A5065843661- Gene expression and cancer classification
- Complement system in diseases
- Blood Coagulation and Thrombosis Mechanisms
- Genomics and Phylogenetic Studies
- Cancer Genomics and Diagnostics
- Genomic variations and chromosomal abnormalities
- Diatoms and Algae Research
- Microbial Community Ecology and Physiology
- Bioinformatics and Genomic Networks
- Genomics and Rare Diseases
- Genetics, Bioinformatics, and Biomedical Research
- Scientific Computing and Data Management
- Algorithms and Data Compression
- Algal biology and biofuel production
- Genome Rearrangement Algorithms
- Plant Molecular Biology Research
- Bayesian Methods and Mixture Models
- Platelet Disorders and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Polysaccharides and Plant Cell Walls
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cardiac Fibrosis and Remodeling
- Innovative Microfluidic and Catalytic Techniques Innovation
- Kawasaki Disease and Coronary Complications
Université de Rouen Normandie
2004-2023
Normandie Université
2016-2022
Inserm
1989-2022
Laboratoire d'Informatique, du Traitement de l'Information et des Systèmes
2009-2022
Centre Henri Becquerel
2020
Institute for Research and Innovation in Biomedicine
2014-2015
Institute for Biomedicine
2014-2015
Centre National de la Recherche Scientifique
2001
Centre Hospitalier Universitaire de Rouen
1995
Universität Innsbruck
1992
Discriminating which nucleotide variants cause disease or contribute to phenotypic traits remains a major challenge in human genetics. In theory, any intragenic variant can potentially affect RNA splicing by altering regulatory elements (SREs). However, these alterations are often ignored mainly because pioneer SRE predictors have proved inefficient. Here, we report the first large-scale comparative evaluation of four user-friendly SRE-dedicated algorithms (QUEPASA, HEXplorer, SPANR, and...
Cardiac lymphatics have emerged as potential targets in cardiovascular diseases (CVDs). However, we recently reported that despite extensive lymphatic expansion during experimental cardiac pressure-overload, drainage remained insufficient. To unravel the cellular and molecular mechanisms underlying dysfunction CVDs, applied single-cell (sc) analyses a murine heart failure model. Transaortic constriction (TAC), C57BL/6J BALB/c mice, was used to model chronic pressure-overload-induced...
Abstract Motivation: As more and whole genomes are available, there is a need for new methods to compare large sequences transfer biological knowledge from annotated related ones. BLAST not suitable multimegabase DNA sequences. MegaBLAST designed closely Some tools detect repeats in have already been developed such as MUMmer or REPuter. They also time space restrictions. Moreover, terms of applications, REPuter only computes works better with genomes. Results: We present heuristic method,...
Abstract We have studied the secretion of proteins alternative pathway complement C3, factor B and H by human umbilical vein endothelial cells (HUVEC). Results showed that are quantitatively secreted in abundance whereas C3 could only be detected when maintained culture during long periods time. Interferon‐γ stimulated H, and, to a lesser extent, secretions. Interleukin (IL) 1 had differential effect on spontaneous In presence IL 1, there was significant occurring within short period...
Abstract Motivation Next-generation sequencing has become the go-to standard method for detection of single-nucleotide variants in tumor cells. The use such technologies requires a PCR amplification step and step, steps which artifacts are introduced at very low frequencies. These often confused with true low-frequency that can be found cells cell-free DNA. recent unique molecular identifiers (UMI) targeted protocols offered trustworthy approach to filter out artefactual accurately call...
Abstract We have studied the secretion of complement regulatory protein factor I by human umbilical vein endothelial cells (HUVEC). Northern and Western blot analysis biosynthetic labeling experiments indicate that HUVEC secrete at very low levels in basal conditions this is significantly enhanced interferon‐γ. Analysis proteolytic inactivation C3b supernatants show secreted a functional form can promote specific to iC3b. Together with previous studies establishing H HUVEC, work demonstrates...
Phaeodactylum tricornutum is the most studied diatom encountered principally in coastal unstable environments. It has been hypothesized that great adaptability of P. probably due to its pleomorphism. Indeed, an atypical since it can display three morphotypes: fusiform, triradiate and oval. Currently, little information available regarding physiological significance this morphogenesis. In study, we adapted Pt3 strain obtain algal culture particularly enriched one dominant morphotype: or These...
SUMMARY Constitutive secretion of complement C3 and factor B by the endothelial cell (EC) is lowered therapeutic concentrations glucocorticoids such as hydrocortisone or dexamethasone, whereas regulatory protein H production increased these hormones. In contrast, pro-inflammatory cytokine IL-1α has a stimulatory effect on endothelium an inhibitory secretion. this study, we examined combined expression cell. When dexamethasone were added to IL-1α, significant potentialization IL-1α-induced...
Abstract The effect of the synthetic glucocorticoid dexamethasone (DXM) on secretion by human monocytes alternative complement proteins C3, factor B and H was investigated. Results indicated that DXM modulates this in a direction which would be consistent with its anti‐inflammatory properties. DXM, at therapeutic concentrations, had suppressive C3 stimulatory monocytes. This differential modulation similar mature macrophages. Together previous studies showing endothelial cells, our results...
Abstract Background Whole exome sequencing (WES) has become the strategy of choice to identify a coding allelic variant for rare human monogenic disorder. This approach is revolution in medical genetics history, impacting both fundamental research, and diagnostic methods leading personalized medicine. A plethora efficient algorithms been developed ensure discovery. They generally lead ~20,000 variations that have be narrow down find potential pathogenic variant(s) affected gene(s). For this...
Abstract Background Recently, copy number variations (CNV) impacting genes involved in oncogenic pathways have attracted an increasing attention to manage disease susceptibility. CNV is one of the most important somatic aberrations genome tumor cells. Oncogene activation and suppressor gene inactivation are often attributed gain/amplification or deletion, respectively, many cancer types stages. Recent advances next generation sequencing protocols allow for addition unique molecular...
SUMMARY We have studied complement activation both in plasma samples and In lesional skin from palients with leukocytoclaslic cutaneous vasculitis (LCV). Enzyme immunoassay (EIA) quantification of the markers. C3d,g fhe terminal complex (TCC) plasma, showed lhal their levels were significantly increased 66% 55% patienis, respectively (n = 29) compared healthy controls, whereas standard measurements C3, factor B, Clq, C4and C2 generally within normal range. Elevations TCC signifieantly...
Detection of repeated sequences within complete genomes is a powerful tool to help understanding genome dynamics and species evolutionary history. To distinguish significant repeats from those that can be obtained just by chance, statistical methods have developed. In this paper we show the distribution number long in generated stationary Markov chains approximated Poisson with explicit parameter. Thanks Chen-Stein method provide bound for approximation error; converges 0 as soon length n...
Summary Objective: To summarize excellent current research and propose a selection of best papers published in 2016 the field Bioinformatics Translational Informatics with applications health domain clinical care. Methods: We provide synopsis articles selected for IMIA Yearbook 2017, from which we attempt to derive synthetic overview future activities field. As 2016, first step was performed by querying MEDLINE list MeSH descriptors completed terms adapted section coverage. Each editor...
Summary Objectives : To summarize excellent current research and propose a selection of best papers published in 2015 the field Bioinformatics Translational Informatics with application health domain clinical care. Method We provide synopsis articles selected for IMIA Yearbook 2016, from which we attempt to derive synthetic overview future activities field. As last year, first step was performed by querying MEDLINE list MeSH descriptors completed terms adapted section. Each section editor...
Detection of repeated sequences within complete genomes is a powerful tool to help understanding genome dynamics and species evolutionary history. To distinguish significant repeats from those that can be obtained just by chance, statistical methods have developed. In this paper we show the distribution number long in generated stationary Markov chains approximated Poisson with explicit parameter. Thanks Chen-Stein method provide bound for approximation error; converges 0 as soon length n...
Summary Objective: To summarize excellent current research and propose a selection of best papers published in 2016 the field Bioinformatics Translational Informatics with applications health domain clinical care. Methods: We provide synopsis articles selected for IMIA Yearbook 2017, from which we attempt to derive synthetic overview future activities field. As 2016, first step was performed by querying MEDLINE list MeSH descriptors completed terms adapted section coverage. Each editor...