A5004423646- Pharmacogenetics and Drug Metabolism
- Dermatology and Skin Diseases
- Gut microbiota and health
- Computational Drug Discovery Methods
- Metabolomics and Mass Spectrometry Studies
- Redox biology and oxidative stress
- Drug-Induced Hepatotoxicity and Protection
- Infant Health and Development
- Drug Transport and Resistance Mechanisms
- COVID-19 Clinical Research Studies
- Glutathione Transferases and Polymorphisms
- Advanced Proteomics Techniques and Applications
- Asthma and respiratory diseases
- Cancer Treatment and Pharmacology
- Food Allergy and Anaphylaxis Research
- Toxic Organic Pollutants Impact
- Diet and metabolism studies
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Histone Deacetylase Inhibitors Research
- Melanoma and MAPK Pathways
- Antibiotics Pharmacokinetics and Efficacy
- Treatment of Major Depression
- Sulfur Compounds in Biology
- COVID-19 and healthcare impacts
Agency for Science, Technology and Research
2020-2025
Rutgers, The State University of New Jersey
2025
National Skin Centre
2022
National Cancer Centre Singapore
2022
National University of Singapore
2011-2020
Evidence is accumulating that the establishment of gut microbiome in early life influences development atopic eczema. In this longitudinal study, we used integrated multi-omics analyses to infer functional mechanisms by which modulates eczema risk. We measured functionality and metabolome 63 infants between ages 3 weeks 12 months with well-defined cases controls a sub-cohort from Growing Up Singapore Toward healthy Outcomes (GUSTO) mother-offspring cohort. At weeks, allergen-sensitized were...
ABSTRACT Ceramide‐based and aqueous‐based moisturizers have distinct absorption retention characteristics in skin. Conventional methods like Transepidermal Water Loss (TEWL) Corneometry are limited by environmental factors, no current vivo method measures ceramide content This research explores the use of a handheld Confocal Raman Spectroscopy (CRS) system to non‐invasively analyze kinetics these moisturizers. Measurements were taken on 40 subjects, including healthy individuals ( n = 20)...
Short chain fatty acids (SCFAs) are the main intestinal intermediate and end products of metabolism dietary fibers/polyphenols by gut microbiota. The aim this study was to evaluate biological implication stool SCFA profiles determined in first year life on clinical presentation allergic outcomes childhood.From Growing Up Singapore Toward healthy Outcomes (GUSTO) cohort, a sub-cohort 75 participants recruited. Scheduled questionnaire data collected for cumulative prevalence...
Perfluorooctanoic acid (PFOA) is an environmental toxicant exhibiting a years-long biological half-life (t1/2) in humans and linked with adverse health effects. However, limited understanding of its toxicokinetics (TK) has obstructed the necessary risk assessment. Here, we constructed first middle-out physiologically based toxicokinetic (PBTK) model to mechanistically explain persistence PFOA humans. In vitro transporter kinetics were thoroughly characterized scaled up vivo clearances using...
Although covalent protein binding is established as the pivotal event underpinning acetaminophen (APAP) toxicity, its mechanistic details remain unclear. In this study, we demonstrated that APAP induces widespread glutathionylation in a time-, dose- and bioactivation-dependent manner HepaRG cells. Proteo-metabonomic mapping provided evidence APAP-induced resulted functional deficits energy metabolism, elevations oxidative stress cytosolic calcium, well mitochondrial dysfunction correlate...
Short chain fatty acids (SCFAs) are postulated to modulate the immune development of neonates via epigenetic regulations such as histone deacetylase (HDAC) inhibition. In context atopic diseases, inhibition HDAC maintains T-cell homeostasis and induces naïve differentiation into adaptive Treg, which regulates production anti-inflammatory cytokines suppression Th2 responses. We investigated structure-inhibition relationships SCFAs with class I HDAC3 IIa HDAC7 using in silico docking...
Leber’s hereditary optic neuropathy (LHON, MIM#535000) is the most common form of inherited neuropathies and mitochondrial DNA-related diseases. The pathogenicity mutations in genes encoding components Complex I well established, but underlying pathomechanisms disease are still unclear. Hypothesizing that oxidative stress related to deficiency may increase protein S-glutathionylation, we investigated proteome-wide S-glutathionylation profiles LHON (n = 11) control 7) fibroblasts, using...
The isotope-coded affinity tag (ICAT) technique has been applied to measure pairwise changes in protein expression through differential stable isotopic labeling of proteins or peptides followed by identification and quantification using a mass spectrometer. Changes are observed when the identical peptide from each two biological conditions is identified difference detected measurements comparing labeled with heavy isotope one normal distribution. This approach allows simultaneous comparison...
Lopinavir/ritonavir, originally developed for treating HIV, is currently undergoing clinical studies the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Although recent reports suggest that lopinavir exhibits in vitro efficacy against SARS-CoV-2, it a highly protein-bound drug and remains unknown if reaches adequate vivo unbound (free) concentrations lung tissue. We built physiologically-based pharmacokinetic model of lopinavir/ritonavir white Chinese populations. Our aim was...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected half a billion people, including vulnerable populations such as cancer patients. While increasing evidence supports the persistence of SARS-CoV-2 months after negative nasopharyngeal swab test, effects on long-term immune memory and treatment are unclear. In this report, we examined post-COVID-19 tissue-localized responses in hepatocellular carcinoma (HCC) patient colorectal (CRC) patient. Using spatial...