A5021968324- Helicobacter pylori-related gastroenterology studies
- Galectins and Cancer Biology
- Gastric Cancer Management and Outcomes
- Veterinary medicine and infectious diseases
- Peptidase Inhibition and Analysis
- Antimicrobial Peptides and Activities
- Gastrointestinal disorders and treatments
- Gastroesophageal reflux and treatments
- Eosinophilic Esophagitis
- Amoebic Infections and Treatments
- Cancer Research and Treatments
- Biliary and Gastrointestinal Fistulas
- Parasitic Infections and Diagnostics
- Protease and Inhibitor Mechanisms
- Microbial Metabolites in Food Biotechnology
Vanderbilt University Medical Center
2022-2025
Vanderbilt University
2020-2022
Clemson University
2021
Colonization of the human stomach with cag pathogenicity island (PAI)-positive Helicobacter pylori strains is associated increased gastric cancer risk compared to colonization PAI-negative strains. To evaluate contributions Cag type IV secretion system (T4SS) and CagA (a secreted bacterial oncoprotein) molecular alterations relevant for carcinogenesis, we infected Mongolian gerbils a T4SS-positive wild-type (WT) H. strain, one two T4SS mutant (∆cagT or ∆cagY), ∆cagA 12 weeks. Histologic...
Entamoeba histolytica is a parasitic protozoan that causes amoebic dysentery, which affects approximately 90 million people each year worldwide. E. transmitted through ingestion of food and water contaminated with the cyst form, undergoes excystation in small intestine to trophozoite form colonizes large intestine. The reptile pathogen invadens has served as model for studying stage conversion between due lack reproducible encystation laboratory. Although much been learned about using...
ABSTRACT Helicobacter pylori colonization of the human stomach is a strong risk factor for gastric cancer. To investigate H. -induced molecular alterations, we used Mongolian gerbil model carcinogenesis. Histologic evaluation revealed varying levels atrophic gastritis (a premalignant condition characterized by parietal and chief cell loss) in -infected animals, transcriptional profiling loss markers these types. We then assessed spatial distribution relative abundance proteins tissues using...
The Helicobacter pylori Cag type IV secretion system (T4SS) translocates the effector protein CagA and nonprotein bacterial constituents into host cells. In this study, we infected Mongolian gerbils with an H. strain in which expression of cagUT operon (required for T4SS activity) is controlled by a TetR/tetO system. Transcript levels cagU were significantly higher gastric tissue from pylori-infected animals receiving doxycycline-containing chow (to derepress than control drug-free chow. At...
H. pylori colonization of the stomach triggers a cascade gastric alterations that can potentially culminate in cancer. The molecular occur tissue prior to development cancer are not well understood.
To evaluate potential effects of gastric inflammation on Helicobacter pylori diversification and evolution within the stomach, we experimentally infected Mongolian gerbils with an H. strain in which Cag type IV secretion system (T4SS) activity is controlled by a TetR/
Helicobacter pylori genomes encode over 60 predicted outer membrane proteins (OMPs). Several OMPs in the Hop family act as adhesins, but functions of most are unknown. To identify
Abstract Oxidative stress is a defining feature of most cancers, including those that stem from carcinogenic infections 1 . Reactive oxygen species (ROS) can drive tumor formation 2–4 , yet the molecular oxidation events contribute to tumorigenesis are largely unknown. Here we show inactivation single, redox-sensitive cysteine in host protease legumain, which oxidized during infection with gastric cancer-causing bacterium Helicobacter pylori accelerates growth. By using chemical proteomics...