A5054823577- Cannabis and Cannabinoid Research
- Glycosylation and Glycoproteins Research
- Neuroscience and Neuropharmacology Research
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Diet, Metabolism, and Disease
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience of respiration and sleep
- Analytical Chemistry and Chromatography
- Sleep and Wakefulness Research
- Atherosclerosis and Cardiovascular Diseases
- Biochemical effects in animals
- Metabolism and Genetic Disorders
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Alcohol Consumption and Health Effects
- Dietary Effects on Health
- Lipid Membrane Structure and Behavior
- Cancer, Lipids, and Metabolism
- Birth, Development, and Health
- Microfluidic and Capillary Electrophoresis Applications
- Erythrocyte Function and Pathophysiology
- Pancreatic function and diabetes
- Tryptophan and brain disorders
University Medical Center of the Johannes Gutenberg University Mainz
2016-2025
Johannes Gutenberg University Mainz
2016-2025
University of Applied Sciences Mainz
2021
Ludwig-Maximilians-Universität München
2018
Tampere University Hospital
2018
Tampere University
2018
Maastricht University Medical Centre
2018
University of Turku
2018
UNSW Sydney
2015
Institute of Biophysics
2011
Significance A runner’s high is a subjective sense of well-being some humans experience after prolonged exercise. For decades, it was hypothesized that exercise-induced endorphin release solely responsible for high, but recent evidence has suggested endocannabinoids also may play role. Here, we demonstrate wheel running increases and reduces both anxiety sensation pain in mice. Ablation cannabinoid receptor 1 receptors on GABAergic neurons inhibits running-induced anxiolysis, pharmacological...
Dysregulated adipocyte physiology leads to imbalanced energy storage, obesity, and associated diseases, imposing a costly burden on current health care. Cannabinoid receptor type-1 (CB1) plays crucial role in controlling metabolism through central peripheral mechanisms. In this work, adipocyte-specific inducible deletion of the CB1 gene (Ati-CB1–KO) was sufficient protect adult mice from diet-induced obesity metabolic alterations reverse phenotype already obese mice. Compared with controls,...
Abstract Lipidomics encompassing automated lipid extraction, a four-dimensional (4D) feature selection strategy for confident annotation as well reproducible and cross-validated quantification can expedite clinical profiling. Here, we determine 4D descriptors (mass to charge, retention time, collision cross section, fragmentation spectra) of 200 standards 493 lipids from reference plasma via trapped ion mobility mass spectrometry enable the implementation stringent criteria annotation. We...
Social affiliation is essential for many species and gains significant importance during adolescence. Disturbances in social affiliation, particular rejection experiences adolescence, affect an individual's well-being are involved the emergence of psychiatric disorders. The underlying mechanisms still unknown, partly because a lack valid animal models. By using novel model peer-rejection, which compromises adolescent rats their ability to appropriately engage playful activities, here we...
Objective- Palmitoylethanolamide is an endogenous fatty acid mediator that synthetized from membrane phospholipids by N-acyl phosphatidylethanolamine phospholipase D. Its biological actions are primarily mediated PPAR-α (peroxisome proliferator-activated receptors α) and the orphan receptor GPR55. exerts potent anti-inflammatory but its physiological role promise as a therapeutic agent in chronic arterial inflammation, such atherosclerosis remain unexplored. Approach Results- First,...
Significance This study discloses specific central and peripheral mechanisms involving cannabinoid type-1 (CB1) receptors in impairing the effect of acute stress on consolidation nonemotional memory rodents. Both hippocampal CB1 dopamine β-hydroxylase–expressing cells (i.e., adrenergic/noradrenergic cells) are identified as necessary sufficient for stress-induced impairment. Our provides foundation development novel potential approaches to tackle cognitive alterations stress-related disorders.
Endocannabinoid signaling via anandamide (AEA) is implicated in a variety of neuronal functions and considered promising therapeutic target for numerous emotion-related disorders. The major AEA degrading enzyme fatty acid amide hydrolase (FAAH). Genetic deletion pharmacological inhibition FAAH reduce anxiety improve emotional responses memory rodents humans. Complementarily, the mechanisms impact decreased remain to be delineated detail. In present study, using Cre/loxP system combined with...
Gilles de la Tourette syndrome (TS) is a complex neurodevelopmental disorder characterized by the presence of motor and vocal tics as well psychiatric comorbidities such attention-deficit/hyperactivity (ADHD), obsessive-compulsive (OCD), depression, anxiety. The underlying cause disease still unknown, but several lines evidence suggest paramount role dopaminergic system. Based on clinical observation that cannabis-based medicine including cannabis delta-9-tetrahydrocannabinol (THC,...
Resource10 October 2022Open Access Transparent process Lipid and protein content profiling of isolated native autophagic vesicles Daniel Schmitt The Autophagy Lab, Institute Pathobiochemistry, University Medical Center the Johannes Gutenberg University, Mainz, Germany Contribution: Conceptualization, Data curation, Formal analysis, Validation, Investigation, Writing - review & editing Search for more papers by this author Süleyman Bozkurt orcid.org/0000-0003-4965-3195 Biochemistry II,...
The introduction of chip-based electrospray (ESI) ion sources into biological mass spectrometry (MS) addressed the fundamental issue how to analyze minute amounts complex systems. automation sample delivery MS combined with ESI allows for high quality bioanalysis in a high-throughput fashion. These advantages have already been demonstrated proteomics, direct screening drugs and drug discovery. As part our continuing effort implement automated field carbohydrate analysis, we hereby report...
Gangliosides (GGs), involved in malignant alteration and tumor progression/invasiveness, are considered as biomarkers or therapeutic targets. Here, we describe the first systematic GG composition characterization human gliosarcoma versus normal brain tissue using our recently developed mass spectrometry (MS) methods, based on nano‐electrospray (nano‐ESI), Fourier-transform ion cyclotron resonance (FT-ICR), chip nano‐ESI quadrupole time-of-flight (QTOF), complemented by thin-layer...
Significance Hypoxic pulmonary vasoconstriction (HPV) is an important physiological reflex, which only found in the lung and adapts perfusion to ventilation. HPV potentially involved hypoxia-induced hypertension (PH) occurring respiratory disorders. In this study we show that endocannabinoid anandamide (AEA) via its fatty acid amide hydrolase (FAAH)-dependent metabolites PH. We have identified arterial smooth muscle cells as source of AEA synthesis. Our results illustrate onset PH prevented...
Endocannabinoids participate in the control of neurogenesis, neural cell death and gliosis. The pharmacological effect fatty acid amide hydrolase (FAAH) inhibitor URB597, which limits endocannabinoid degradation, was investigated present study. Cell proliferation (phospho-H3+ or BrdU+ cells) main adult neurogenic zones as well apoptosis (cleaved caspase-3+), astroglia (GFAP+), microglia (Iba1+ were analyzed hippocampus, hypothalamus striatum rats intraperitoneally treated with URB597 (0.3...
Postsynaptically generated 2-arachidonoylglycerol activates the presynaptic cannabinoid type-1 receptor, which is involved in synaptic plasticity at both glutamatergic and GABAergic synapses. However, differential function of signaling vs synapses context animal behavior has not been investigated yet.Here, we analyzed role selectively hippocampal neurons. Monoacylglycerol lipase, primary degrading enzyme 2-arachidonoylglycerol, expressed sites excitatory inhibitory By adeno-associated...
Abstract Anxiety- and trauma-related disorders are severe illnesses with high prevalence. Current treatment options leave room for improvement the endocannabinoid system (ECS) has become a key target in psychopharmacological research. Rodent models suggest an anxiolytic effect of endocannabinoids demonstrated that ECS is involved modulation fear learning aversive memory consolidation. So far, one prominent was inhibition fatty acid amino hydrolase (FAAH), degrading enzyme anandamide (AEA)....
Heart failure is a multifaceted clinical syndrome, in which the heart fails to supply adequate blood meet body's oxygen and nutrients needs. Evidence indicates multi-level molecular shifts subjects, necessitating unbiased stratification of patients with failure. This study utilized AI-based multimodal integration method analyse 359 lipids 538 proteins measured participants MyoVasc cohort. Patient similarity networks were constructed, spectral clustering, an unsupervised machine learning...
The integration of multi-omics data offers transformative potential for elucidating complex molecular mechanisms underlying biological processes and diseases. In this study, we developed a lipid–metabolite–protein network that combines protein–protein interaction enzymatic genetic interactions proteins with metabolites lipids to provide unified framework integration. Using hyperbolic embedding, the visualizes connections across omics layers, accessible through user-friendly Shiny R (version...