Abstract Background Exosomes derived from mesenchymal stem cells (MSCs) have shown to effective application prospects in the medical field, but exosome yield is very low. The production of mimetic vesicles (EMVs) by continuous cell extrusion leads more EMVs than exosomes, whether protein compositions MSC-derived (MSC-EMVs) and exosomes (MSC-exosomes) are substantially different remains unknown. purpose this study was conduct a comprehensive proteomic analysis MSC-EMVs MSC-exosomes simply...

Osteosarcoma is a bone tumor with high incidence in children and adolescents. Chemotherapy for osteosarcoma limited, effective targeted drugs are urgently needed to treat osteosarcoma. Exosomes as natural nano drug delivery platform have been widely studied proven good performance. However, the low production of exosomes hinders its development carrier. Exosome mimetics (EMs) an alternative product solve problem maintain performance carriers. In this study, marrow mesenchymal stem cells...

Background Osteosarcoma (OSC) and Ewing's sarcoma (EWS) are children's most common primary bone tumors. The purpose of the study is to develop validate a new nomogram predict cancer-specific survival (CSS) childhood OSC EWS. Methods clinicopathological information all children with EWS from 2004 2018 was downloaded Surveillance, Epidemiology, End Results (SEER) database. Univariate multivariate Cox regression analyses were used screen independent risk factors for CSS. These construct CSS A...

Abstract Objective Thyroid carcinoma (TC) is the most common endocrine tumor in human body. Papillary thyroid (PTC) accounts for more than 80% of cancers. Accurate prediction elderly PTC can help reduce mortality patients. We aimed to construct a nomogram predicting cancer-specific survival (CSS) patients with PTC. Methods Patient information was downloaded from Surveillance, Epidemiology, and End Results (SEER) program. Univariate multivariate Cox regression models were used screen...

Purpose: Develop and validate an accessible prediction model using machine learning (ML) to predict the risk of chemotherapy-induced myelosuppression (CIM) in children with Wilms' tumor (WT) before chemotherapy is administered, enabling early preventive management. Methods: A total 1433 cycles 437 WT who received our hospital from January 2009 March 2022 were retrospectively analyzed. Demographic data, clinicopathological characteristics, hematology blood biochemistry baseline results,...

Background NR2F6 is an orphan nuclear receptor with dual tumorigenic activity in the immune system and tumor cells, playing essential role differentiation immunity. This study aimed to investigate expression level of various tumors its effect on neuroblastoma (NB). Methods We evaluated genesis development 34 different through multiple databases. In addition, we investigated effects levels NB risk factors prognosis using pathology sections clinical data from primary retroperitoneal children....

Wilms tumor (WT) is the most common in children. We aim to construct a nomogram predict cancer-specific survival (CSS) of WT children and externally validate China.We downloaded clinicopathological data with from 2004 2018 SEER database. At same time, we used collected previously for all between 2013 at Children's Hospital Chongqing Medical University (Chongqing, China). analyzed difference patients database our hospital. Cox regression analysis was screen significant risk factors. Based on...

Prostate cancer (PC) is the most common non-cutaneous malignancy in men worldwide. Accurate predicting survival of elderly PC patients can help reduce mortality patients. We aimed to construct nomograms predict cancer-specific (CSS) and overall (OS) patients.Information on aged 65 years older was downloaded from Surveillance, Epidemiology, End Results (SEER) database. Univariate multivariate Cox regression models were used determine independent risk factors for Nomograms developed CSS OS...

Background Renal cell carcinoma (RCC) is the most common renal malignant tumor in elderly patients. The prognosis of with distant metastasis poor. We aim to construct a nomogram predict risk patients RCC help doctors and early intervention improve survival rate. Methods clinicopathological information was downloaded from SEER identify all over 65 years old 2010 2018. Univariate multivariate logistic regression analyzed training cohort's independent factors for metastasis. A established based...

Introduction: Neuroblastoma (NB) is one of the most common extracranial solid malignant tumors in children. The 5-year survival rate high-risk or refractory NB less than 50%. Therefore, developing new effective therapeutics for remains an urgent challenge. Materials and Methods: Based on dataset TARGET-NBL TCGA database, prognosis-related genes were analyzed using univariate cox regression (p < 0.01). protein network interaction prognostic was STRING to obtain 150 hub with HR > 1 1....

Osteosarcoma is the most prevalent primary malignant bone tumor. The treatment for osteosarcoma a combination of chemotherapy and surgery. However, there has been no recent progress in role improving long-term survival patients. This study aims to analyze factors that affect patients with explore challenges benefits chemotherapy.Patient data were downloaded from Surveillance, Epidemiology, End Results database. Univariable multivariable logistic regressions used affecting receiving...

Abstract Background Neuroblastoma (NB) is the most common extracranial malignant solid tumor in children. Due to drug resistance radiotherapy and chemotherapy, mainly due existence of cancer stem cells (CSCs), some children still have a poor prognosis. Therefore, researchers focused their attention on CSCs. Our research group successfully constructed cell-like named Piwil2-iCSCs by reprogramming human preputial fibroblasts (FBs) with PIWIL2 gene early stage, were confirmed induce formation...

Bacterial outer membrane vesicles (OMVs) are spherical microbubbles that contain biological content and produced by gram-negative bacteria. The use of OMVs as adjuvants for cancer immunotherapy or drug carriers targeted therapies has attracted the interest many scholars. However, it is unclear whether can exert direct antitumor effects inhibit pediatric tumors. Here, we explore potential Escherichia coli-derived to directly suppress neuroblastoma. Our results demonstrate in vitro vivo, no...

Background Clear cell renal carcinoma (ccRCC) is expected in the elderly and poor prognosis. We aim to explore prognostic factors of ccRCC construct a nomogram predict cancer-specific survival (CSS) patients with ccRCC. Methods Clinicopathological information for all from 2004 2018 was downloaded Surveillance, Epidemiology, End Results (SEER) program. All were randomly assigned training cohort (70%) or validation (30%). Univariate multivariate Cox regression models used identify independent...

Partial bladder outlet obstruction(pBOO) is the most common cause of lower urinary tract symptoms (LUTS) and significantly affects quality life. Long-term pBOO can changes in structure function, referred to as remodeling. The pathogenesis pBOO-induced remodeling has yet be fully understood, so effective treatment options are lacking. Our study aimed explore how brings new strategies for treating pBOO.A rat model was established by partial ligation neck, morphological fibrosis tissues were...

A direct competitive enzyme-linked immunosorbent assay (dcELISA) based on a monoclonal antibody was developed for detecting new β-adrenergic agonist phenylethanolamine (PEAA). PEAA derivative, PEAA–NH2, synthesized by hydrogenation using RANEY® nickel as catalyst, before it coupled to carrier proteins generate PEAA–BSA, PEAA–OVA and PEAA–HRP via diazotization sodium periodate methods, repectively. Three Balb/C mice were immunized with PEAA–BSA the immunogen, three clones stably producing...

Objective To investigate the role of chemokines in Wilms tumours, especially their chemotaxis to immune cells and DNA methylation regulating expression level chemokines. Methods RNAseqV2 gene clinical data were downloaded from TARGET database. GEO cBioPortal The difference analysis Kaplan-Meier(KM) performed by edgeR package. Then predictive model based on was constructed lasso regression multivariate COX regression. ROC curve, DCA Calibration Nomogram used evaluate prognostic model....

With the development of osteosarcoma treatment, limb salvage surgery is gradually replacing amputation as primary surgical option. Most pediatric osteosarcomas limbs undergo limb-salvage surgery. We aimed to use propensity score matching (PSM) analysis test difference in cancer-specific mortality (CSM) between and patients with Osteosarcoma limbs. PSM a statistical method used deal data from an Observational Study. The designed reduce influence biases confounding variables make more...

Objective: To summarize our experiences with drainage methods after laparoscopic pyeloplasty a 14-year study. Methods: We reviewed the data of 838 children operated on for hydronephrosis due to congenital ureteropelvic junction obstruction (UPJO) between July 2007 and 2020. Patients' demographics, perioperative details, postoperative stents [including double-J stent, percutaneous trans-anastomotic (PU) trans-uretero-cystic external urethral stent (TEUS)], complications, hospital stay,...

Background Renal cell carcinoma (RCC) is the most common renal malignancy in adults, and chromophobe (chRCC) third subtype of RCC. We aimed to construct a competitive risk model predict cancer-specific survival (CSS) elderly patients with chRCC. Methods The clinicopathological information was downloaded from SEER database, were randomly divided into training validation cohorts. Patients' factors for death (CSM) analyzed using proportional subdistribution hazard (SH). constructed CSS chRCC...

Circular RNA (circRNA), which is a newly discovered non-coding RNA, has been documented to play important roles in miRNA sponges, and the dysregulation of involved cancer development. However, circRNA expression profiles their role initiation progression Wilms tumor (WT) remain largely unclear at present. Here, we used paired WT samples high-throughput sequencing identify differentially expressed circRNAs (DE-circRs) mRNAs (DE-mRs). A total 314 DE-circRs 1612 DE-mRs were identified. The...