A5068573837- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Syphilis Diagnosis and Treatment
- Infectious Diseases and Mycology
- Bacterial Identification and Susceptibility Testing
- SARS-CoV-2 and COVID-19 Research
- Streptococcal Infections and Treatments
- Sinusitis and nasal conditions
- Infective Endocarditis Diagnosis and Management
- Escherichia coli research studies
- Plant Pathogens and Fungal Diseases
- Bacterial Genetics and Biotechnology
- SARS-CoV-2 detection and testing
- Microbial infections and disease research
- COVID-19 and healthcare impacts
- Bartonella species infections research
- Rabies epidemiology and control
- Reproductive tract infections research
- Pneumocystis jirovecii pneumonia detection and treatment
- Biosensors and Analytical Detection
- Biofield Effects and Biophysics
- Eosinophilic Esophagitis
- Nail Diseases and Treatments
- Mycobacterium research and diagnosis
- Chronic Kidney Disease and Diabetes
University of Washington
2012-2025
Seattle University
2018-2025
University of Washington Medical Center
2017-2023
Catholic University of the Maule
2023
Pontificia Universidad Católica de Chile
2023
University of Alabama at Birmingham
2023
Centers for Disease Control and Prevention
2023
Thomas Jefferson University
2022
Ducks Unlimited
2021
University of Maryland, Baltimore
2011-2017
Multiple laboratory-developed tests (LDTs) and commercially available assays have emerged to meet diagnostic needs related the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. To date, there is limited comparison data for these different testing platforms. We compared analytical performance of a LDT developed in our clinical laboratory based on CDC primer sets four available, FDA emergency use authorized SARS-CoV-2 (Cepheid, DiaSorin, Hologic Panther, Roche Cobas) total...
Plasmodium ookinetes traverse midgut epithelial cells before they encounter the complement system in mosquito hemolymph. We identified a heme peroxidase (HPX2) and NADPH oxidase 5 (NOX5) as critical mediators of nitration antiplasmodial immunity that enhance nitric oxide toxicity Anopheles gambiae. show two immune mechanisms target ookinetes-epithelial thioester-containing protein 1 (TEP1)-mediated lysis-work sequentially, we propose works an opsonization-like promotes activation cascade.
The Group A Streptococcus remains a significant human pathogen causing wide array of disease ranging from self-limiting to life-threatening invasive infections. Epithelium (skin or throat) colonization with progression the subepithelial tissues is common step in all GAS Here, we used transposon-sequencing (Tn-seq) define 5448 genetic requirements for vivo fitness tissue. near-saturation transposon library M1T1 strain was injected subcutaneously into mice, producing suppurative inflammation...
Broad-range fungal PCR is a powerful tool for identifying pathogens directly from patient specimens; however, reported estimates of clinical utility vary and costs discourage universal testing. We investigated the diagnostic broad-range by examining 9 years results sinonasal specimens, hypothesizing that this anatomic location would identify immunocompromised patients at high risk invasive disease. retrospectively identified 644 PCRs 1,446 cultures sinus sites. To determine relative...
Abstract Background Syphilis cases continue to increase rapidly in the US. Molecular detection of pathogen, Treponema pallidum (TP) may speed turnaround time (TAT) and resolve ambiguous results from current diagnostics methods, which require stepwise serologic testing. Here we describe validation clinical performance a loop-mediated isothermal amplification (LAMP) assay for TP. Methods Candidate LAMP primers targeting 23S rRNA locus TP were evaluated best performing set was used per Clinical...
We identified 34 patients with Coxiella burnetii infection using PCR; 31 (86%) cases were diagnosed from cardiac specimens. Nearly half (15/31, 48%) of those not reported to any channel national disease surveillance, indicating substantial underreporting for diseases molecular methods at noncommercial laboratories.
Enteropathogenic Escherichia coli (EPEC) is an important human pathogen that causes acute infantile diarrhea. The type IV bundle-forming pili (BFP) of typical EPEC strains are dynamic fibrillar organelles can extend out and retract into the bacterium. bfpF gene encodes for BfpF, a protein promotes retraction. BFP involved in bacterial autoaggregation mediating initial adherence bacterium with its host cell. Importantly, retraction implicated virulence experimental infection. How contributes...
Significance This work used mass spectrometry imaging to visualize a key bacterial virulence factor, lipid A within tissues infected by Francisella novicida , model organism for tularensis ssp. the causative agent of tularemia. Lipid components host were mapped using same technique, leading identification lethal role metabolism in infection. Combined with profiling inflammatory mediators, this defined dramatic polarity cyclooxygenase-2 axis infection and overproduction proinflammatory...
Sexual transmission of enteric pathogens among men who have sex with (MSM) is well documented, although whether providers are cognizant this risk when MSM patients present gastrointestinal symptoms has not been studied. Over 34 months at a major tertiary metropolitan medical system, study retrospectively analyzed 436 BioFire FilmArray Gastrointestinal results from 361 documented as MSM. An extensive chart review was performed, including specific sexual behaviors, socioeconomic factors, and...
The first coronavirus disease 2019 (COVID-19) case in the United States was reported Washington State. pandemic caused drastic disruptions to medical institutions, including education. Department of Laboratory Medicine at University responded by rapidly implementing substantial changes student clerkships.In real time, we converted one ongoing case- and didactic-based course, LabM 685, remote learning.Fifteen 17 scheduled sessions proceeded as planned, two for presentations. Two didactics...
Type IV pili (T4P) are filamentous surface appendages required for tissue adherence, motility, aggregation, and transformation in a wide array of bacteria archaea. The bundle-forming pilus (BFP) enteropathogenic Escherichia coli (EPEC) is prototypical T4P confirmed virulence factor. fibers assembled by complex biogenesis machine that extrudes through an outer membrane (OM) pore formed the secretin protein. Secretins constitute superfamily proteins assemble into multimers support transport...
The broad-range detection and identification of bacterial DNA from clinical specimens are a foundational approach in the practice molecular microbiology. However, there circumstances under which conventional testing may yield false-negative or otherwise uninterpretable results, including presence multiple templates degraded nucleic acids. Here, we describe an alternative, next-generation sequencing for broad range using 16S rRNA gene hybrid capture (“16S Capture”).
Molecular assays for infectious diseases have emerged as important clinical decision-making tools. Unbiased, metagenomic next-generation sequencing is a novel approach holding promise to detect pathogens missed by conventional modalities and deconvolute admixed nucleic acid sequences from polymicrobial infections in order identify constituent pathogens. Recent studies raised concerns about the impact of metagenomics whether their expense justified. Here, we report case polyclonal...