed model for investigating the molecular basis of plant immune system. There extensive natural variation in Solanum lycopersicum (tomato) but it has not been fully leveraged to enhance our understanding tomato– pathosystem. We screened 216 genetically diverse accessions cultivated wild species their response three strains . host was investigated using multiple strains, with available genome sequences,...
Plants mount defense responses by recognizing indicators of pathogen invasion, including microbe-associated molecular patterns (MAMPs). Flagellin, from the bacterial Pseudomonas syringae pv. tomato (Pst), contains two MAMPs, flg22 and flgII-28, that are recognized (Solanum lycopersicum) receptors Flagellin sensing2 (Fls2) Fls3, respectively, but to what degree each receptor contributes immunity whether they promote immune using same mechanisms unknown. Here, we characterized...
The plant plasma membrane is a key battleground in the war between plants and their pathogens. Plants detect presence of pathogens at using sensor proteins, many which are targeted to this lipophilic locale by way fatty acid modifications. Pathogens secrete effector proteins into cell suppress plant's defense mechanisms. These effectors able access interfere with surveillance machinery hijacking host's acylation apparatus. Despite important involvement protein both immunity pathogen...
The molecular mechanisms acting between host recognition of pathogen effectors by nucleotide-binding leucine-rich repeat receptor (NLR) proteins and mitogen-activated protein kinase (MAPK) signaling cascades are unknown. MAPKKKα (M3Kα) activates MAPK leading to programmed cell death (PCD) associated with NLR-triggered immunity. We identified a tomato M3Kα-interacting protein, SlMai1, that has 80% amino acid identity Arabidopsis brassinosteroid 1 (AtBsk1). SlMai1 domain C-terminal...
Microbial response to copper oxide nanoparticles in soils is controlled by land use rather than fate.
This study was conducted to determine whether currently prevailing isolates of Xanthomonas perforans and X. gardneri, the causal agents tomato bacterial spot in Illinois, were resistant copper. First, 133 from each pathogen tested for their multiplication on mannitol glutamate yeast agar (MGYA) amended with 0.8 mM laboratory-grade copper sulfate. Results showed that 59% 38% gardneri formed colonies copper-amended culture. Then, six five MGYA 0.2, 0.8, 1.2, 1.6, 2.0 either hydroxide or All 11...
Plant responses to the environment and developmental processes are mediated by a complex signaling network. The Arabidopsis thaliana mitogen-activated protein kinases (MAPKs) MPK3 MPK6 their orthologs in other plants shared signal transducers that respond many environmental signals thus represent highly connected hubs cellular In animals, specific MAPK complexes assembled which enable input-specific protein-protein interactions outcomes. plants, not much is known about such complexes. Here,...
Summary Wild relatives of tomato are a valuable source natural variation in breeding, as many can be hybridized to the cultivated species ( Solanum lycopersicum ). Several, including lycopersicoides , have been crossed S. for development ordered introgression lines (ILs). Despite utility these wild and their associated ILs, limited finished genomes produced aid genetic genomic studies. We generated chromosome-scale genome assembly LA2951 using PacBio sequencing, Illumina, Hi-C. identified...
Increasingly, new evidence has demonstrated variability in the epitope regions of bacterial flagellin, including harboring microbe-associated molecular patterns flg22 and flgII-28 that are recognized by pattern recognition receptors FLS2 FLS3, respectively. Additionally, because motility is known to contribute pathogen virulence chemotaxis, reductions or loss can significantly reduce fitness. In this study, we determined variations epitopes allow some but not all Xanthomonas spp. evade both...
Bacterial leaf spot disease caused by Xanthomonas cucurbitae has severely affected the pumpkin industries in Midwestern region of United States, with bacteria mainly infecting leaves and fruits, leading to significant yield losses. In this study, we utilized genomics genetics approaches elucidate X. molecular mechanisms pathogenesis during interaction its host. We generated first reference-quality whole-genome sequence type isolate compared other species, a smaller genome size fewer...
This study was conducted to evaluate the effectiveness of crop rotation in managing Xanthomonas cucurbitae, causal agent leaf spot, pumpkin fields. The using ‘Howden’ pumpkin, a susceptible cultivar, at four sites with no history cucurbit production. All were planted pumpkins first year rotations. disease developed all sites. In 3-year nonhost crops, severity spot plots significantly (P < 0.05) lower than those 2-year crops and continuous Furthermore, X. cucurbitae recovered from plant...
Abstract Bacterial spot disease of cucurbits, caused by Xanthomonas cucurbitae , is a major problem in cucurbit‐growing areas worldwide. In the Midwestern region United States, pumpkin and squash fields can have greater than 90% infected fruits, leading to high yield losses. While reference genomes are available for this bacterial species, genetic diversity between different strains populations unknown. After performing restriction‐site associated DNA sequencing (RAD‐seq) analysis X ....