A5055102068- Adipose Tissue and Metabolism
- Connective Tissue Growth Factor Research
- Mitochondrial Function and Pathology
- Immune Response and Inflammation
- Adipokines, Inflammation, and Metabolic Diseases
- Biomarkers in Disease Mechanisms
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Diet and metabolism studies
- Research on Leishmaniasis Studies
- Cell Adhesion Molecules Research
- Diverse Scientific Research in Ukraine
- TGF-β signaling in diseases
- Reproductive System and Pregnancy
- Fatty Acid Research and Health
- S100 Proteins and Annexins
- Angiogenesis and VEGF in Cancer
- Toxoplasma gondii Research Studies
- Mosquito-borne diseases and control
- Immune responses and vaccinations
- Global Healthcare and Medical Tourism
- Physiological and biochemical adaptations
- T-cell and B-cell Immunology
- Cancer, Hypoxia, and Metabolism
University of Geneva
2010-2019
Institut Necker Enfants Malades
2005-2008
Université Paris Cité
2007-2008
Centre National de la Recherche Scientifique
2005-2008
Inserm
2008
Délégation Paris 5
2007
The mitochondrion is a major organelle contributing to energy metabolism but also main site of ROS (reactive oxygen species) production. LPS (lipopolysaccharide)-induced signalling critical event in macrophage activation. In the present paper we report that part LPS-mediated comes from mitochondria inside signal amplification loop enhances MAPK (mitogen-activated protein kinase) More precisely, have identified inner mitochondrial membrane UCP2 (uncoupling 2) as physiological brake on...
Toxoplasma gondii possesses sets of dense granule proteins (GRAs) that either assemble at, or cross the parasitophorous vacuole membrane (PVM) and exhibit motifs resembling HT/PEXEL previously identified in a repertoire exported Plasmodium proteins. Within spp., cleavage motif by endoplasmic reticulum-resident protease Plasmepsin V precedes trafficking to export across PVM involved pathogenicity host cell remodelling. Here, we have functionally characterized T. aspartyl 5 (ASP5),...
Abstract Recruitment of circulating monocytes is critical for tumour angiogenesis. However, how human monocyte subpopulations extravasate to tumours unclear. Here we show mechanisms extravasation CD14 dim CD16 + patrolling and intermediate proangiogenic (HPMo), using xenograft models live imaging transmigration. IFNγ promotes an increase the chemokine CX3CL1 on vessel lumen, imposing continuous crawling HPMo making these insensitive chemokines required their extravasation. Expression...
Significance Upon infection, circulating leukocytes leave the bloodstream and migrate into inflammatory site. Neutrophils are first to be recruited within a few hours, followed by lymphocyte antigen 6 complex (Ly6C)-positive monocytes. This study refines model of leukocyte recruitment cascade. We demonstrate that upon Toll-like receptor 7/8-mediated vascular inflammation, platelet activation drives rapid mobilization Ly6C low monocytes luminal side endothelium. Accumulated do not extravasate...
This study focused on the stability of UCP2 (uncoupling protein 2), a mitochondrial carrier located in inner membrane mitochondrion. is very unstable, with half‐life close to 30 min, compared h for its homologue UCP1, difference that may highlight different physiological functions. Heat production by UCP1 brown adipocytes generally long and adaptive phenomenon, whereas control ROS needs more subtle regulation. We show mutation shown modify activity, actually decreases stability.
Infiltration of inflammatory cells into pancreatic islets Langerhans and selective destruction insulin-secreting β-cells are characteristics type 1 diabetes. Uncoupling protein 2 (UCP2) is a mitochondrial expressed in immune cells. UCP2 controls macrophage activation by modulating the production reactive oxygen species (ROS) MAPK signaling. We investigated role on cell activity diabetes Ucp2-deficient mice. Using model multiple low-dose streptozotocin (STZ)-induced diabetes, we found that...
Ammonia detoxification and gluconeogenesis are major hepatic functions mutually connected through amino acid metabolism. The liver is rich in glutamate dehydrogenase (GDH) that catalyzes the reversible oxidative deamination of to α-ketoglutarate ammonia, thus bridging acid-to-glucose pathways. Here we generated inducible liver-specific GDH-knockout mice (HepGlud1-/- ) explore role GDH on metabolic homeostasis. Investigation nitrogen metabolism revealed altered ammonia homeostasis HepGlud1-/-...
Leukocyte recruitment is an important process in combating pathogens. The largest class of circulating leukocytes are neutrophils, which rapidly invade inflamed tissue, followed by inflammatory Ly6C+ monocytes. Ly6Clow monocytes patrol the endothelial wall routinely steady state. We recently reported early luminal monocytes, preceded and orchestrated neutrophil arrival extravasation response to TLR7/8-mediated vascular inflammation. Here we dissected kinetics neutrophils examined dynamics...
Hemostasis is a tightly regulated physiological process to rapidly induce hemostatic plugs at sites of vascular injury. Inappropriate activation this may lead thrombosis, i.e. pathological blood clot formation in uninjured vessels or on atherosclerotic lesions. ATP release through Pannexin1 (Panx1) membrane channels contributes collagen-induced platelet aggregation vitro.To investigate the effects genetic and pharmacological inhibition Panx1 hemostasis thrombosis vivo.Bleeding time after...
The recruitment of dendritic cells to sites infections and their migration lymph nodes is fundamental for antigen processing presentation T cells. In the present study, we showed that antibody blockade junctional adhesion molecule C (JAM-C) on endothelial removed JAM-C away from junctions increased vascular permeability after L. major infection. This has multiple consequences output immune response. resistant C57BL/6 susceptible BALB/c mice, found higher numbers innate migrating blood site...
Efficient immune response is dependent on rapid mobilization of blood leukocytes to the site infection or injury. Investigating leukocyte migration in vivo crucial for understanding molecular basis transendothelial and interaction with vascular endothelium. One powerful approach involves intravital microscopy transgenic mice expressing fluorescent proteins cells interest. Here we present a protocol imaging monocytes neutrophils CX3CR1gfp/wt mouse i.v. injected orange dye-labeled an inverted...
Efficient immune response is dependent on rapid mobilization of blood leukocytes to the site infection or injury. Investigating leukocyte migration in vivo crucial for understanding molecular basis transendothelial and interaction with vascular endothelium. One powerful approach involves intravital microscopy transgenic mice expressing fluorescent proteins cells interest. Here we present a protocol imaging monocytes neutrophils CX3CR1gfp/wt mouse i.v. injected orange dye-labeled an inverted...
Abstract T-cell development is dependent on microenvironments generated by stromal cells such as thymic epithelial (TEC). TEC population, which phenotypically and functionally heterogeneous, controls the migration, proliferation selection of differentiating thymocytes. CYR61 (cysteine-rich protein 61) a matricellular reported to promote migration cancer cells. Here we investigated impact treatment development. We found that was strongly expressed in favored TEC/thymocyte interaction binding...
Abstract Dendritic cells (DCs) are recruited to sites of infection where they process antigens and migrate the draining lymph node in order trigger specific T cell responses. In C57BL/6J mouse model cutaneous Leishmaniasis, monocyte-derived dendritic were shown initiate protective Th1 response against parasite. Junctional adhesion molecule C (JAM-C), a tight junction protein expressed on endothelial fibroblasts, was described past few years control leukocyte migration during inflammation....