The functions of intrinsically disordered proteins (IDPs) are governed by relationships between information encoded in their amino acid sequences and the ensembles conformations that they sample as autonomous units. Most IDPs polyampholytes, with include both positively negatively charged residues. Accordingly, we focus here on sequence–ensemble polyampholytic IDPs. fraction residues discriminates weak strong polyampholytes. Using atomistic simulations, show polyampholytes form globules,...

Intrinsically disordered proteins and regions (IDPs) represent a large class of that are defined by conformational heterogeneity lack persistent tertiary/secondary structure. IDPs play important roles in range biological functions, their dysregulation is central to numerous diseases, including neurodegeneration cancer. The ensembles encoded amino acid sequences. Here, we present two computational tools designed enable rapid high-throughput analyses wide physicochemical properties IDP first,...

Significance Charge patterning is a key feature of intrinsically disordered protein regions. Here we test whether charge pattering important for biochemical and biological function, using the “RAM” region Notch receptor. The signaling pathway in stem-cell biology cancer. Using computer design, built 13 permutants that span broad range segregation. These have profound effects on conformational properties, binding affinity to downstream transcription factor, CSL, potency transcriptional...

Significance Intrinsically disordered regions (IDRs) of proteins are scaffolds for linear motifs that mediate protein–protein interactions and the sites posttranslational modifications. Using cell cycle inhibitory protein p27 as an archetypal example, we show patterning oppositely charged residues controls conformational properties IDRs. Charge also encodes auxiliary within We find functionalities primary modulated by a combination net charge per residue their intra-IDR result from...

Image processing is a diverging area where researches and advancements are taking geometrical progress in the agricultural field. Various going on vigorously plant disease detection. Identification of diseases can not only maximize yield production but also be supportive for varied types practices. This paper proposes detection classification technique with help machine learning mechanisms image tools. Initially, identifying capturing infected region done latter preprocessing performed....

A novel fluorescent boronate nanoprobe has been synthesized by judiciously doping boron, nitrogen, and sulphur in carbon quantum dots (BNSCQD). Specifically, the synergistic presence of nitrogen along with boronic acid provides excellent luminescence properties offers recognition sites for specific sensing glucosamine. Fluorescence intensity enhances glucosamine because agglomeration luminescent centers, thus restricts nonradiative emission channels. The detection limit this turn on...

The global dimensions and amplitudes of conformational fluctuations intrinsically disordered proteins are governed, in part, by the linear segregation versus clustering oppositely charged residues within primary sequence. Ion mobility-mass spectrometry (IM-MS) affords unique advantages for probing consequences patterning because it measures separates electrosprayed from solution on basis charge shape. Here, we use IM-MS to measure C-terminal region (p27 IDR) cell cycle inhibitory protein...

Conformational heterogeneity is a defining characteristic of proteins. Intrinsically disordered proteins (IDPs) and denatured state ensembles are extreme manifestations this heterogeneity. Inferences regarding globule versus coil formation can be drawn from analysis polymeric properties such as average size, shape, density fluctuations. Here we introduce new parameter to quantify the degree conformational within an ensemble complement descriptors. The design guided by need distinguish...

Judicious combination of fluorescence and magnetic properties along with ample drug loading capacity control release property remains a key challenge in the design nanotheranostic agents. This paper reports synthesis highly hydrophilic optically traceable mesoporous carbon nanospheres which can sustain payloads anticancer doxorubicin T2 contrast agent such as cobalt ferrite nanoparticles. The luminescent hybrid system has been prepared on silica template using resorcinol-formaldehyde...

Use of nanomaterials blessed with both therapeutic and diagnostic properties is a proficient strategy in the treatment cancer its early stage. In this context, our paper reports synthesis uniform size N-rich mesoporous carbon nanospheres 65-70 nm from pyrrole aniline precursors using Triton-X as structure-directing agent. Transmission electron microscopy reveals that these carbons spheres contain void spaces which ultrasmall nitrogen-doped quantum dots (NCQD) are captured within matrix....

Highly water dispersible, superparamagnetic carbon quantum dot (CQD) deposited Fe3O4@mTiO2 pom-pom balls have been prepared by a simple hydrothermal method. The effective deposition of dots expands the light absorption mTiO2 from UV to visible region. results photocurrent measurement reveal that an optimum C-dot at 20 wt % significantly increases density. High surface area and extended conjugation enhance adsorption organic pollutants. active species generated in photocatalytic system were...

Transport of intracellular cargos by multiple microtubule motor proteins is believed to be a common and significant phenomenon in vivo, yet signatures the microscopic dynamics systems are only now beginning resolved. Understanding these mechanisms largely depends on determining how grouping motors affect their association with microtubules stepping rates, hence, cargo run lengths velocities. We examined this problem using discrete state transition rate model collective transport. This...

Construction of a theranostic agent which integrates multiple modalities with different functions into one entity is challenging from molecular design and synthesis perspective. In this context, the present paper reports fabrication novel type multifunctional hybrid nanoparticle composed magnetic gadolinium oxide–iron oxide core, mesoporous silica shell gated boronic acid functionalized highly luminescent carbon quantum dot (BNSCQD). The porous acts as an excellent reservoir for anticancer...