A5015907949- Sirtuins and Resveratrol in Medicine
- Malaria Research and Control
- Tuberculosis Research and Epidemiology
- Biochemical and Molecular Research
- Mycobacterium research and diagnosis
- Cancer-related Molecular Pathways
- Biochemical effects in animals
- Adipose Tissue and Metabolism
- RNA regulation and disease
- Autophagy in Disease and Therapy
- HIV/AIDS drug development and treatment
- RNA modifications and cancer
- Hepatitis B Virus Studies
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- 14-3-3 protein interactions
- SARS-CoV-2 and COVID-19 Research
- Aquaculture disease management and microbiota
- Cancer-related gene regulation
- Adenosine and Purinergic Signaling
- Toxoplasma gondii Research Studies
- Genomic variations and chromosomal abnormalities
- Virology and Viral Diseases
- Pneumonia and Respiratory Infections
- Chromatin Remodeling and Cancer
Center for Global Health
2020-2024
Cornell University
2020-2024
Weill Cornell Medicine
2020-2024
University of Maryland, Baltimore
2017-2019
Massachusetts Institute of Technology
2008-2019
Indian Institute of Science Bangalore
2019
Sri Devaraj Urs Medical College
2019
Sri Devaraj Urs Academy of Higher Education and Research
2019
National Institute of Allergy and Infectious Diseases
2016
VA West Los Angeles Medical Center
2002-2014
Research Article30 January 2013Open Access SIRT1 regulates differentiation of mesenchymal stem cells by deacetylating β-catenin Petra Simic Glenn Laboratory for the Science Aging, Department Biology, Massachusetts Institute Technology, Cambridge, MA, USA Search more papers this author Kayvan Zainabadi Eric Bell David B. Sykes Center Regenerative Medicine, General Hospital, Boston, Borja Saez Sutada Lotinun Oral Harvard School Dental Infection, and Immunity, Roland Baron Scadden Ernestina...
Overexpression or pharmacological activation of SIRT1 has been shown to extend the lifespan mice and protect against aging-related diseases. Here we show that protects in two models osteoporosis. Ovariectomized female aged male mice, for post-menopausal osteoporosis, respectively, significant improvements bone mass upon treatment with agonist, SRT1720. Further, find calorie restriction (CR) results a two-fold upregulation sirt1 mRNA expression tissue is associated increased CR mice....
Acquired antimalarial drug resistance produces treatment failures and has led to periods of global disease resurgence. In Plasmodium falciparum, is known arise through genome-level changes such as mutations gene duplications. We now report an epigenetic mechanism involving genes responsible for the plasmodial surface anion channel, a nutrient channel that also transports ions compounds at host erythrocyte membrane. Two blasticidin S-resistant lines exhibited markedly reduced expression clag...
Greater Mekong Subregion countries are committed to eliminating Plasmodium falciparum malaria by 2025. Current elimination interventions target infections at parasite densities that can be detected standard microscopy or rapid diagnostic tests (RDTs). More sensitive detection methods have been developed detect lower density "asymptomatic" may represent an important transmission reservoir. These ultrasensitive polymerase chain reaction (usPCR) used identify populations for mass drug...
Activation of SIRT1 has previously been shown to protect mice against osteoporosis through yet ill-defined mechanisms. In this study, we outline a role for as positive regulator the master osteoblast transcription factor, RUNX2. We find that ex vivo deletion sirt1 leads decreased expression runx2 downstream targets, but not itself, along with reduced differentiation. Reciprocally, treatment agonist promotes differentiation, well in SIRT1-dependent manner. Biochemical and luciferase reporter...
Standard methods for enumerating Mycobacterium tuberculosis in patient sputum can miss large populations of viable M. cells that are unable to grow either on solid medium or liquid unless the has been extensively diluted. Because these bacteria be detected after limiting dilution, they have termed differentially culturable detectable (DD-Mtb). Treatment with isoniazid (H), rifampin (R), pyrazinamide (Z), and ethambutol (E) (HRZE) 1 2 weeks shown increase representation DD-Mtb drug-sensitive...
Abstract After an initial wave of coronavirus disease 2019 (COVID-19) in Haiti summer 2020 (primarily lineage B.1), seropositivity for anti-severe acute respiratory syndrome 2 (SARS-CoV-2) immunoglobulin G (IgG) was ~40%. Variant P.1 (gamma) introduced February 2021, with initially limited introduction followed by exponential local dissemination within this unvaccinated population prior exposure to earlier SARS-CoV-2 lineages.
We examined the correlation between three different methods of
Mutational analysis of the Plasmodium falciparum kelch 13 (k13) gene is routinely performed to track emergence and spread artemisinin resistance. Surveillance resistance markers has been impeded by difficulty extracting sufficient DNA from low parasite density infections common in low-transmission settings, such as Southeast Asia. This problem can be overcome collecting large volumes venous blood. Efficient methods for amplifying k13 dried blood spots (DBS) would facilitate surveillance....
Abstract Diagnostics that more accurately detect and quantify viable Mycobacterium tuberculosis (Mtb) in the sputum of patients undergoing therapy are needed. Current culture- molecular-based tests have shown limited efficacy for monitoring treatment response TB patients, either due to presence sub-populations Mtb which fail grow under standard culture conditions (termed differentially detectable/culturable Mtb, DD Mtb) or prolonged half-life DNA sputum. Here, we report an optimized...
Urine holds great promise as a non-invasive sampling method for molecular diagnostics. The cell-free nucleic acids of urine however are small, labile, and difficult to purify. Here an efficient the purification these is presented. An empirically derived protocol was devised by first identifying conditions that allowed recovery 100 base pair (bp) DNA, followed optimization using quantitative polymerase chain reaction (qPCR) assay. resulting efficiently purifies both small sized DNAs RNAs from...
SIRT1 is an NAD+-dependent deacetylase that acts as a nutrient sensitive regulator of longevity. also key mesenchymal stem cells (MSCs), adult give rise to tissues such bone, fat, muscle and cartilage. This review focuses on how regulates the self-renewal, multipotency differentiation MSCs. The variable role in promoting MSCs towards certain lineages, while repressing others, will be examined within broader context aging, calorie restriction, regenerative medicine. Finally, recent animal...
Certain populations of Mycobacterium tuberculosis go undetected by standard diagnostics but can be enumerated using limiting dilution assays. These differentially detectable M. (DD tuberculosis) may have relevance for persistence due to their drug tolerance. It is unclear how well DD from patients modeled a recently developed
Abstract Studies on nontumorigenic and tumorigenic human cell hybrids derived from the fusion of HeLa (a cervical cancer line) with GM00077 normal skin fibroblast have demonstrated “functional” tumor‐suppressor activity chromosome 11. It has been shown that several neoplastically transformed radiation‐induced hybrid cells called GIMs (gamma ray induced mutants), isolated CGL1 cells, lost one copy We hypothesized, therefore, remaining gene might be mutated in cytogenetically intact Because a...
The role of sirtuin-1 (SIRT1) in innate immunity, and particular the influence SIRT1 on antimicrobial defense against infection, has yet to be reported but is important define since inhibitors are being investigated as therapeutic agents treatment cancer, Huntington's disease, autoimmune diseases. Given potential suppression, we sought characterize host defense. Utilizing both pharmacologic methods a genetic knockout, demonstrate that expression little macrophage neutrophil functions....
Cloning and sequencing of 5.5 kb deletion at chromosome 11q13.1 from the HeLa cells, tumorigenic hybrids two fibroblast cell lines have revealed homologous recombination between AluSx AluY resulting in intervening sequences. Long-range PCR sequence 494 normal lymphocyte samples showed heterozygous 28.3% African-American ancestry but only 4.8% Caucasian (p < 0.0001). This observation is strengthened by copy number variation (CNV) data HapMap which that this occurs 27% YRI (Yoruba — West...
Malaria infected erythrocytes show increased permeability to a number of solutes important for parasite growth as mediated by the Plasmodial Surface Anion Channel (PSAC). The P. falciparum clag3 genes have recently been identified key determinants PSAC, though exactly how they contribute channel function and whether additional host/parasite proteins are required remain unknown. To begin answer these questions, I taken biochemical approach. Here used an epitope-tagged CLAG3 perform...