A5007067690- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Ubiquitin and proteasome pathways
- NF-κB Signaling Pathways
- Immune Cell Function and Interaction
- Pain Mechanisms and Treatments
- Hepatitis B Virus Studies
- Neuropeptides and Animal Physiology
- Cervical Cancer and HPV Research
- Hepatitis C virus research
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- RNA modifications and cancer
- Immune Response and Inflammation
- Cancer-related Molecular Pathways
- Cytomegalovirus and herpesvirus research
- RNA regulation and disease
- Plasma Applications and Diagnostics
- Vitamin C and Antioxidants Research
- Neuroscience and Neuropharmacology Research
- Toxoplasma gondii Research Studies
- Cancer-related molecular mechanisms research
- Peptidase Inhibition and Analysis
- Immunodeficiency and Autoimmune Disorders
- Monoclonal and Polyclonal Antibodies Research
Soochow University
2016-2025
Guangzhou Panyu Polytechnic
2025
Guangzhou City Polytechnic
2023-2025
First Affiliated Hospital of Soochow University
2025
University of Electronic Science and Technology of China
2024-2025
Institute of Infection and Immunity
2022
Sun Yat-sen University
2019
Zero to Three
2019
Army Logistics University
2013
Xi'an Jiaotong University
2006-2012
During RNA virus infection, the adaptor protein MAVS recruits TRAF3 and TRAF6 to form a signalosome, which is critical induce production of type I interferons (IFNs) proinflammatory cytokines. While activation MAVS/TRAF3/TRAF6 signalosome well studied, negative regulation remains largely unknown. Here we report that viruses specifically promote deubiquitinase OTUD1 expression by NF-κB-dependent mechanisms at early stage viral infection. Furthermore, upregulates levels intracellular Smurf1...
Abstract Linear ubiquitination is a critical regulator of inflammatory signaling pathways. However, linearly ubiquitinated substrates and the biological significance linear incompletely understood. Here, we show that STAT1 has at Lys511 Lys652 residues in intact cells, which inhibits binding to type-I interferon receptor IFNAR2, thereby restricting activation resulting homeostasis. removed rapidly by OTULIN upon stimulation, facilitates interferon-STAT1 signaling. Furthermore, viruses induce...
Cycloheximide (CHX) is a small molecule derived from Streptomyces griseus that acts as fungicide. As ribosome inhibitor, CHX can restrict the translation elongation of eukaryotic protein synthesis. Once synthesis inhibited by CHX, level intracellular proteins decreases degradation through proteasome or lysosome system. Thus, chase assay widely recognized and used to observe determine half-life given in eukaryotes. Here, we present complete experimental procedure assay. Graphical overview.
STAT1 is a critical transcription factor for regulating host antiviral defenses. activation largely dependent on phosphorylation at tyrosine 701 site of (pY701-STAT1). Understanding how pY701-STAT1 regulated by intracellular signaling remains major challenge. Here we find that the form ubiquitinated-STAT1 induced interferons (IFNs). While total relatively stable during early stages IFNs signaling, can be rapidly downregulated ubiquitin-proteasome system. Moreover, ubiquitinated located...
ABSTRACT DEAD-box helicase (DDX) family members play differential roles in regulating innate antiviral immune response. However, the physiological played by DDX4 immunity remain unclear. In this study, we unveiled that acts as a positive regulatory molecule of Type-I interferon (IFN-I)-mediated activity. Our findings demonstrate IFN-I upregulates protein levels, and subsequently, overexpression enhances IFN-I-mediated signaling pathway. This creates feedback loop amplifies was found to bind...
Abstract Coxsackievirus B3 (CVB3)-induced acute heart failure (AHF) is a common cause of cardiogenic death in young- and middle-aged people. However, the key molecular events linking CVB3 to AHF remain largely unknown, resulting lack targeted therapy strategies thus far. Here, we unexpectedly found that Viperin deficiency does not promote infection but protects mice from CVB3-induced AHF. Importantly, cardiac-specific expression can induce cardiac dysfunction. Mechanistically, CVB3-encoded...
Abstract Ataxin-3 (ATXN3) belongs to the Josephin family of deubiquitinases. So far, ATXN3 is majorly linked neurodegenerative disease, Machado–Joseph disease. The role in antiviral function has not been explored, and vivo deubiquitinating activity remains largely unknown. In this study, we report that an important positive regulator type I IFN (IFN-I)–mediated murine primary lung cells human epithelial fibroblast cell lines. We clarify does promote IFN-I production, but enhances...
Summary Interferon regulatory factor 3 (IRF3) is a critical transcription for inducing production of type I interferons (IFN‐I) and regulating host antiviral response. Although IRF3 activation during viral infection has been extensively studied, the inhibitory regulation remains largely unexplored. Here, we revealed that Midline‐1 (MID1) ubiquitin E3 ligase plays essential roles in IFN‐I. We found MID1 physically interacts with downregulates protein levels. Next, demonstrated can induce...
Healthy behavioral patterns could modulate organ functions to enhance the body's immunity. However, how exercise regulates antiviral innate immunity remains elusive. Here, we found that promotes type I interferon (IFN-I) production in liver and enhances IFN-I immune activity of body. Despite possibility many exercise-induced factors affect production, identified Gpld1 as a crucial molecule, major promote after exercise. Exercise largely loses efficiency induce
With the development of society, energy application and building thermal comfort in rural residences are receiving more attention. The this survey mainly cover areas 21 prefectures Guangdong province, which 24.7% Pearl River Delta, 18.9% western Guangdong, 13.1% eastern 43.2% northern Guangdong. Rural household consumption is used for lighting equipment, appliances, cooking where equipment appliances consume electrical energy, consumes different types due to diversity types. First, there a...
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) generally hijacks the cellular machinery of host cells for survival. However, how SARS-CoV-2 employs host’s deubiquitinase to facilitate virus replication remains largely unknown. In this study, we identified USP22 as a crucial regulator expression nucleocapsid protein (SARS-CoV-2 NP), which is essential replication. We demonstrated that NP proteins undergo ubiquitination-dependent degradation in cells, while interacts...
Interferons (IFNs) have broad-spectrum antiviral activity to resist virus epidemic. However, IFN efficacy needs be greatly improved. Here, we reveal that LATS1 is a vital signal transmitter governing full type-I (IFN-I) signaling activity. constitutively binds with the IFN-I receptor IFNAR2 and rapidly tyro-phosphorylated by Tyk2 upon engagement. Tyro-phosphorylation of promotes activation YAP degradation, thereby promoting IFN-mediated antiproliferation Moreover, activated translocates into...