A5072188298- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Yeasts and Rust Fungi Studies
- Bioenergy crop production and management
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Powdery Mildew Fungal Diseases
- Plant Disease Management Techniques
- Plant Parasitism and Resistance
- Phytoplasmas and Hemiptera pathogens
- Genetic Mapping and Diversity in Plants and Animals
- Nematode management and characterization studies
- Plant and Fungal Interactions Research
- Cocoa and Sweet Potato Agronomy
- Potato Plant Research
- Plant pathogens and resistance mechanisms
- Advances in Cucurbitaceae Research
- Cannabis and Cannabinoid Research
- Fungal Plant Pathogen Control
- Forest Management and Policy
- Cassava research and cyanide
- Genetics and Plant Breeding
Cornell University
2016-2025
Plant (United States)
2014-2025
Macclesfield District General Hospital
2021
University of Birmingham
2020
University of Amsterdam
2020
Amsterdam UMC Location University of Amsterdam
2020
Chesterfield Royal Hospital NHS Foundation Trust
2020
Doncaster and Bassetlaw Teaching Hospitals NHS Foundation Trust
2020
Dorset County Hospital NHS Foundation Trust
2020
East Kent Hospitals University NHS Foundation Trust
2020
In order to develop a diagnostic tool identify phytoplasmas and classify them according their phylogenetic group, we took advantage of the sequence diversity 16S-23S intergenic spacer regions (SRs) phytoplasmas. Ten PCR primers were developed from SR sequences shown amplify in group-specific fashion. For some groups phytoplasmas, such as elm yellows, ash pear decline, primer was paired with specific within 16S rRNA gene. Each these pairs for phytoplasma they did not produce products correct...
Dramatic changes occurred within populations of Phytophthora infestans in the United States and Canada from 1994 through 1996. Occurrence US-8 genotype, detected rarely during 1992 1993, increased rapidly predominated most regions US-7, which infected both potato tomato made up almost 50% sample was only among 330 isolates analyzed 1994. It not at all more limited samples Thus, ability to infect apparently did increase fitness this genotype relative US-8, as predicted previously. US-1,...
Abstract The cultivated potato (Solanum tuberosum) shares similar biology with other members of the Solanaceae, yet has features unique within family, such as modified stems (stolons) that develop into edible tubers. To better understand biology, we have undertaken a survey transcriptome using expressed sequence tags (ESTs) from diverse tissues. A total 61,940 ESTs were generated aerial tissues, below-ground and tissues challenged late-blight pathogen (Phytophthora infestans). Clustering...
The tomato late blight pandemic of 2009 made into a household term in much the eastern United States. Many home gardeners and many organic producers lost most if not all their crop, experiences were reported mainstream press. Some CSAs (Community Supported Agriculture) could provide tomatoes to members. In response, questions emerged: How did it happen? What was unusual about this event compared previous epidemics? is current situation 2012 what can be done? It's easiest answer these...
Abstract Hemp ( Cannabis sativa L.) is an emerging dioecious crop grown primarily for grain, fiber, and cannabinoids. There good evidence medicinal benefits of the most abundant cannabinoid in hemp, cannabidiol (CBD). For CBD production, female plants producing but not tetrahydrocannabinol (THC) are desired. We developed validated high‐throughput PACE (PCR Allele Competitive Extension) assays C. plant sex chemotype. The assay was across a wide range germplasm resolved male from monoecious...
Abstract Given the dramatic rise in high‐cannabinoid hemp ( Cannabis sativa L.) production last decade, there is an increasingly urgent need to characterize available germplasm and develop knowledge accelerate breeding of uniform stable cultivars. Despite persistent cultivation cultivars for grain or fiber around world, diversity genetic underpinning key traits are poorly understood. For 30 high‐cannabidiol replicated on two field sites, we sought evaluate yield, agronomic performance,...
Late blight of potato, caused by the oomycete pathogen Phytophthora infestans, is a devastating disease that can cause rapid death plants. To investigate molecular basis this compatible interaction, potato cDNA microarrays were utilized to identify genes differentially expressed in host during interaction with P. infestans. Of 7,680 clones represented on array, 643 (12.9%) infected plants as compared mock-inoculated control These classified into eight groups using nonhierarchical clustering...
ABSTRACT Strains of Xylella fastidiosa isolated from grape, almond, maple, and oleander were characterized by enterobacterial repetitive intergenic consensus sequence-, extragenic palindromic element (REP)-, random amplified polymorphic DNA (RAPD)-PCR; contour-clamped homogeneous electric field (CHEF) gel electrophoresis; plasmid content; sequencing the 16S-23S rRNA spacer region. Combining methods gave greater resolution strain groupings than any single method. grape with Pierce's disease...
The Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis, causal agent of bacterial wilt and canker tomato, is an economically devastating pathogen that inflicts considerable damage throughout all major tomato-producing regions. Annual outbreaks continue to occur in New York, where C. spreads via infected transplants, trellising stakes, tools, and/or soil. Globally, new can be accompanied by the introduction contaminated seed stock; however, route infection, especially role...
To design and validate a colorimetric loop‐mediated isothermal amplification assay for rapid detection of Phytophthora infestans DNA. Two sets (LAMP) primers were designed evaluated their sensitivity specificity P. infestans. ITSII targeted portion the internal transcribed spacer region ribosomal These had limit 2 pg DNA cross‐reacted with closely related species nicotianae. Rgn86_2 primers, to improve specificity, conserved hypothetical protein. 200 did not cross‐react The was tested...
Abstract Hemp ( Cannabis sativa ) is a burgeoning crop, but research‐based information about genetic and environmental effects of cannabinoid production limited will be essential for expanded cultivation. There are data available the effect stressors on content, particularly tetrahydrocannabinol (THC) in high‐cannabidiol (CBD) hemp. To address this, five stress treatments were applied replicated field trial with three high‐CBD hemp cultivars content was assayed over 3‐week time‐course...
Phytophthora infestans isolates (n = 26) collected in the Columbia Basin of Oregon and Washington 1993, which had been characterized previously for mating type, metalaxyl sensitivity, alleles at glucose-6-phosphate isomerase locus, were analyzed nuclear restriction fragment length polymorphism (RFLP) bands detected by probe RG57 mitochondrial haplotype. Analyses involving larger set markers indicated that this group satisfied expectations a sexual progeny: they contained much greater genetic...
Phytoplasmas associated with the plant diseases ash yellows (AshY, occurring in Fraxinus) and lilac witches'-broom (LWB, Syringa) represent a putative species-level taxon. Phytoplasmal DNA from 19 or sources across known geographic range of AshY (71–113 °W) was examined to determine if LWB phytoplasmas are coherent group, variability exists both conserved anonymous DNA, 16S rDNA is related host origin. The rRNA gene 16S-23S spacer were amplified using primer pair P1/P7 analysed 15...
The genomic DNA fingerprinting technique known as repetitive-sequence-based polymerase chain reaction (rep-PCR) was evaluated a tool to differentiate subspecies of Clavibacter michiganensis, with special emphasis on C. michiganensis subsp. the pathogen responsible for bacterial canker tomato. primers (REP, ERIC, and BOX), corresponding conserved repetitive element motifs in genomes diverse species, were used generate fingerprints sepedonicus, nebraskensis, tessellarius, insidiosum....
We tested the hypothesis that population of Phytophthora infestans in Toluca valley region is genetically differentiated according to habitat. Isolates were sampled three habitats from (i) wild Solanum spp. (WILD), (ii) land-race varieties low-input production systems (RURAL), and (iii) modern cultivars high-input agriculture (VALLEY). 1988-89 (n= 179) 1997-98 389). In both sampling periods, greatest genetic diversity was observed RURAL VALLEY habitats. Based on Glucose-6-phosphate isomerase...
In 2006, 2007, and 2008, we sampled 257 isolates of Phytophthora capsici from vegetables at 22 sites in four regions New York, to determine variation mefenoxam resistance population genetic structure. Isolates were assayed for genotyped mating type five microsatellite loci. We found mefenoxam-resistant a high frequency the Capital District Long Island, but none western York or central York. Both A1 A2 types 12 sites, detected 126 distinct multilocus genotypes, only nine which more than one...
Race 1 strains of Pseudomonas syringae pv. tomato, which cause bacterial speck disease are becoming increasingly common and no simply inherited genetic resistance to such is known. We discovered that a locus in Solanum lycopersicoides, termed tomato race (Ptr1), confers P. by detecting the activity type III effector AvrRpt2. In Arabidopsis, AvrRpt2 degrades RIN4 protein, thereby activating RPS2-mediated immunity. Using site-directed mutagenesis AvrRpt2, we found that, like RPS2, activation...