A5100632117- Cancer-related molecular mechanisms research
- Hippo pathway signaling and YAP/TAZ
- Dermatologic Treatments and Research
- MicroRNA in disease regulation
- Pregnancy and preeclampsia studies
- RNA modifications and cancer
- Cancer Cells and Metastasis
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Circular RNAs in diseases
- RNA Research and Splicing
- Wound Healing and Treatments
- Epigenetics and DNA Methylation
- Antibiotic Resistance in Bacteria
- Single-cell and spatial transcriptomics
- Developmental Biology and Gene Regulation
- Birth, Development, and Health
- Ubiquitin and proteasome pathways
- Fibroblast Growth Factor Research
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Congenital heart defects research
- Sinusitis and nasal conditions
- Asthma and respiratory diseases
- Pluripotent Stem Cells Research
Capital Medical University
2016-2025
Beijing Tongren Hospital
2016-2025
Affiliated Hospital of Hebei University
2025
Hunan Agricultural University
2025
Nanjing Medical University
2015-2024
Nanjing Maternity and Child Health Care Hospital
2015-2024
Zhejiang University
2024
Sir Run Run Shaw Hospital
2024
Jiangnan University
2024
Chinese Academy of Sciences
2015-2024
The heart is the central organ of circulatory system, and its proper development vital for maintaining human life. Here, we used single-cell RNA sequencing to profile gene expression landscapes ∼4,000 cardiac cells from embryos identified four major types cells: cardiomyocytes (CMs), fibroblasts, endothelial (ECs), valvar interstitial (VICs). Atrial ventricular CMs acquired distinct features early in development. Furthermore, both fibroblasts show stepwise changes expression. As proceeds,...
Single-cell epigenome sequencing techniques have recently been developed. However, the combination of different layers in an individual cell has not yet achieved. Here, we developed a single-cell multi-omics technology (single-cell COOL-seq) that can analyze chromatin state/nucleosome positioning, DNA methylation, copy number variation and ploidy simultaneously from same mammalian cell. We used this method to reprogramming state methylation mouse preimplantation embryos. found within < 12 h...
Abstract Background Colorectal cancer (CRC) ranks as the second-leading cause of cancer-related death worldwide with metastases being main death. Here, we investigated genomic and transcriptomic alterations in matching adjacent normal tissues, primary tumors, metastatic tumors CRC patients. Methods We performed whole genome sequencing (WGS), multi-region exome (WES), simultaneous single-cell RNA-Seq, targeted cDNA Sanger on from 12 colorectal patients ( n =84 for genomes, =81 exomes, =9120...
Abstract Background An epidemiological association among asthma, blood eosinophil level and chronic rhinosinusitis (CRS) is well established, but whether consistent genetic relationships exist, this reflects a shared etiology between CRS asthma or remains unclear. Methods Data from patients ( N = 1,255) healthy controls 1,032) were reviewed retrospectively to investigate associations clinical characteristics CRS. white cells in the UK biobank 173,480), Trans-National Asthma Genetic...
We report three new water-soluble dihydrofluorescein-ether probes FCN1, FCN2 and FCN3 for the detection of hypochlorous acid (HOCl), which were designed on basis a specific HOCl-promoted oxidation reaction. This work also provided useful method to monitor accumulated HOCl in organelles using zebrafish model.
Abstract Background Patient-derived organoid culture is a powerful system for studying the molecular mechanisms of cancers, especially colorectal cancer (CRC), one most prevalent cancers worldwide. There are two main types 3D methods colonic cells, but similarities and differences between gene expression patterns in different media remain largely unexplored. Results Here, we establish patient-derived organoids from patients perform single-cell RNA-Seq pairwise samples seven both their...
<h3>Background</h3> Paroxysmal dyskinesias (PDs), a clinically and genetically heterogeneous group of episodic movement disorders, include kinesigenic PD (PKD), exercise-induced (PED) non-kinesigenic (PNKD). These disorders are all transmitted as autosomal dominant traits with incomplete penetrance. Several PD-related genetic including PKD familial infantile convulsions paroxysmal choreoathetosis (ICCA), mapped to the same region on chromosome 16. Independent studies have recently revealed...
<h3>Background</h3> Paroxysmal kinesigenic choreoathetosis (PKC) is characterised by recurrent and brief attacks of involuntary movement, inherited as an autosomal dominant trait with incomplete penetrance. A PKC locus has been previously mapped to the pericentromeric region chromosome 16 (16p11.2-q12.1), but causative gene remains unidentified. <h3>Methods/results</h3> Deep sequencing this 30 Mb enriched array capture in five affected individuals from four Chinese families detected two...
Yellow catfish (Pelteobagrus fulvidraco) is one of the most important freshwater aquaculture species in China. However, its small size and lower meat yield limit edible value. Myostatin (MSTN) a negative regulator mammalian muscle growth. But, function Mstn fish remains elusive. To explore roles mstn gene growth create strain yellow with high amount mass, we performed targeted disruption using engineered zinc-finger nucleases (ZFNs). Employing zebrafish embryos as screening system to...
Intrauterine growth retardation (IUGR) induces metabolic syndrome, which is often characterized by insulin resistance (IR), in adults. Previous research has shown that microRNAs (miRNAs or miRs) play a role the target genes involved this process, but mechanisms remain unclear. In present study, we examined miRNA profiles using samples of skeletal muscles from both IUGR and control rat offspring whose mothers were fed either protein-restricted diet normal amounts protein during pregnancy,...
Visual Display Terminal Use Increases the Prevalence and Risk of Work‐related Musculoskeletal Disorders among Chinese Office Workers: A Cross‐sectional Study: Shanshan WU, et al . Department Occupational Environmental Health, School Public Peking University, P.R. China— Objectives Little is known about musculoskeletal disorders (WMSDs) computer users mainland China. The aim this study was to estimate prevalence WMSDs in office workers Beijing, determine risk factors for neck (WMSDs‐N)....
Background/Aims: Exosomes are extracellular vesicles that involved in several biological processes. The roles of proteins from human umbilical cord blood exosomes the pathogenesis preeclampsia remains poorly understood. Methods: In this study, we used high-resolution LC-MS/MS technologies to construct a comparative proteomic profiling between normal and preeclamptic pregnancies. Results: A total 221 were detected exosomes, with 14 upregulated 15 downregulated definitively identified control...
Naive hypomethylated embryonic pluripotent stem cells (ESCs) are developmentally closest to the preimplantation epiblast of blastocysts, with potential contribute all tissues and germline, excepting extra-embryonic in chimeric embryos. By contrast, (EpiSCs) resembling postimplantation relatively more methylated show a limited for chimerism. Here, first time, we reveal advanced (ASCs), which beyond inner cell mass but higher potency than EpiSCs. Accordingly, single ASC contributes very...
Objective Familial adenomatous polyposis (FAP) is characterised by the development of hundreds to thousands adenomas at different evolutionary stages in colon and rectum that will inevitably progress adenocarcinomas if left untreated. Here, we investigated genetic alterations transcriptomic transitions from precancerous adenoma carcinoma. Design Whole-exome sequencing, whole-genome sequencing single-cell RNA were performed on matched adjacent normal tissues, multiregionally sampled...
CRISPR/Cas-based systems are highly attractive for developing next-generation diagnostic technologies because of their intrinsic merits such as simplicity, sensitivity, and specificity. However, currently, nucleic acid amplification procedures still needed to achieve attomolar sensitivity in most assays, which causes high cost, operation difficulty, low efficiency. Herein, we combine the CRISPR/Cas12a-based assay a single-microbead detection platform one-step amplification-free DNA at...
The objective of this study was to investigate the distribution extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolates in swine and poultry farms China. Rectal or cloaca swabs chicken were collected from four province-level regions China, E. recovered tested for antimicrobial susceptibility. producing ESBLs further characterized by pulsed-field gel electrophoresis (PFGE) sequence analysis genes encoding β -lactamases class I integrons. In total, 156 224 rectal six farms,...
Long non-coding RNAs (lncRNAs) are thought to play crucial roles in human diseases. However, the function of lncRNAs hypertrophic scar formation remains poorly understood.In this study, we investigated expression lncRNA8975-1 tissues and fibroblasts by quantitative reverse transcription PCR (qRT-PCR). To investigate its function, overexpression knockdown were performed using lentivirus infection Stealth RNAi transfection, respectively. Cell proliferation was detected CCK-8 assay. The protein...