A5035931522- Micro and Nano Robotics
- Spectroscopy Techniques in Biomedical and Chemical Research
- nanoparticles nucleation surface interactions
- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Plasmonic and Surface Plasmon Research
- Lipid Membrane Structure and Behavior
- Reproductive Biology and Fertility
- Physiological and biochemical adaptations
- Photoreceptor and optogenetics research
- Pickering emulsions and particle stabilization
- Advanced Antenna and Metasurface Technologies
- Material Dynamics and Properties
- Electromagnetic Scattering and Analysis
- Bacteriophages and microbial interactions
- Marine Invertebrate Physiology and Ecology
- Biocrusts and Microbial Ecology
- Molecular Communication and Nanonetworks
- Spaceflight effects on biology
- Solidification and crystal growth phenomena
- Gold and Silver Nanoparticles Synthesis and Applications
- Modular Robots and Swarm Intelligence
- Escherichia coli research studies
- thermodynamics and calorimetric analyses
Hebrew University of Jerusalem
2009-2024
Ben-Gurion University of the Negev
2023-2024
Institut Curie
2012-2023
Sorbonne Université
2018-2023
Université Paris Sciences et Lettres
2018-2023
Centre National de la Recherche Scientifique
2018-2023
Physique des Cellules et Cancers
2021-2023
Czech Academy of Sciences, Institute of Physics
2012
Abstract Activity and autonomous motion are fundamental in living engineering systems. This has stimulated the new field of ‘active matter’ recent years, which focuses on physical aspects propulsion mechanisms, motility-induced emergent collective behavior a larger number identical agents. The scale agents ranges from nanomotors microswimmers, to cells, fish, birds, people. Inspired by biological various designs synthetic nano- micromachines have been proposed. Such machines provide basis...
We investigate the large length and long time scales collective flows structural rearrangements within in vitro human bronchial epithelial cell (HBEC) cultures. Activity-driven result ensembles of vortices randomly positioned space. By analyzing a population vortices, we show that their area follows an exponential law with constant mean value rotational frequency is size independent, both being characteristic features chaotic dynamics active nematic suspensions. Indeed, find HBECs...
Bacterial adhesins are modular cell-surface proteins that mediate adherence to other cells, surfaces, and ligands. The Antarctic bacterium Marinomonas primoryensis uses a 1.5-MDa adhesin comprising over 130 domains position it on ice at the top of water column for better access oxygen nutrients. We have reconstructed this 0.6-μm-long using "dissect build" structural biology approach established complementary roles its five distinct regions. Domains in region I (RI) tether type secretion...
Abstract Hydrostatic skeletons such as the Hydra's consist of two stacked layers muscle cells perpendicularly oriented. In vivo, these bilayers first assemble, and then fibers both develop organize with this crisscross orientation. present work, we identify an alternative mechanism bilayering myoblasts in vitro, which results from prior local organization active initial monolayer. The myoblast sheet can be described a contractile nematic which, expected, most +1/2 topological defects...
Successfully cryopreserving cells adhered to a substrate would facilitate the growth of vital confluent cell culture after thawing while dramatically shortening post-thaw culturing time. Herein we propose controlled slow cooling method combining initial directional freezing followed by gradual down -80°C for robust preservation monolayers adherent substrate. Using computer cryostages examined effect rates and dimethylsulfoxide (DMSO) concentration on survival established an optimal...
Cancerous human fibrosarcoma cells undergo collective migration at the edge of their colony by combining mesoscale self-organization and intrinsic handedness, showing how physical interactions alone can lead to biological functionality.
We discuss the Surface-Plasmon-Resonance (SPR) technique based on Fourier -Transform - InfraRed (FTIR) spectrometry. explore potential of infrared surface plasmon resonance for biological studies in aqueous solutions and compare it to conventional operating visible range. demonstrate that sensitivity SPR range is not lower fact even higher. show several examples applying FTIR-SPR studies: (i) monitoring D-glucose concentration solution, (ii) measuring uptake by erythrocytes suspension....
Topological defects, which are singular points in a director field, play major role shaping the multi-layered structure of swarm.
Cell morphology is often used as a valuable indicator of the physical condition and general status living cells. We demonstrate noninvasive method for morphological characterization adherent measure infrared reflectivity spectrum at oblique angle from cells cultured on thin Au film, utilize unique properties confined waves (i.e., surface plasmon guided modes) traveling inside cell layer. The propagation these strongly depends connectivity. By tracking resonant wavelength attenuation modes we...
Ice-binding proteins (IBPs) are expressed in various organisms for several functions, such as protecting them from freezing and freeze injuries. Via adsorption on ice surfaces, IBPs depress growth recrystallization affect nucleation shaping. have shown promise mitigating under moderate supercooling conditions, but their functionality cryogenic conditions has been less explored. In this study, we investigate the impact of two types antifreeze (AFPs): type III AFP fish a hyperactive an insect,...
We demonstrate that a live epithelial cell monolayer can act as planar waveguide. Our infrared reflectivity measurements show highly differentiated simple cells, which maintain tight intercellular connectivity, support efficient waveguiding of the light in spectral region 1.4-2.5 µm and 3.5-4 µm. The wavelength magnitude waveguide mode resonances disclose quantitative dynamic information on height cell-cell connectivity. To this we two experiments. In first one trace real-time kinetics...
Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation V. Yashunsky, A. Zilberstein, T. Marciano, Lirtsman, M. Golosovsky, D. Davidov, B. Aroeti; Infrared Surface Plasmon Spectroscopy of Living Cells. AIP Conference Proceedings 30 September 2010; 1281 (1): 1617–1621. https://doi.org/10.1063/1.3498133 Download citation file: Ris (Zotero) Reference Manager EasyBib...
Enteropathogenic Escherichia coli (EPEC) is an important, generally non-invasive, bacterial pathogen that causes diarrhea in humans. The microbe infects mainly the enterocytes of small intestine. Here we have applied our newly developed infrared surface plasmon resonance (IR-SPR) spectroscopy approach to study how EPEC infection affects epithelial host cells. IR-SPR experiments showed results a robust reduction refractive index infected Assisted by confocal and total internal reflection...
The cell morphology is a valuable indicator of the physical condition and general status cell. Here we demonstrate methodology for noninvasive biosensing adherent living cells. Our method based on infrared reflection spectroscopy cells cultured thin Au film. To characterize utilized unique properties surface plasmon (λ=1-3 μm) guided wave that travel inside monolayer. We our enables monitoring submicron variations in real-time labelfree manner. In addition to morphological characterization,...
High-power illumination of thin ice crystals drives the formation dynamic holes and water microchannel labyrinths.
Abstract Simple hydrostatic skeletons such as the Hydra’s consist of two stacked layers cells perpendicularly oriented. Although this crisscross architecture can be recapitulated in vitro, little is known on formation multilayers starting from a monolayer. In present article, we show that bilayering myoblasts results organization and activity originally monolayer which described contractile active nematic. As expected, most +1/2 topological defects are associated with nematic order...
Topological defects, which are singular points in a director field, play major role shaping active systems. Here, we experimentally study topological defects and the flow patterns around them, that formed during highly rapid dynamics of swarming bacteria. The results compared to predictions two-dimensional nematics. We show that, even though some assumptions underlying theory do not hold, swarm is agreement with nematic theory. In particular, look into multi-layered structure swarm, an...