A5089135482- Autophagy in Disease and Therapy
- Amyotrophic Lateral Sclerosis Research
- Endoplasmic Reticulum Stress and Disease
- Malaria Research and Control
- Mosquito-borne diseases and control
- Neurogenetic and Muscular Disorders Research
- Prion Diseases and Protein Misfolding
- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Invertebrate Immune Response Mechanisms
- Influenza Virus Research Studies
- Complement system in diseases
- vaccines and immunoinformatics approaches
- Parasitic Infections and Diagnostics
- Viral gastroenteritis research and epidemiology
- RNA regulation and disease
- Nerve injury and regeneration
- SARS-CoV-2 and COVID-19 Research
- Adenosine and Purinergic Signaling
- Amoebic Infections and Treatments
- Plant tissue culture and regeneration
- Genetic Syndromes and Imprinting
- Biochemical and biochemical processes
- Long-Term Effects of COVID-19
- Extracellular vesicles in disease
Universidad Mayor
2015-2024
University of Chile
2011-2018
Virginia Commonwealth University
2010-2012
Heidelberg University
2006-2010
Heidelberg University
2006
Abnormal modifications to mutant superoxide dismutase 1 (SOD1) are linked familial amyotrophic lateral sclerosis (fALS). Misfolding of wild-type SOD1 (SOD1
Although the accumulation of a misfolded and protease-resistant form prion protein (PrP) is key event in pathogenesis, cellular factors involved its folding quality control are poorly understood. PrP glycosylated disulfide-bonded synthesized at endoplasmic reticulum (ER). The ER foldase ERp57 (also known as Grp58) highly expressed brain sporadic infectious forms prion-related disorders. disulfide isomerase subset glycoproteins part calnexin/calreticulin cycle. Here, we show that levels...
ERp57 (also known as grp58 and PDIA3) is a protein disulfide isomerase that catalyzes bonds formation of glycoproteins part the calnexin calreticulin cycle. markedly upregulated in most common neurodegenerative diseases downstream endoplasmic reticulum (ER) stress response. Despite accumulating correlative evidence supporting neuroprotective role ERp57, contribution this foldase to physiology nervous system remains unknown. Here we developed transgenic mouse model overexpresses under control...
The 190-kDa merozoite surface protein 1 (MSP-1) of Plasmodium falciparum, an essential component in the parasite's life cycle, is a primary candidate for malaria vaccine. Rabbit antibodies elicited by heterologously produced MSP-1 processing products p83, p30, p38, and p42, derived from strain 3D7, were analyzed potential to inhibit vitro erythrocyte invasion parasite growth. Our data show that (i) epitopes recognized antibodies, which replication, are distributed throughout entire molecule;...
Abstract Background Plasmodium falciparum merozoites expose at their surface a large protein complex, which is composed of fragments merozoite 1 (MSP-1; called MSP-1 83 , 30 38 and 42 ) plus associated processing products MSP-6 MSP-7. During erythrocyte invasion this as well an integral membrane apical antigen-1 (AMA-1), shed from the parasite following specific proteolysis. Components MSP-1/6/7 complex AMA-1 are presently under development malaria vaccines. Methods The specificities effects...
Cryptosporidiosis is a ubiquitous infectious disease, caused by the protozoan parasites Cryptosporidium hominis and parvum, leading to acute, persistent, chronic diarrhea with life-threatening consequences in immunocompromised individuals. In developing countries, cryptosporidiosis early childhood has been associated subsequent significant impairment growth, physical fitness, intellectual abilities. Currently, vaccines are unavailable chemotherapeutics toxic impractical, agents for...
Long-term pulmonary dysfunction (L-TPD) is one of the most critical manifestations long-COVID. This lung affection has been associated with disease severity during acute phase and presence previous comorbidities, however, clinical manifestations, concomitant consequences molecular pathways supporting this condition remain unknown. The aim study was to identify characterize L-TPD in patients long-COVID elucidate main long-term attributed by analyzing parameters functional tests supported...
Creutzfeldt-Jakob disease (CJD) is the most frequent human Prion-related disorder (PrD). The detection of 14-3-3 protein in cerebrospinal fluid (CSF) used as a molecular diagnostic criterion for patients clinically compatible with CJD. However, there pressing need identification new reliable biomarkers. pathological mechanisms leading to accumulation CSF are not fully understood, however neuronal loss followed by cell lysis assumed cause increase levels, which also occurs conditions such...
Amyotrophic lateral sclerosis (ALS) is a multifactorial fatal motoneuron disease without cure. Ten percent of ALS cases can be pointed to clear genetic cause, while the remaining 90% classified as sporadic. Our study was aimed uncover new connections within network through bioinformatic approach, by which we identified C13orf18, recently named Pacer, component autophagic machinery and potentially involved in pathogenesis.Initially, Pacer using network-based analysis. Expression then...
Background Bardet-Biedl syndrome (BBS) is a ciliopathy with extensive phenotypic variability and genetic heterogeneity. We aimed to discover the gene mutated in consanguineous kindred multiple cases of BBS phenotype. Methods SNP genotype data were used for linkage analysis exome sequencing identify mutations. Modelling silico performed predict mutation severity. Results Patients had postaxial polydactyly plus variable other clinical features including rod-cone dystrophy, obesity,...
Mesenchymal stem cells (MSCs) are used in cell therapy; nonetheless, their application is limited by poor survival after transplantation a proinflammatory microenvironment. Macroautophagy/autophagy activation MSCs constitutes stress adaptation pathway, promoting cellular homeostasis. Our proteomics data indicate that RUBCNL/PACER (RUN and cysteine rich domain containing beclin 1 interacting protein like), positive regulator of autophagy, also involved death. Hence, we screened MSC upon...
Mesenchymal stem cells (MSC) have emerged as a promising tool to treat inflammatory diseases, such bowel disease (IBD), due their immunoregulatory properties. Frequently, IBD is modeled in mice by using dextran sulfate sodium (DSS)-induced colitis. Recently, the modulation of autophagy MSC has been suggested novel strategy improve MSC-based immunotherapy. Hence, we investigated possible role Pacer, enhancer, regulating immunosuppressive function context DSS-induced We found that Pacer...
Merozoites of the malaria parasite Plasmodium falciparum expose at their surface a large multiprotein complex, composed proteolytically processed, noncovalently associated products least three genes, msp-1, msp-6, and msp-7. During invasion erythrocytes, this complex is shed from except for small glycosylphosphatidylinositol-anchored portion originating MSP-1. The proteolytic cleavage separating C-terminal MSP-1 required successful invasion. Little known about structure function abundant...
Abstract Background Plasmodium falciparum infection causes cerebral malaria (CM) in a subset of patients with anti-malarial treatment protecting only about 70% to 80% patients. Why develops CM complications, including neurological sequelae or death, is still not well understood. It believed that host immune factors may modulate outcomes and there substantial evidence cellular factors, such as cytokines, play an important role this process. In study, the potential relationship between...
Herein, we report the biochemical and functional characterization of a novel Ca2+-activated nucleoside diphosphatase (apyrase), CApy, intracellular gut pathogen Cryptosporidium. The purified recombinant CApy protein displayed activity, substrate specificity calcium dependency strikingly similar to previously described human apyrase, SCAN-1 (soluble calcium-activated nucleotidase 1). was found be expressed in both Cryptosporidium parvum oocysts sporozoites, polar localization latter,...