A5062316483- Gut microbiota and health
- Inflammatory Bowel Disease
- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Nutrition, Genetics, and Disease
- Metabolism and Genetic Disorders
- Mycobacterium research and diagnosis
- Lipid metabolism and disorders
- Liver Disease Diagnosis and Treatment
- Neonatal Health and Biochemistry
- Medical and Biological Ozone Research
- Gestational Diabetes Research and Management
- Prenatal Substance Exposure Effects
- Neutrino Physics Research
- Immune responses and vaccinations
- Nuclear Physics and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Photodynamic Therapy Research Studies
- Urinary Tract Infections Management
- Acupuncture Treatment Research Studies
- Diagnosis and treatment of tuberculosis
- Ferroptosis and cancer prognosis
- Biochemical Analysis and Sensing Techniques
- Infections and bacterial resistance
- Cancer-related molecular mechanisms research
Sheba Medical Center
1986-2025
Tel Aviv University
1986-2024
Edmond and Lily Safra Children's Hospital
2021-2024
Weizmann Institute of Science
1998-2020
A major challenge in understanding gene regulation is the unequivocal identification of enhancer elements and uncovering their connections to genes. We present GeneHancer, a novel database human enhancers inferred target genes, framework GeneCards. First, we integrated total 434 000 reported from four different genome-wide databases: Encyclopedia DNA Elements (ENCODE), Ensembl regulatory build, functional annotation mammalian genome (FANTOM) project VISTA Enhancer Browser. Employing an...
Background & AimsEnvironmental enteric dysfunction (EED) limits the Sustainable Development Goals of improved childhood growth and survival. We applied mucosal genomics to advance our understanding EED.MethodsThe Study Environmental Enteropathy Malnutrition (SEEM) followed 416 children from birth 24 months in a rural district Pakistan. Biomarkers were measured at 9 tested for association with months. The duodenal methylome transcriptome determined 52 undernourished SEEM participants 42 North...
Abstract The coronavirus disease 2019 (COVID-19) has rapidly spread around the world, impacting lives of many individuals. Growing evidence suggests that nasopharyngeal and respiratory tract microbiome are influenced by various health conditions, including presence severity different viral disease. To evaluate potential interactions between Severe Acute Respiratory Syndrome Corona 2 (SARS-CoV-2) microbiome. Microbial composition swab samples submitted to clinical microbiology lab for...
Abstract The human gut microbiome develops during the first years of life, followed by a relatively stable adult microbiome. Day care attendance is drastic change that exposes children to large group peers in diverse environment for prolonged periods, at this critical time microbial development, and therefore has potential affect composition. We characterize effect day on development throughout single school year 61 from 4 different facilities, additional 24 age-matched home ( n = 268...
Abstract Crohn disease (CD) burden has increased with globalization/urbanization, and the rapid rise is attributed to environmental changes rather than genetic drift. The Study Of Urban Rural CD Evolution (SOURCE, n = 380) considered diet-omics domains simultaneously detect complex interactions identify potential beneficial pathogenic factors linked rural-urban transition CD. We characterize exposures, diet, ileal transcriptomics, metabolomics, microbiome in newly diagnosed patients controls...
The matrix protein TasA maintains a subpopulation of motile cells within Bacillus subtilis biofilms.
Spinal cord injury (SCI) is a devastating event that significantly changes daily function and quality of life linked to bowel bladder dysfunction frequent antibiotic treatment. We aimed study the composition gut microbiome in individuals with SCI during initial sub-acute rehabilitation process chronic phase injury. This included 100 fecal samples from 63 participants (Median age 40 years, 94% males): 13 cases 50 longitudinal samples, 18 SCI, 32 gender-matched controls. show, using...
Abstract Background Characterizing gut factors that improve or persist during Crohn’s disease (CD) trajectory from active to remission healthy-normal states is crucial for defining treatment targets and CD cure. Methods Using multi-omics (ileal transcriptomics, microbiome, metabolomics), we aimed compare active, mostly naïve newly diagnosed patients in remission, using controls as a reference Results 193 subjects were included (median age 32 years, 60% male); 78 38 (80%) patients, 77 non-IBD...
Abstract Background IgA is the predominant antibody in gut, coating a larger proportion of intestinal bacteria. IgG found lower concentrations but tends to increase with gut inflammation. We aimed characterize IgA- and IgG-coated microbial compositions healthy controls evaluate variations shared bacteria across individuals. Methods established protocol for isolating IgG-bound fecal bacteria, using magnetic streptavidin beads coated biotin anti-human or (Fig. 1A). The initial stool, as well...
Perturbagen analysis of Crohn's disease (CD) ileal gene expression data identified small molecules including eicosatetraynoic acid (ETYA), which may exert an antifibrotic effect. We developed a patient-specific human intestinal organoid (HIO) model system to test molecule regulation mitochondrial and wound-healing functions implicated in stricturing behavior.
Ulcerative colitis (UC), Crohn's disease (CD), and celiac are prevalent intestinal inflammatory disorders with nonsatisfactory therapeutic interventions. Analyzing patient data-driven cohorts can highlight pathways new targets for Long noncoding RNAs (lncRNAs) attractive candidates, since they readily targetable by RNA therapeutics, show relative cell-specific expression, play key cellular functions. Uniformly analyzing gut mucosal transcriptomics from 696 subjects, we have highlighted...
We aimed to determine microbial signature linked with lung cancer (LC) diagnosis and define taxa durable clinical benefit (DCB) of advanced LC patients. Stool samples for 16S amplicon sequencing data were collected from 75 patients (50 which treated checkpoint inhibitors) 31 matched healthy volunteers. compared controls DCB those without. had lower α-diversity higher between-subject diversity. Random Forests model differentiate cases ROC-AUC was 0.74. Clostridiales, Lachnospiraceae,...
Abstract Background Metabolites, produced endogenously or derived from the gut microbiome, are key mediators and effectors of many cellular functions, but there only scant data on their temporal changes along CD course. We analyzed dynamics serum fecal metabolome in a prospective cohort quiescent patients over 2 years until occurrence clinical flare, aiming to identify whether precede predict relapse. Methods Untargeted metabolomics was performed using LC/MS. Multivariate analyses MaAsLin2...
Gel lubrication is routinely used during gynecological examination to prevent or reduce pain, yet its impact on microbial composition sampling remains unclear. This study aimed investigate whether lubricating gel affects the of vaginal samples. We included 31 pregnant women presenting their third trimester clinics emergency room and collected 143 unique samples for 16S amplicon analysis. Vaginal were obtained using sterile swabs under various conditions: without gel-immediately frozen (n =...
High fructose consumption is one of the hallmarks Western diets and has been found to induce MeS symptoms in parallel gut microbial dysbiosis. However, causality between those two still elusive. Here, we studied whether a significant modification composition by antibiotics can influence fructose-induced metabolic changes. Male Sprague-Dawley (SD) rats were divided into four groups including controls, controls + antibiotics, high diet (HFrD, 60% fructose), HFrD (n = 7–8 each group) for period...
Retinitis pigmentosa (RP) is a genetic blinding disease with over 80 causative genes. Disease progression varies between patients similar backgrounds. We assessed the association environment, gut microbiota, and retinal degeneration in RP rat model Royal College of Surgeons (RCS). The rats were born raised for two generations under specific pathogen-free (SPF, n = 69) or non-SPF conditions (n 48). At age four weeks, SPF had significantly shorter dark-adapted a-wave dark light-adapted b-wave...
Abstract Background The prevalence of metabolic and immune-mediated diseases has increased in the last century, with many these showing alterations gut microbial composition. We previously reported (PMID:35197084) that a large group bacteria shows constant differential abundance (increase decrease) across multiple can therefore provide general indication host's health. aimed to utilize “health index” based on compare how different exposures are associated this index as potential contributors...
Abstract Background The increase in prevalence of Crohn Disease (CD) worldwide together with suboptimal results current therapies mandate identification new targets. We aimed to identify dietary components and metabolites linked host microbial factors support targeting beneficial exposures. Methods Analyses the SOURCE Israeli sub-cohort included clinical, biomarkers, computerized food frequency questionnaire (FFQ), fecal metabolomics, mucosal terminal ileum (TI) transcriptomics, microbiome....