A5008032941- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- Vibrio bacteria research studies
- Pesticide Exposure and Toxicity
- Cholinesterase and Neurodegenerative Diseases
- Micro and Nano Robotics
- bioluminescence and chemiluminescence research
- Diffusion and Search Dynamics
- Molecular Sensors and Ion Detection
- Bacteriophages and microbial interactions
- Chemical Reaction Mechanisms
- Antimicrobial Peptides and Activities
- Bacillus and Francisella bacterial research
- Chemical Reactions and Mechanisms
- Computational Drug Discovery Methods
- Biochemical and Structural Characterization
- Protist diversity and phylogeny
Weizmann Institute of Science
1989-2020
Institut Pasteur
2018-2020
Abstract Historically, multicellular bacterial communities, known as biofilms, have been thought to be held together solely by a self-produced extracellular matrix. Our study identified novel mechanism maintaining Bacillus subtilis and Mycobacterium smegmatis biofilms—active production of calcite minerals. We studied, for the first time, effects mutants defective in biomineralization formation on biofilm development, resilience morphology. demonstrated that an intrinsic rise carbon dioxide...
In response to chemical communication, bacterial cells often organize themselves into complex multicellular communities that carry out specialized tasks. These are frequently referred as biofilms, which involve collective behavior of different cell types. Like eukaryotes, the biofilm surrounded by self-produced polymers constitute extracellular matrix (ECM), binds them each other and surface. it has been evident for decades cell-ECM interactions control multiple cellular processes during...
Bacillus subtilis biofilms have a fundamental role in shaping the soil ecosystem. During this process, they unavoidably interact with neighbour bacterial species. We studied interspecies interactions between of soil-residing bacteria B. and related found that proximity triggered recruitment motile cells, which engulfed competing simplex colony. Upon interaction, secreted surfactin cannibalism toxins, at concentrations were inert to itself, eliminated colony, as well colonies toyonensis....
The matrix protein TasA maintains a subpopulation of motile cells within Bacillus subtilis biofilms.
Toxin-antitoxin modules are gene pairs encoding a toxin and its antitoxin, found on the chromosomes of many bacteria, including pathogens. Here, we characterize specific contribution TxpA YqcG toxins in elimination defective cells from developing Bacillus subtilis biofilms. On nutrient limitation, accumulated biofilm breaking symmetry. Deletion resulted accumulation morphologically abnormal cells, interfered with proper development multicellular community. Dual physiological responses...
Bacteria in nature often reside differentiated communities termed biofilms, which are an active interphase between uni-cellular and multicellular life states for bacteria. Here we demonstrate that the development of B. subtilis biofilms is dependent on use glutamine or glutamate as a nitrogen source. We show differential metabolic requirement within biofilm; while necessary dividing cells at edges, inner cell mass utilizes lactic acid. Our results indicate biofilm preserve short-term memory...
Bacteria in nature are usually found complex multicellular structures, called biofilms. One common form of a biofilm is pellicle-a floating mat bacteria formed the water-air interphase. So far, our knowledge on basic mechanisms underlying formation biofilms at air-liquid interfaces not complete. In particular, co-occurrence motile cells and extracellular matrix producers has been studied. addition, potential involvement chemical communication pellicle remained largely undefined. Our results...
Homologous aged and nonaged fluorescent organophosphorus conjugates of alpha-chymotrypsin (Cht) were used in a comparative spectroscopic study the conformation their active sites, employing pyrene group as probe. Steady-state fluorescence measurements showed that quantum yield probe which is stoichiometrically attached to site ca. 20% lower conjugate, pyrenebutyl-O-P(O)(O-)-Cht (PBP-Cht), than pyrenebutyl-O-P-(O)(OC2H5)-Cht (PBEP-Cht). Furthermore, decay data indicate quenching dynamic not...
Summary Bacteria in nature are usually found complex multicellular communities, termed biofilms. Biofilms generally seen as sessile structures, resulting from downregulation of motility. However, during interspecies competition and predation, biofilm cells were shown to migrate towards competitor colonies. Here, we show that a specific extracellular matrix (ECM) protein, TasA, is essential for collective migration toward potential competitors serves developmental cue increases the formation...