Schistosomiasis constitutes a major public health problem, and 200 million people are estimated to be infected with schistosomiasis worldwide. In Brazil, has been reported in 19 states, showing areas of high medium endemicity wide range low (ALE). Barra Mansa Rio de Janeiro state an prevalence 1%. ALE represent new challenge for the helminth control because about 75% individuals asymptomatic infections occur parasite load (<100 eggs per gram feces), causing decrease sensitivity stool...

Abstract Intestinal parasitic infections are currently a source of concern for Public Health agencies in developing and developed countries. Since three ovum‐and‐parasite stool examinations have been demonstrated to provide sensitive results, we designed practical economical kit (TF‐Test) that is now commercially available (Immunoassay Com. Ind. Ltda., São Paulo, Brazil). This allows the separate collection fecal specimens into preservative solution. The then pooled, double‐filtered,...

A number of species Cryptosporidium are associated with diarrhea worldwide. Little data exists regarding the genotypes and cases infections in Brazil.In present study, we ascertained by molecular methods genotype sp from a outbreak diagnosed day care at Hospital Clínicas, São Paulo University Medical School.Specific identification typing isolates was done DNA sequencing analysis fragments amplified polymerase chain reaction (PCR) 3 different loci: SSUrRNA coding region, oocyst wall protein...

Strongyloidiasis is a potentially serious infection in immunocompromised patients. Thus, the availability of sensitive and specific diagnostic methods desirable, especially context immunosuppressed patients whom diagnosis treatment strongyloidiasis utmost importance. In this study, serological molecular tools were used to diagnose Strongyloides stercoralis infections Serum stool samples obtained from 52 Stool first analyzed by Lutz, Rugai, Agar plate culture methods, then quantitative real...

SUMMARY Strongyloides venezuelensis is a parasitic nematode of rodents that frequently used to obtain heterologous antigens for immunological diagnosis human strongyloidiasis. The aim this study was identify from filariform larvae S. immunodiagnosis Soluble and membrane fractions were obtained in phosphate saline (SS SM) Tris–HCl buffer (TS TM), analysed by Western blotting. Different antigenic components recognized IgG antibodies the sera strongyloidiasis patients. Highest recognition...

SUMMARY Blastocystis sp. is a protozoan commonly found in human and animal stool samples. Several pathogenic zoonotic aspects of this organism are still unknown. The aim the present study was to investigate subtypes (STs) samples from patients Hospital das Clínicas Faculdade de Medicina at Universidade São Paulo (HC-FMUSP), Brazil. sp.-positive diagnosed Section Parasitology Central Laboratory (HC-FMUSP) were used for DNA isolation. Polymerase chain reaction (PCR) performed using specific...

Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect low-level helminth infections common in (ALEs). This study compared Kato-Katz (KK); Hoffman, Pons, Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); qPCR for...

Blastocystis sp. is described as an enteric protist prevalent in fecal samples from humans and animals; its pathogenicity epidemiology are still controversial. Currently, it has been associated with intestinal diseases such irritable bowel syndrome clinical manifestations of allergic skin, chronic urticaria. In the context urticaria, uncertain whether this organism directly related to manifestation or just a common component microbiota. This study aimed evaluate occurrence molecular...

Parasitological diagnostic methods for schistosomiasis lack sensitivity, especially in regions of low endemicity. The objective this study was to determine the prevalence Schistosoma mansoni infections by antibody detection using indirect immunofluorescence assay (IFA-IgM) and circumoval precipitin test (COPT). Serum samples 572 individuals were randomly selected. IFA-IgM COPT used detect anti- S. antibodies. Of patients studied, 15.9% ( N = 91) positive 5.1% 29) had reactions P &lt; 0.001...

Blastocystis sp. is an intestinal protozoan commonly found in fecal samples of many animal species, including humans, but poorly studied transplant candidates. The aim this study was to evaluate the occurrence and molecular identification from A polymerase chain reaction performed using specific primers for ribosomal DNA. DNA sequences obtained were aligned compared with other GenBank MLST databases. analyzed showed a positivity 16% (24 150) highest observed renal candidates (31.4%),...

SUMMARY The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis human strongyloidiasis. Soluble and membrane S. were prepared in phosphate-buffered saline (SSF SMF, respectively), Tris-HCl (TSF TMF, an alkaline buffer (ASF AMF, respectively). Serum samples obtained patients with strongyloidiasis or, other diseases, healthy individuals analyzed by enzyme-linked immunosorbent assay (ELISA). SSF, TSF, ASF...

Blastocystis sp. is a protist commonly found in stool samples of humans and animals. Biological genetic factors this organism remain controversial. The present study aimed to develop implement the vitro culture Brazilian human isolates for routine use. fecal (n = 20) were maintained our laboratory by several passages Pavlova's medium. Cultures monitored every 72 h light microscopy. Genomic DNA was extracted identify subtypes (STs). In most isolates, vacuolar form prevalent. amoeboid,...

Amostras de sangue animais infectados com cepa Y Trypanosoma cruzi foram submetidas, respectivamente, a 200 e 300 krad radiação gama. Para verificar eficácia do método na eliminação parasita, o material foi inoculado em camundongos os parâmetros utilizados avaliação foram: parasitemia, cultura, xenodiagnóstico, subinoculação, reinoculação virulenta exame anátomo-patológico das vísceras. Os sangues expostos às duas diferentes intensidades inoculados dois períodos após processo, mostraram-se...

Abstract Strongyloidiasis can occur without any symptoms or as a potentially fatal hyperinfection disseminated infection, principally in immunosuppressed patients. Our study aimed to evaluate the application of conventional polymerase chain reaction ( cPCR ) and real‐time PCR qPCR ). Polymerase targeting 18S rRNA gene for detection Strongyloides stercoralis infection among transplant candidates were applied stool samples obtained from 150 candidates, preliminarily analyzed by parasitological...

Cyclospora cayetanensis causes watery diarrhea in tropical countries, among travelers and after ingestion of contaminated water food. Very little is known about its epidemiology, pathogenic aspects reservoirs. In Brazil, prevalence unknown to date there have been reports three outbreaks. We report here a retrospective study 5,015 stool samples from 4,869 patients attended at Clinical Hospital the University São Paulo Medical School, SP, Brazil between April 1996 January 2002, with 14 cases...

Fecal Immunochemical Test (FIT) followed by a colonoscopy is an efficacious strategy to improve the adenoma detection rate and Colorectal Cancer (CRC). There no organized national screening program for CRC in Brazil. The aim of this research was describe implementation through FIT colonoscopy, urban low-income community São Paulo city. endpoints study were: participation rate, positivity compliance Positive Predictive Values (PPV) CRC, complications. From May 2016 October 2019, asymptomatic...

Introduction: intestinal parasitic infections are common major problem closely related to poverty, inadequate sanitation, insufficient health care and overcrowding. They cause significant morbidity among institutionalized patients, however, there few studies that analyze the frequency of parasites in disabled patients not institutionalized. Objective: aim present study was determine prevalence infection their guardians. Methodology: a total 336 fecal samples were collected from 53 history...

This is the report on a patient with chronic diarrhea caused by microsporidia. He married, infected HIV and has low CD4 cell count. The diagnosis was established through stool parasite search using concentration methods Gram - chromotrope staining technique. Ileum biopsy also performed in this case. etiological may be clinical laboratory, technique routine microscopic examination of specimens.