A5079907424- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Erythrocyte Function and Pathophysiology
- Bacterial Genetics and Biotechnology
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bacteriophages and microbial interactions
- Pancreatic function and diabetes
- Vibrio bacteria research studies
- Caveolin-1 and cellular processes
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Hepatitis C virus research
- Antibiotic Resistance in Bacteria
- Glycosylation and Glycoproteins Research
- Plasmonic and Surface Plasmon Research
- Advanced Biosensing Techniques and Applications
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Listeria monocytogenes in Food Safety
- Plant Virus Research Studies
- Endoplasmic Reticulum Stress and Disease
Hebrew University of Jerusalem
2014-2024
Institute of Life Sciences
2000-2012
Stanford University
2007
Jerusalem College of Technology
2001
University of California, San Francisco
1992-1996
Tel Aviv University
1986-1992
Previous studies of fibroblasts have demonstrated that recycling endocytic receptors occurs through a default mechanism membrane‐volume sorting. Epithelial cells require an additional level polar membrane sorting, but there are conflicting models some suggesting it in early endosomes, others specialized apical endosome (ARE). The relationship between sorting to the lysosomal, and transcytotic pathways polarized was addressed by characterizing itineraries LDL, transferrin (Tf) IgA,...
Quantitative confocal microscopic analyses of living, polarized MDCK cells demonstrate different pH profiles for apical and basolateral endocytic pathways, despite a rapid extensive intersection between the two. Three‐dimensional characterizations ligand trafficking that pathways share early, acidic compartments distributed throughout medial regions cell. Polar sorting both occurs in these common endosomes as IgA is sorted from transferrin to alkaline transcytotic vesicles. While directly...
The 17-juxtamembrane cytoplasmic residues of the polymeric immunoglobulin receptor contain an autonomous basolateral targeting signal that does not mediate rapid endocytosis (Casanova, J. E., G. Apodaca, and K. E. Mostov. Cell. 66:65-75). Alanine-scanning mutagenesis identifies three in this region, His656, Arg657, Val660, are most essential for sorting two residues, Arg655 Tyr668, play a lesser role process. Progressive truncations suggested Ser664 Ile665 might also sorting. However,...
Phosphatidylinositol 4,5-bisphosphate [PI(4,5)P(2)] and phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P(3)] are phosphoinositides (PIs) present in small amounts the inner leaflet of plasma membrane (PM) lipid bilayer host target cells. They thought to modulate activity proteins involved enteropathogenic Escherichia coli (EPEC) infection. However, role PI(4,5)P(2) PI(3,4,5)P(3) EPEC pathogenesis remains obscure. Here we show that induces a transient accumulation at bacterial infection...
GLUT2 is a facilitative glucose transporter, expressed in polarized epithelial cells of the liver, intestine, kidney and pancreas, where it plays critical role homeostasis. Together with SGLT1/2, mediates absorption metabolic tissues, can be translocated apically upon high exposure. To track subcellular localization dynamics GLUT2, we created an mCherry-hGLUT2 fusion protein multicellular cysts, major site reabsorption. Live imaging enabled us to avoid artefactual fixed confirm apical model....
ABSTRACT Hepatitis C virus (HCV) is an important cause of liver disease worldwide. Current therapies are inadequate for most patients. Using a two-hybrid screen, we isolated novel cellular binding partner interacting with the N terminus HCV nonstructural protein NS5A. This contains TBC Rab-GAP (GTPase-activating protein) homology domain found in all known Rab-activating proteins. As first described interaction between such and viral protein, this finding suggests new mechanism whereby...
We discuss the Surface-Plasmon-Resonance (SPR) technique based on Fourier -Transform - InfraRed (FTIR) spectrometry. explore potential of infrared surface plasmon resonance for biological studies in aqueous solutions and compare it to conventional operating visible range. demonstrate that sensitivity SPR range is not lower fact even higher. show several examples applying FTIR-SPR studies: (i) monitoring D-glucose concentration solution, (ii) measuring uptake by erythrocytes suspension....
The ability of diarrheagenic bacterial pathogens, such as enteropathogenic Escherichia coli (EPEC), to modulate the activity mitogen-activated protein kinases (MAPKs) and cell survival has been suggested benefit colonization infection. However, our understanding mechanisms by which EPEC these functions is incomplete. In this study, we show that type III secreted effector Map stimulates sheddase disintegrin metalloproteinase domain-containing 10 (ADAM10) ERK p38 MAPK signaling cascades....
The compartments involved in polarized exocytosis of membrane proteins are not well defined. In this study we hypothesized that newly synthesized polymeric immunoglobulin receptors targeted from the <i>trans</i>-Golgi network to endosomes prior their appearance on basolateral cell surface Madin-Darby canine kidney cells. To examine hypothesis, have used an assay designed measure meeting with a selective population apical or loaded horseradish peroxidase. We found course exocytosis, wild-type...
Enterohaemorrhagic Escherichia coli and enteropathogenic E. are enteropathogens characterized by their ability to induce the host cell form actin-rich structures, termed pedestals. A type III secretion system, through which pathogens deliver effector proteins into infected cells, is essential for virulence pedestal formation. encodes two similar effectors, EspM1 EspM2, activate RhoA signalling pathway formation of stress fibres upon infection cells. We confirm these observations in addition...
Enteropathogenic Escherichia coli (EPEC) is an important human pathogen that causes acute infantile diarrhea. The type IV bundle-forming pili (BFP) of typical EPEC strains are dynamic fibrillar organelles can extend out and retract into the bacterium. bfpF gene encodes for BfpF, a protein promotes retraction. BFP involved in bacterial autoaggregation mediating initial adherence bacterium with its host cell. Importantly, retraction implicated virulence experimental infection. How contributes...
ABSTRACT We hypothesized that ADP-ribosylation factor 1 (Arf1) plays an important role in the biogenesis and maintenance of infectious hepatitis C virus (HCV). Huh7.5 cells, which HCV replicates produces viral particles, were exposed to brefeldin A or golgicide A, pharmacological inhibitors Arf1 activation. Treatment with these agents caused a reduction RNA levels, accumulation particles within levels extracellular medium. Fluorescence analyses showed nonstructural (NS) proteins NS5A NS3,...
ABSTRACT A subpopulation of hepatitis C virus (HCV) core protein in cells harboring full-length HCV replicons is biochemically associated with detergent-resistant membranes (DRMs) a manner similar to that markers classical lipid rafts. Core does not, however, colocalize immunofluorescence studies plasma membrane raft markers, such as caveolin-1 and the B subunit cholera toxin, suggesting bound cytoplasmic microdomains distinct from caveolin-based Furthermore, while both structural...
We provide morphological, biochemical, and functional evidence suggesting that the AP-1 clathrin adaptor complex of trans-Golgi network interacts with polymeric immunoglobulin receptor in transfected Madin-Darby canine kidney cells. Our results indicate immunofluorescently labeled γ-adaptin subunit partially co-localize polarized semi-polarized γ-Adaptin is co-immunoisolated membranes expressing wild-type receptor. The entire could be chemically cross-linked to filter-grown...
ABSTRACT Basolateral sorting of the polymeric immunoglobulin receptor (pIgR) expressed in Madin-Darby canine kidney (MDCK) cells is mediated by a 17-residue signal that resides cytoplasmic domain. We have recently analyzed sequence requirements alanine scanning mutagenesis. found basolateral primarily three amino acids: H656, R657 and V660. Individual mutations each these residues to Ala caused substantial decrease corresponding increase targeting apical surface. Structural analysis peptides...
Enteropathogenic E. coli (EPEC) is an extracellular diarrheagenic human pathogen which infects the apical plasma membrane of small intestinal enterocytes. EPEC utilizes a type III secretion system to translocate bacterial effector proteins into its epithelial hosts. This activity, subverts numerous signaling and trafficking pathways in infected cells, thought contribute virulence. The molecular cellular mechanisms underlying these events are not well understood. We investigated mode by...