A5064906223- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Algal biology and biofuel production
- Marine and coastal ecosystems
- Photosynthetic Processes and Mechanisms
- Insect Resistance and Genetics
- Biofuel production and bioconversion
- MicroRNA in disease regulation
- Insect and Arachnid Ecology and Behavior
- Scientific Computing and Data Management
- Gene expression and cancer classification
- Clostridium difficile and Clostridium perfringens research
- Agricultural Innovations and Practices
- Neurobiology and Insect Physiology Research
- Advanced biosensing and bioanalysis techniques
- Aquatic Ecosystems and Phytoplankton Dynamics
- Probiotics and Fermented Foods
- Microbial Community Ecology and Physiology
- Mollusks and Parasites Studies
- Insect symbiosis and bacterial influences
- Environmental DNA in Biodiversity Studies
- Thermochemical Biomass Conversion Processes
- RNA Interference and Gene Delivery
- Forest Insect Ecology and Management
- Invertebrate Taxonomy and Ecology
National Renewable Energy Laboratory
2018-2021
University of Idaho
2015-2016
Abstract Microalgae are promising biocatalysts for applications in sustainable fuel, food, and chemical production. Here, we describe culture collection screening, down-selection, development of a high-productivity, halophilic, thermotolerant microalga, Picochlorum renovo . This microalga displays rapid growth rate high diel biomass productivity (34 g m −2 day −1 ), with composition well-suited downstream processing. P. exhibits broad salinity tolerance (growth at 107.5 L salinity)...
Our goal was to explore the accuracy and utility of identifying removing PCR duplicates from HTS data using Super Deduper. Deduper is a pre-alignment, sequence read based technique developed at University Idaho, which examines uses only small portion each read's in order identify remove and/or optical duplicates. Through comparisons with well-known pre- post-alignment techniques, Deduper's parameters were optimized its performance assessed. The results conclude that viable pre-alignment...
High-throughput analysis of biomass is necessary to ensure consistent and uniform feedstocks for agricultural bioenergy applications needed inform genomics systems biology models. Pyrolysis followed by mass spectrometry such as molecular beam (py-MBMS) analyses are becoming increasingly popular the rapid cell wall composition typically require use different data tools depending on need application. Here, authors report py-MBMS several types lignocellulosic gain an understanding spectral...
Artificial microRNA (amiRNA) sequences embedded in natural (miRNA) backbones have proven to be useful tools for RNA interference (RNAi). amiRNAs reduced off-target and toxic effects compared other RNAi-based methods such as short-hairpin RNAs (shRNA). are often less effective knockdown, however, their shRNA counterparts. We screened a large empirically-designed amiRNA set the synthetic inhibitory BIC/miR-155 (SIBR) scaffold show common structural sequence-specific features associated with...
Abstract The larvae of click beetles (Coleoptera: Elateridae), known as “wireworms,” are agricultural pests that pose a substantial economic threat worldwide. We produced one the first wireworm genome assemblies ( Limonius californicus ), and investigated population structure phylogenetic relationships three species L. californicus, infuscatus, canus ) across northwest US southwest Canada using genome-wide markers (RADseq) skimming. found two infuscatus comprised multiple genetically...
Analysis time and storage space are critical bottlenecks when analyzing multi-sample, comparative high throughput sequence experiments. The experimental High Throughput Sequencing (expHTS) pipeline mitigates these by streaming external application data, speeding analysis, reducing eliminating intermediate, un-analyzed files. This creates faster run times, less on the machine, improves uniformity of sample analysis.
We report herein the draft mitochondrial genome sequence of Naesiotus nux, a Galápagos endemic land snail species genus Naesiotus. The circular is 15 kb and encodes 13 protein-coding genes, 2 rRNA 21 tRNA genes.
We report here the full mitochondrial genome sequence of Limonius californicus, a species click beetle that is an agricultural pest in its larval form. The circular 16.5 kb and contains 13 protein-coding genes, 2 rRNA 22 tRNA genes.
Clostridium thermocellum is a thermophilic bacterium recognized for its natural ability to effectively deconstruct cellulosic biomass. While there large body of studies on the genetic engineering this and physiology to-date, limited knowledge in transcriptional regulation organism bacteria general. The study herein first report large-scale application DNA-affinity purification sequencing (DAP-seq) transcription factors (TFs) from bacterium. We applied DAP-seq > 90 TFs C. detected...
Ferredoxin5 (FDX5), a minor ferredoxin protein in the alga Chlamydomonas (Chlamydomonas reinhardtii), helps maintain thylakoid membrane integrity dark. Sulfur (S) deprivation has been used to achieve prolonged hydrogen production green algae. Here, we propose that FDX5 is involved algal responses S-deprivation as well Specifically, tested role of both initial aerobic and subsequent anaerobic phases S-deprivation. Under S-deprived conditions, absence causes distinct delay achieving anoxia by...
DATA REPORT article Front. Bioeng. Biotechnol., 06 March 2018Sec. Bioenergy and Biofuels Volume 6 - 2018 | https://doi.org/10.3389/fbioe.2018.00019