A5045574298- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant responses to water stress
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Plant Virus Research Studies
- RNA Research and Splicing
- Transgenic Plants and Applications
- Light effects on plants
- Plant nutrient uptake and metabolism
Pusan National University
2014-2025
The EMBRYONIC FLOWER (EMF) 1 gene has been shown to be necessary for maintenance of vegetative development. To investigate the molecular mechanism EMF1-mediated plant development, we screened EMF1-interacting proteins and identified 11 candidate using yeast two-hybrid system. Among genes, three EMF1-Interacting Protein (EIP) EIP1, EIP6 EIP9, are predicted encode a WNK (with-no-lysine) kinase, B-box zinc-finger protein DnaJ-domain protein, respectively. expression patterns EIP9 were similar...
In the signal transduction network, from perception of stress signals to stress-responsive gene expression, various transcription factors and cis-regulatory elements in promoters coordinate plant adaptation abiotic stresses. Among AP2/ERF factor family, group VII ERF (ERF-VII) genes, such as RAP2.12, RAP2.2, RAP2.3, AtERF73/HRE1, AtERF71/HRE2, are known be involved response hypoxia Arabidopsis. Notably, HRE2 has been reported responses osmotic stress. this study, we dissected promoter...
Despite increasing reports that CCCH zinc finger proteins function in plant development and stress responses, the functions molecular aspects of many remain uncharacterized. Here, we characterized biological AtC3H17 , a unique Arabidopsis gene encoding non-tandem protein. was ubiquitously expressed throughout life cycle plants their organs. The rate ratio seed germination atc3h17 mutants were slightly slower lower, respectively, than those wild type (WT), whereas -overexpressing transgenic...
Plants adapt to abiotic stresses by complex mechanisms involving various stress-responsive genes. Here, we identified a DEAD-box RNA helicase (RH) gene, AtRH17, in Arabidopsis, involved salt-stress responses using activation tagging, useful technique for isolating novel AT895, an tagging line, was more tolerant than wild type (WT) under NaCl treatment during germination and seedling development, AtRH17 activated AT895. possesses nine well-conserved motifs of RHs, consisting Q, I, Ia, Ib,...
Plant roots show distinct gene-expression profiles from those of shoots under abiotic stress conditions. In this study, we performed mRNA sequencing (mRNA-Seq) to analyze the transcriptional profiling Arabidopsis osmotic conditions—high salinity (NaCl) and drought (mannitol). The demonstrated significantly changes aerial parts both NaCl mannitol treatment. We identified 68 closely connected transcription-factor genes involved in stress-signal transduction roots. Well-known abscisic acid...
Previously, we reported that overexpression of AtRH17, an Arabidopsis DEAD-box RNA helicase gene, confers salt stress-tolerance via a pathway other than the well-known stress-responsive pathways. To decipher in AtRH17-overexpressing transgenic plants (OXs), performed RNA-Sequencing and identified 397 differentially expressed genes between wild type (WT) AtRH17 OXs. Among them, 286 were upregulated 111 downregulated OXs relative to WT. Gene ontology annotation enrichment KEGG analysis showed...
Salinity causes widespread crop loss and prompts plants to adapt through changes in gene expression. In this study, we aimed investigate the function of non-tandem CCCH zinc-finger (non-TZF) protein AtC3H3 response salt stress Arabidopsis. AtC3H3, a from non-TZF family known for its RNA-binding RNase activities, was up-regulated under osmotic stress, such as high drought. When overexpressed Arabidopsis, improved tolerance but not drought stress. The expression well-known abscisic acid...
Despite increasing reports on the function of CCCH zinc finger proteins in plant development and stress response, functions molecular aspects many non-tandem (non-TZF) remain uncharacterized. AtC3H59/ZFWD3 is an Arabidopsis non-TZF protein belongs to ZFWD subfamily harboring a motif WD40 domain. In this study, we characterized biological AtC3H59, which subcellularly localized nucleus. The seeds AtC3H59-overexpressing transgenic plants (OXs) germinated faster than those wild type (WT),...
CCCH zinc finger proteins are a large protein family and classified as either tandem (TZF) or non-TZF proteins. The roles of TZF genes in several plants have been well determined, whereas the functions many remain uncharacterized. Herein, we describe biological molecular AtC3H12, an Arabidopsis containing three motifs. AtC3H12 has orthologs plant species but no paralog Arabidopsis. AtC3H12-overexpressing transgenic (OXs) germinated slower than wild-type (WT) plants, atc3h12 mutants faster WT...
AtERF73/HRE1 is an AP2/ERF transcription factor in Arabidopsis and has two distinct alternative splicing variants, HRE1α HRE1β. In this study, we examined the differences between molecular functions of We found that HRE1β are both involved hypoxia response root development have transactivation activity. Two conserved motifs C-terminal region HRE1β, EELL LWSY-like, contributed to their activity, specifically four E residues motif MGLWS amino acid sequence at end LWSY-like motif. The...
애기장대에서 AtERF71/HRE2는 핵에서 전사인자로 작용하여 하위 유전자의 발현을 증가시키는 역할을 수행함으로써 저산소와 삼투 스트레스 반응에 관여할 것으로 여겨지는 유전자이다. 본 연구에서는 AtERF71/HRE2에 의해 직, 간접적으로 발현이 조절되는 유전자를 알아보기 위해 AtERF71/HRE2 과발현체를 대상으로 마이크로어레이 실험을 수행하였다. 야생형에 비해 과발현체에서 2배 이상 증가한 기능이 알려진 유전자는 자신을 제외하고 161개였다. 161개 유전자 중 전사인자와 DNA-결합 단백질 등과 같은 전사조절자가 24개로 확인되어, 전사조절 발현 조절을 통해 더 많은 조절하는 상위 전사인자로서의 기능을 가질 추정되었다. 15개 RT-PCR을 수행하여 결과의 신뢰성을 검증하였다. Genevestigator 데이터베이스 분석 결과, 51개 저산소 및 스트레스에 증가하는 확인되었다. RT-PCR 결과 3개 유전자가 저산소에 증가하였고, 다른 증가하였으며, 이러한 결과는...
유전자를 이소성으로 발현하고 억제하는 것은 유전자의 기능 연구에 있어서 매우 유용하다. 본 연구에서는 표적 동시 과발현, 조직/발달 단계 특이적 발현 및 스트레스 유도성 발현을 위해 pPZP를 골격으로 다양한 binary 벡터를 제작하고 그 유용성을 검증하였다. 변형된 CaMV 35S 프로모터를 이용하여, 다른 두 개의 과발현시키는 제작하였고, 이 벡터가 동시에 그리고 같은 장소에서 과발현 하는데 효과적임을 확인하였다. At2S3, KNAT1 LFY 포함하는 조직 또는 발달 벡터들을 분석한 결과, 벡터들은 각각 배/유식물 시기, 새싹 끝의 분열조직 잎 원기 발현에 유용하였다. RD29A와 AtNCED3 고염, ABA, MV 저온과 비생물성 스트레스에 의한 이소성 연구에서 제작된 통해 생물학적 기능연구, 분자생물학적작용 기작 유용하게 사용될 것으로 사료된다. In this study, we constructed various kinds of vectors with the...
식물에서 유전자의 기능을 연구하는데 있어서 유전자가 과발현 되거나 발현이 억제되는 형질전환체는 해당 기능과 관련되어 매우 유용한 정보를 제공한다. 본 연구에서는 modified CaMV 355, UBQ3, UBQ10 프로모터를 pPZP211 벡터에 각각 클로닝 하여 Agrobacterium을 매개로 한 과발현형질전환 식물체 제작에 유용하게 이용할 수 있는 pFGL571, pFGL846, pFGL847을 제조하였다. 이 벡터들은 크기가 작고, 박테리아 내에 high copy로 존재하며, 다중 부위에 다양한 제한효소 부위를 가지고 있고, 전체 서열이 알려져 등의 장점을 있다. GUS 또는 sGFP 리포터 유전자를 포함하는 형질전환 식물체를 제조하여 35S, 프로모터의 활성을 분석한 결과, 세 프로모터 모두 발아 후 대부분의 발달단계와 성숙한 식물체의 꽃 기관에서 높은 보였다. 한편, 유전자 발현 억제에 RNAi 기본 벡터인 pFGL727을 제조하였고, 이용한 벼 형질전환체의...